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The map label for this gene is ligT [C]

Identifier: 15669763

GI number: 15669763

Start: 1544069

End: 1544590

Strand: Reverse

Name: ligT [C]

Synonym: MJ1568

Alternate gene names: 15669763

Gene position: 1544590-1544069 (Counterclockwise)

Preceding gene: 15669768

Following gene: 15669762

Centisome position: 92.77

GC content: 27.2

Gene sequence:

>522_bases
ATGAGGCTGTTTTTGGCTATAGACATCCCAGAAAATATAAAGGAAGAGATAGCCAAATTTCAAGAACAGTTTAAAATGAA
AGGGATAAAGTTAGTTGAGAAAGAGAACTTACATATAACCGTTAAATTCTTAGGAGAAGTTGATGAAGAAAAATTAAAAG
AAATATTGAATTTAGATTTATCAATTCAGCCAATAAAAATAAAGCTAAAATATATTGGAACATTCCCAAACTCTAACTAT
ATAAGGGTTATATGGATTGGAGCTTACAATAATAATCTTGTAGAAATCTTTAAAGAAATTGATGAAAAACTATCAAACTT
AGGATTTAAAAAAGAAAGAGAGTATGTTCCTCACTTAACAATTGGTAGAGTTAAATTCATTGATAACAAGAAAAAATTAA
AAGATAGAATTGAAAAATATAAAGATGTAGATTTTGGAGAGTTTGAAGCAAAACACATAAAGCTTTATAAATCAACTTTA
ACTCCAAACGGTCCTATATATGAGGTTATAAAAGAGTGGTAG

Upstream 100 bases:

>100_bases
ACTTAAACAAAAATAAAAATAAGTTGATATGTAAAAAATGTAATAGGGTTTATGATATAGTTGAAGGTATCCCTATACTG
CTAAGATACTGAGGGATAGG

Downstream 100 bases:

>100_bases
CAATGAATGAGAATCAGCAGAAGTTACATTATATAATAAACCTATTGACAAATGGAAATAAAAAGAGATGGGTTAAACAG
ACAGTTTTATTTGCTCTTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MRLFLAIDIPENIKEEIAKFQEQFKMKGIKLVEKENLHITVKFLGEVDEEKLKEILNLDLSIQPIKIKLKYIGTFPNSNY
IRVIWIGAYNNNLVEIFKEIDEKLSNLGFKKEREYVPHLTIGRVKFIDNKKKLKDRIEKYKDVDFGEFEAKHIKLYKSTL
TPNGPIYEVIKEW

Sequences:

>Translated_173_residues
MRLFLAIDIPENIKEEIAKFQEQFKMKGIKLVEKENLHITVKFLGEVDEEKLKEILNLDLSIQPIKIKLKYIGTFPNSNY
IRVIWIGAYNNNLVEIFKEIDEKLSNLGFKKEREYVPHLTIGRVKFIDNKKKLKDRIEKYKDVDFGEFEAKHIKLYKSTL
TPNGPIYEVIKEW
>Mature_173_residues
MRLFLAIDIPENIKEEIAKFQEQFKMKGIKLVEKENLHITVKFLGEVDEEKLKEILNLDLSIQPIKIKLKYIGTFPNSNY
IRVIWIGAYNNNLVEIFKEIDEKLSNLGFKKEREYVPHLTIGRVKFIDNKKKLKDRIEKYKDVDFGEFEAKHIKLYKSTL
TPNGPIYEVIKEW

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1568_METJA (Q58963)

Other databases:

- EMBL:   L77117
- PIR:   G64495
- RefSeq:   NP_248576.1
- ProteinModelPortal:   Q58963
- SMR:   Q58963
- GeneID:   1452476
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1568
- NMPDR:   fig|243232.1.peg.1613
- TIGR:   MJ1568
- HOGENOM:   HBG699408
- OMA:   KFVEREN
- ProtClustDB:   CLSK876623
- BioCyc:   MJAN243232:MJ_1568-MONOMER
- GO:   GO:0005622
- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097
- TIGRFAMs:   TIGR02258

Pfam domain/function: PF02834 2_5_RNA_ligase; SSF55144 Cyc_nuc_Pdiester

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20545; Mature: 20545

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLAIDIPENIKEEIAKFQEQFKMKGIKLVEKENLHITVKFLGEVDEEKLKEILNLDL
CEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCHHHHHHHHCCCC
SIQPIKIKLKYIGTFPNSNYIRVIWIGAYNNNLVEIFKEIDEKLSNLGFKKEREYVPHLT
EEEEEEEEEEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHCCCCCEE
IGRVKFIDNKKKLKDRIEKYKDVDFGEFEAKHIKLYKSTLTPNGPIYEVIKEW
ECEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHEEEEHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MRLFLAIDIPENIKEEIAKFQEQFKMKGIKLVEKENLHITVKFLGEVDEEKLKEILNLDL
CEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCHHHHHHHHCCCC
SIQPIKIKLKYIGTFPNSNYIRVIWIGAYNNNLVEIFKEIDEKLSNLGFKKEREYVPHLT
EEEEEEEEEEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHCCCCCEE
IGRVKFIDNKKKLKDRIEKYKDVDFGEFEAKHIKLYKSTLTPNGPIYEVIKEW
ECEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHEEEEHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087