Click here to switch to the map view.

The map label for this gene is truA

Identifier: 15669871

GI number: 15669871

Start: 1658647

End: 1659426

Strand: Direct

Name: truA

Synonym: MJ1675

Alternate gene names: 15669871

Gene position: 1658647-1659426 (Clockwise)

Preceding gene: 15669860

Following gene: 15669872

Centisome position: 99.62

GC content: 33.72

Gene sequence:

>780_bases
ATGTATATTCTAAAAATCGCCTACGATGGAAGATACAGCTTTCAACAACAGCCACATAAACAAACAGTTTGTGATATTTT
GTTAGATGCCTTAGAAGAAACTGGATTTTTAGCTAAAAAGAAGGTTATATACAGCGGAGGAAGGACAGATAAAGGCGTCT
CTGCCTTAGGCAACTTTGTAGTTGTAGAGCTGAAAAAAGAGCCAATCCTCTCATACATAAATGCAAAGCTAAAGGATAAA
GGCATCTGGGTTTTAGGATATAGAGAGATTGATGAAATTCCTAAGGTGAAGTATAGACATTACAGATATATTCTCCCAAA
CATTGGTTATGATGTAGATGCAATAAAAGAAGGAGCTAAGAAGATGATTGGCACTCATTCCTTCCACAATCTATCAAAGA
GAGATAGAACAAAAGAAAAAAGTCCAATAAGAACAATCTATGATATAAAAATCTCTGAAAATGAGTTCTATTTAACAGTA
GATATCATTGGAGAGAGCTTTTTATGGAACATGGTCAGAAAGATAGTTGGAGCTTTAGATTTAGTTGGAAGAGGAAAAAA
GCCAGTTGAATGGATTGATAAGCTTTTAGATGAAAACCATAGAGAAGGAGTCCCTACATTTCCACCAGAAGGGTTGATAT
TGGTTGAGGCAAAAGTAGATATCGAATATATATACGATAATTACTCAATAAGAAAATTTAAAGAGTATTGGGGGGAGTTC
TTTAAATTGCAAGTTATGAAAATAGGAATCGCTAAAACAGTTTTAGAATTTACAAAATAG

Upstream 100 bases:

>100_bases
TTTGTGGAACCTTAAAAATAATAAAAAAGTCATATAAATAGTTTTTTATTTTGTAAATTTGAAAATATACAAATATGAAT
TAATTTTAAGGTGGTAATTT

Downstream 100 bases:

>100_bases
ATAATTTTATTGTTTAGTTTAAATGTGCCCCGGGGGAGCAAAGGGGACTCACAGCTTGCCGGGCTGTCGCTCATCCCCCG
CGGTTTCCCGGGGCATTTTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MYILKIAYDGRYSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFVVVELKKEPILSYINAKLKDK
GIWVLGYREIDEIPKVKYRHYRYILPNIGYDVDAIKEGAKKMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTV
DIIGESFLWNMVRKIVGALDLVGRGKKPVEWIDKLLDENHREGVPTFPPEGLILVEAKVDIEYIYDNYSIRKFKEYWGEF
FKLQVMKIGIAKTVLEFTK

Sequences:

>Translated_259_residues
MYILKIAYDGRYSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFVVVELKKEPILSYINAKLKDK
GIWVLGYREIDEIPKVKYRHYRYILPNIGYDVDAIKEGAKKMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTV
DIIGESFLWNMVRKIVGALDLVGRGKKPVEWIDKLLDENHREGVPTFPPEGLILVEAKVDIEYIYDNYSIRKFKEYWGEF
FKLQVMKIGIAKTVLEFTK
>Mature_259_residues
MYILKIAYDGRYSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFVVVELKKEPILSYINAKLKDK
GIWVLGYREIDEIPKVKYRHYRYILPNIGYDVDAIKEGAKKMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTV
DIIGESFLWNMVRKIVGALDLVGRGKKPVEWIDKLLDENHREGVPTFPPEGLILVEAKVDIEYIYDNYSIRKFKEYWGEF
FKLQVMKIGIAKTVLEFTK

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Escherichia coli, GI1788657, Length=253, Percent_Identity=27.6679841897233, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321112, Length=173, Percent_Identity=27.1676300578035, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_METJA (Q59069)

Other databases:

- EMBL:   L77117
- PIR:   A64509
- RefSeq:   NP_248685.1
- ProteinModelPortal:   Q59069
- SMR:   Q59069
- GeneID:   1452584
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1675
- NMPDR:   fig|243232.1.peg.1722
- TIGR:   MJ1675
- HOGENOM:   HBG621020
- OMA:   KIAYDGR
- ProtClustDB:   PRK00021
- BioCyc:   MJAN243232:MJ_1675-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30110; Mature: 30110

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: ACT_SITE 50-50

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYILKIAYDGRYSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFV
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCCHHHHCCCEE
VVELKKEPILSYINAKLKDKGIWVLGYREIDEIPKVKYRHYRYILPNIGYDVDAIKEGAK
EEEECCCHHHHHHCCCCCCCCEEEEECHHHHCCCCCCHHHEEEECCCCCCCHHHHHHHHH
KMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTVDIIGESFLWNMVRKIVGALD
HHHCCCHHHCCHHHHCCCCCCCCEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHH
LVGRGKKPVEWIDKLLDENHREGVPTFPPEGLILVEAKVDIEYIYDNYSIRKFKEYWGEF
HHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHH
FKLQVMKIGIAKTVLEFTK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYILKIAYDGRYSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFV
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCCHHHHCCCEE
VVELKKEPILSYINAKLKDKGIWVLGYREIDEIPKVKYRHYRYILPNIGYDVDAIKEGAK
EEEECCCHHHHHHCCCCCCCCEEEEECHHHHCCCCCCHHHEEEECCCCCCCHHHHHHHHH
KMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTVDIIGESFLWNMVRKIVGALD
HHHCCCHHHCCHHHHCCCCCCCCEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHH
LVGRGKKPVEWIDKLLDENHREGVPTFPPEGLILVEAKVDIEYIYDNYSIRKFKEYWGEF
HHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHH
FKLQVMKIGIAKTVLEFTK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087