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The map label for this gene is sepS [H]

Identifier: 15669856

GI number: 15669856

Start: 1641851

End: 1643500

Strand: Direct

Name: sepS [H]

Synonym: MJ1660

Alternate gene names: 15669856

Gene position: 1641851-1643500 (Clockwise)

Preceding gene: 15669855

Following gene: 15669859

Centisome position: 98.61

GC content: 34.0

Gene sequence:

>1650_bases
ATGAAATTAAAACATAAAAGGGATGATAAAATGAGATTTGATATAAAAAAGGTTTTAGAGTTAGCAGAGAAGGATTTTGA
GACGGCATGGAGAGAGACAAGGGCATTAATAAAGGATAAACATATTGACAATAAATATCCAAGATTAAAGCCTGTCTATG
GAAAGCCACATCCAGTGATGGAGACGATAGAGAGATTAAGACAAGCTTATCTAAGAATGGGATTTGAAGAGATGATTAAT
CCAGTTATCGTTGATGAGATGGAGATTTATAAGCAATTTGGACCAGAAGCAATGGCAGTTTTAGATAGATGTTTTTACTT
GGCTGGATTACCAAGGCCAGATGTTGGTTTAGGAAATGAGAAGGTTGAGATTATAAAAAATTTGGGCATAGATATAGATG
AGGAGAAAAAAGAGAGGTTGAGAGAAGTTTTACATTTATACAAAAAAGGAGCTATAGATGGGGATGATTTAGTCTTTGAG
ATTGCCAAAGCTTTAAATGTGAGTAATGAAATGGGATTGAAGGTTTTAGAAACTGCATTTCCTGAATTTAAAGATTTGAA
GCCAGAATCAACAACTCTAACTTTAAGAAGCCACATGACATCTGGGTGGTTTATAACTCTAAGCAGTTTAATAAAGAAGA
GAAAACTGCCTTTAAAGTTATTCTCTATAGATAGATGTTTTAGAAGGGAGCAAAGAGAGGATAGAAGCCATTTAATGAGT
TATCACTCTGCATCTTGTGTAGTTGTTGGTGAAGATGTTAGTGTAGATGATGGAAAGGTAGTTGCTGAAGGATTGTTGGC
TCAATTTGGATTTACAAAATTTAAGTTTAAGCCAGATGAGAAAAAGAGTAAGTATTATACACCAGAAACTCAAACAGAGG
TTTATGCCTATCATCCAAAGTTGGGAGAGTGGATTGAAGTAGCAACCTTTGGAGTTTATTCACCAATTGCATTAGCTAAA
TATAACATAGATGTGCCAGTTATGAACCTTGGCTTAGGAGTTGAGAGGTTGGCAATGATTATTTACGGCTATGAGGATGT
TAGGGCAATGGTTTATCCTCAATTTTATGAATACAGGTTGAGTGATAGAGATATAGCTGGGATGATAAGAGTTGATAAAG
TTCCTATATTGGATGAATTCTACAACTTTGCAAATGAGCTTATTGATATATGCATAGCAAATAAAGATAAGGAAAGCCCA
TGTTCAGTTGAAGTTAAAAGGGAATTCAATTTCAATGGGGAGAGAAGAGTAATTAAAGTAGAAATATTTGAGAATGAACC
AAATAAAAAGCTTTTAGGTCCTTCTGTGTTAAATGAGGTTTATGTCTATGATGGAAATATATATGGCATTCCGCCAACGT
TTGAAGGGGTTAAAGAACAGTATATCCCAATTTTAAAGAAAGCTAAGGAAGAAGGAGTTTCTACAAACATTAGATACATA
GATGGGATTATCTATAAATTAGTAGCTAAGATTGAAGAGGCTTTAGTTTCAAATGTGGATGAATTTAAGTTCAGAGTCCC
AATAGTTAGAAGTTTGAGTGACATAAACCTAAAAATTGATGAATTGGCTTTAAAACAGATAATGGGGGAGAATAAGGTTA
TAGATGTTAGGGGACCAGTTTTCTTAAATGCAAAGGTTGAGATAAAATAG

Upstream 100 bases:

>100_bases
GTTTGGACTGTTAAGAAAAACATCCTATTTGTGAATATTGAGGAAGGCATCTTAAAGCCTCAAAGTAAATTAGATTTATT
AGCAATTAGAGAGATATTAG

Downstream 100 bases:

>100_bases
AAATAATCTTTTAATCTTTTATTTTTTAACACAGTTTTCAACAAAATACTTATAAACCTTATATCCATCTTCAGATAGCT
CTGGATGGAATGATAGAGCC

Product: O-phosphoseryl-tRNA synthetase

Products: NA

Alternate protein names: SepRS; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKLKHKRDDKMRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVMETIERLRQAYLRMGFEEMIN
PVIVDEMEIYKQFGPEAMAVLDRCFYLAGLPRPDVGLGNEKVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFE
IAKALNVSNEMGLKVLETAFPEFKDLKPESTTLTLRSHMTSGWFITLSSLIKKRKLPLKLFSIDRCFRREQREDRSHLMS
YHSASCVVVGEDVSVDDGKVVAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPKLGEWIEVATFGVYSPIALAK
YNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRLSDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESP
CSVEVKREFNFNGERRVIKVEIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQYIPILKKAKEEGVSTNIRYI
DGIIYKLVAKIEEALVSNVDEFKFRVPIVRSLSDINLKIDELALKQIMGENKVIDVRGPVFLNAKVEIK

Sequences:

>Translated_549_residues
MKLKHKRDDKMRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVMETIERLRQAYLRMGFEEMIN
PVIVDEMEIYKQFGPEAMAVLDRCFYLAGLPRPDVGLGNEKVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFE
IAKALNVSNEMGLKVLETAFPEFKDLKPESTTLTLRSHMTSGWFITLSSLIKKRKLPLKLFSIDRCFRREQREDRSHLMS
YHSASCVVVGEDVSVDDGKVVAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPKLGEWIEVATFGVYSPIALAK
YNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRLSDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESP
CSVEVKREFNFNGERRVIKVEIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQYIPILKKAKEEGVSTNIRYI
DGIIYKLVAKIEEALVSNVDEFKFRVPIVRSLSDINLKIDELALKQIMGENKVIDVRGPVFLNAKVEIK
>Mature_549_residues
MKLKHKRDDKMRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVMETIERLRQAYLRMGFEEMIN
PVIVDEMEIYKQFGPEAMAVLDRCFYLAGLPRPDVGLGNEKVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFE
IAKALNVSNEMGLKVLETAFPEFKDLKPESTTLTLRSHMTSGWFITLSSLIKKRKLPLKLFSIDRCFRREQREDRSHLMS
YHSASCVVVGEDVSVDDGKVVAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPKLGEWIEVATFGVYSPIALAK
YNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRLSDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESP
CSVEVKREFNFNGERRVIKVEIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQYIPILKKAKEEGVSTNIRYI
DGIIYKLVAKIEEALVSNVDEFKFRVPIVRSLSDINLKIDELALKQIMGENKVIDVRGPVFLNAKVEIK

Specific function: Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys) [H]

COG id: COG2024

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit (archaeal type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=162, Percent_Identity=35.1851851851852, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32563657, Length=158, Percent_Identity=33.5443037974684, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508957, Length=158, Percent_Identity=33.5443037974684, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321087, Length=172, Percent_Identity=31.9767441860465, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI18858079, Length=144, Percent_Identity=36.8055555555556, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR005246
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.27 [H]

Molecular weight: Translated: 63434; Mature: 63434

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKHKRDDKMRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
ETIERLRQAYLRMGFEEMINPVIVDEMEIYKQFGPEAMAVLDRCFYLAGLPRPDVGLGNE
HHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCH
KVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFEIAKALNVSNEMGLKVLETAF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCHHHHHHHC
PEFKDLKPESTTLTLRSHMTSGWFITLSSLIKKRKLPLKLFSIDRCFRREQREDRSHLMS
CHHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
YHSASCVVVGEDVSVDDGKVVAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPK
HCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEEECCC
LGEWIEVATFGVYSPIALAKYNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRL
CCCHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHCCHHHHHHHHCHHHHHHCC
SDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESPCSVEVKREFNFNGERRVIKV
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEE
EIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQYIPILKKAKEEGVSTNIRYI
EEEECCCCCCCCCHHHHCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
DGIIYKLVAKIEEALVSNVDEFKFRVPIVRSLSDINLKIDELALKQIMGENKVIDVRGPV
HHHHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHCCEEHHHHHHHHHHCCCCEEEECCCE
FLNAKVEIK
EEEEEEEEC
>Mature Secondary Structure
MKLKHKRDDKMRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
ETIERLRQAYLRMGFEEMINPVIVDEMEIYKQFGPEAMAVLDRCFYLAGLPRPDVGLGNE
HHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCH
KVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFEIAKALNVSNEMGLKVLETAF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCHHHHHHHC
PEFKDLKPESTTLTLRSHMTSGWFITLSSLIKKRKLPLKLFSIDRCFRREQREDRSHLMS
CHHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
YHSASCVVVGEDVSVDDGKVVAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPK
HCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEEECCC
LGEWIEVATFGVYSPIALAKYNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRL
CCCHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHCCHHHHHHHHCHHHHHHCC
SDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESPCSVEVKREFNFNGERRVIKV
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEE
EIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQYIPILKKAKEEGVSTNIRYI
EEEECCCCCCCCCHHHHCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
DGIIYKLVAKIEEALVSNVDEFKFRVPIVRSLSDINLKIDELALKQIMGENKVIDVRGPV
HHHHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHCCEEHHHHHHHHHHCCCCEEEECCCE
FLNAKVEIK
EEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]