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The map label for this gene is glyA

Identifier: 15669792

GI number: 15669792

Start: 1568600

End: 1569889

Strand: Direct

Name: glyA

Synonym: MJ1597

Alternate gene names: 15669792

Gene position: 1568600-1569889 (Clockwise)

Preceding gene: 15669789

Following gene: 15669796

Centisome position: 94.21

GC content: 36.51

Gene sequence:

>1290_bases
ATGGAATATTCGGATGTTCCAAAGTTTATTAGGGACGTTTCAATAAAACAGCACGAGTGGATGAGAGAGAGCATAAAATT
AATTGCAAGTGAGAATATAACAAGTTTAGCAGTTAGAGAGGCGTGTGCAACAGATTTTATGCATAGATATGCTGAGGGAT
TACCAGGAAAAAGATTATACCAAGGATGTAAATATATAGATGAAGTTGAAACACTCTGTATAGAGTTGTCTAAAGAATTA
TTTAAAGCTGAACACGCAAACGTTCAGCCAACAAGTGGTGTCGTTGCTAATTTAGCTGTTTTCTTTGCTGAAACAAAGCC
AGGGGATAAATTAATGGCTTTAAGTGTTCCAGATGGTGGGCATATAAGCCATTGGAAAGTAAGTGCTGCTGGAATTAGAG
GATTAAAAGTTATAAACCATCCATTCGACCCAGAAGAGATGAATATTGATGCTGATGCAATGGTTAAAAAAATCTTAGAA
GAGAAGCCAAAGCTAATATTGTTTGGAGGTTCTTTATTCCCATTCCCTCATCCAGTAGCTGATGCCTATGAAGCTGCTCA
AGAAGTTGGTGCTAAAATTGCCTATGATGGAGCTCATGTTTTGGGATTAATAGCTGGAAAGCAGTTCCAAGACCCATTGA
GAGAAGGGGCGGAGTATTTAATGGGTAGCACTCATAAAACATTCTTTGGTCCTCAAGGAGGAGTTATATTAACAACAAAA
GAAAACGCTGACAAGATAGACAGCCATGTATTCCCAGGGGTTGTTAGTAACCATCATTTACATCATAAGGCTGGTTTAGC
CATTGCCTTAGCTGAGATGTTGGAGTTTGGAGAAGCTTATGCTAAGCAAGTTATTAAGAATGCAAAGGCATTAGCTCAGG
CTCTGTATGAGAGAGGATTTAATGTTTTATGTGAGCACAAGGACTTTACAGAAAGCCATCAAGTAATTATTGATATAGAG
AGTTCTCCAGACATAGAGTTTTCAGCAAGTGAGTTAGCTAAGATGTATGAAGAGGCAAATATCATTTTAAATAAAAACTT
ATTGCCATGGGATGATGTTAATAACTCAGACAATCCAAGTGGTATTAGGTTAGGAACACAAGAATGTACAAGATTAGGAA
TGAAAGAAAAAGAGATGGAAGAAATAGCTGAATTTATGAAAAGGATAGCTATTGATAAAGAAAAACCAGAGAAAGTTAGA
GAGGATGTTAAAGAATTTGCTAAGGAGTACAGCACTATTCATTACAGTTTTGATGAGGGAGATGGATTTAAATATCTAAG
ATTCTATTAA

Upstream 100 bases:

>100_bases
AATTAATTTTGAAGACAGTATAGTTTAAAGATATTTTTAAGTATTAATACGGTATATTTATTGTTTCTATTTCTATTAAA
CGTTAATAAGAGGGATAAAA

Downstream 100 bases:

>100_bases
TATGCTAAAATAATTTATTTTTTGCGTCATTATATATTTATATGAGTTCGTAGATATTATATTTTAGAATTTTATTATTT
GGGTTTTAGGATTTTTGATT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MEYSDVPKFIRDVSIKQHEWMRESIKLIASENITSLAVREACATDFMHRYAEGLPGKRLYQGCKYIDEVETLCIELSKEL
FKAEHANVQPTSGVVANLAVFFAETKPGDKLMALSVPDGGHISHWKVSAAGIRGLKVINHPFDPEEMNIDADAMVKKILE
EKPKLILFGGSLFPFPHPVADAYEAAQEVGAKIAYDGAHVLGLIAGKQFQDPLREGAEYLMGSTHKTFFGPQGGVILTTK
ENADKIDSHVFPGVVSNHHLHHKAGLAIALAEMLEFGEAYAKQVIKNAKALAQALYERGFNVLCEHKDFTESHQVIIDIE
SSPDIEFSASELAKMYEEANIILNKNLLPWDDVNNSDNPSGIRLGTQECTRLGMKEKEMEEIAEFMKRIAIDKEKPEKVR
EDVKEFAKEYSTIHYSFDEGDGFKYLRFY

Sequences:

>Translated_429_residues
MEYSDVPKFIRDVSIKQHEWMRESIKLIASENITSLAVREACATDFMHRYAEGLPGKRLYQGCKYIDEVETLCIELSKEL
FKAEHANVQPTSGVVANLAVFFAETKPGDKLMALSVPDGGHISHWKVSAAGIRGLKVINHPFDPEEMNIDADAMVKKILE
EKPKLILFGGSLFPFPHPVADAYEAAQEVGAKIAYDGAHVLGLIAGKQFQDPLREGAEYLMGSTHKTFFGPQGGVILTTK
ENADKIDSHVFPGVVSNHHLHHKAGLAIALAEMLEFGEAYAKQVIKNAKALAQALYERGFNVLCEHKDFTESHQVIIDIE
SSPDIEFSASELAKMYEEANIILNKNLLPWDDVNNSDNPSGIRLGTQECTRLGMKEKEMEEIAEFMKRIAIDKEKPEKVR
EDVKEFAKEYSTIHYSFDEGDGFKYLRFY
>Mature_429_residues
MEYSDVPKFIRDVSIKQHEWMRESIKLIASENITSLAVREACATDFMHRYAEGLPGKRLYQGCKYIDEVETLCIELSKEL
FKAEHANVQPTSGVVANLAVFFAETKPGDKLMALSVPDGGHISHWKVSAAGIRGLKVINHPFDPEEMNIDADAMVKKILE
EKPKLILFGGSLFPFPHPVADAYEAAQEVGAKIAYDGAHVLGLIAGKQFQDPLREGAEYLMGSTHKTFFGPQGGVILTTK
ENADKIDSHVFPGVVSNHHLHHKAGLAIALAEMLEFGEAYAKQVIKNAKALAQALYERGFNVLCEHKDFTESHQVIIDIE
SSPDIEFSASELAKMYEEANIILNKNLLPWDDVNNSDNPSGIRLGTQECTRLGMKEKEMEEIAEFMKRIAIDKEKPEKVR
EDVKEFAKEYSTIHYSFDEGDGFKYLRFY

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI22547186, Length=436, Percent_Identity=32.7981651376147, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI19923315, Length=414, Percent_Identity=31.6425120772947, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI261862352, Length=412, Percent_Identity=31.7961165048544, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI261862350, Length=412, Percent_Identity=31.7961165048544, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI261862348, Length=412, Percent_Identity=31.7961165048544, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI261862346, Length=406, Percent_Identity=32.0197044334975, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI22547189, Length=421, Percent_Identity=31.353919239905, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1788902, Length=390, Percent_Identity=38.4615384615385, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI25144732, Length=421, Percent_Identity=32.3040380047506, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI25144729, Length=421, Percent_Identity=32.3040380047506, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323087, Length=429, Percent_Identity=32.8671328671329, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=30.9226932668329, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24640005, Length=397, Percent_Identity=36.272040302267, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI221329721, Length=397, Percent_Identity=36.272040302267, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_METJA (Q58992)

Other databases:

- EMBL:   L77117
- PIR:   D64499
- RefSeq:   NP_248606.1
- ProteinModelPortal:   Q58992
- GeneID:   1452505
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1597
- NMPDR:   fig|243232.1.peg.1643
- TIGR:   MJ1597
- HOGENOM:   HBG301263
- OMA:   PEEMNID
- ProtClustDB:   PRK00011
- BioCyc:   MJAN243232:MJ_1597-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_A
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 48084; Mature: 48084

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSDVPKFIRDVSIKQHEWMRESIKLIASENITSLAVREACATDFMHRYAEGLPGKRLY
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QGCKYIDEVETLCIELSKELFKAEHANVQPTSGVVANLAVFFAETKPGDKLMALSVPDGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCEEEEEECCCCC
HISHWKVSAAGIRGLKVINHPFDPEEMNIDADAMVKKILEEKPKLILFGGSLFPFPHPVA
CCCEEEEECCCCCCHHHHCCCCCCHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
DAYEAAQEVGAKIAYDGAHVLGLIAGKQFQDPLREGAEYLMGSTHKTFFGPQGGVILTTK
HHHHHHHHHCCEEEECCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEC
ENADKIDSHVFPGVVSNHHLHHKAGLAIALAEMLEFGEAYAKQVIKNAKALAQALYERGF
CCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVLCEHKDFTESHQVIIDIESSPDIEFSASELAKMYEEANIILNKNLLPWDDVNNSDNPS
CEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCC
GIRLGTQECTRLGMKEKEMEEIAEFMKRIAIDKEKPEKVREDVKEFAKEYSTIHYSFDEG
CEEECHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCC
DGFKYLRFY
CCEEEEEEC
>Mature Secondary Structure
MEYSDVPKFIRDVSIKQHEWMRESIKLIASENITSLAVREACATDFMHRYAEGLPGKRLY
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QGCKYIDEVETLCIELSKELFKAEHANVQPTSGVVANLAVFFAETKPGDKLMALSVPDGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCEEEEEECCCCC
HISHWKVSAAGIRGLKVINHPFDPEEMNIDADAMVKKILEEKPKLILFGGSLFPFPHPVA
CCCEEEEECCCCCCHHHHCCCCCCHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
DAYEAAQEVGAKIAYDGAHVLGLIAGKQFQDPLREGAEYLMGSTHKTFFGPQGGVILTTK
HHHHHHHHHCCEEEECCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEC
ENADKIDSHVFPGVVSNHHLHHKAGLAIALAEMLEFGEAYAKQVIKNAKALAQALYERGF
CCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVLCEHKDFTESHQVIIDIESSPDIEFSASELAKMYEEANIILNKNLLPWDDVNNSDNPS
CEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCC
GIRLGTQECTRLGMKEKEMEEIAEFMKRIAIDKEKPEKVREDVKEFAKEYSTIHYSFDEG
CEEECHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCC
DGFKYLRFY
CCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087