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The map label for this gene is yafJ [C]

Identifier: 15669709

GI number: 15669709

Start: 1490079

End: 1491146

Strand: Direct

Name: yafJ [C]

Synonym: MJ1515

Alternate gene names: 15669709

Gene position: 1490079-1491146 (Clockwise)

Preceding gene: 15669708

Following gene: 15669710

Centisome position: 89.5

GC content: 33.05

Gene sequence:

>1068_bases
ATGTGTGAGTTGCTTGGAATTTGCTTTAATAAGAAAGTTAATGTTGAGTTATCATTAAATAGCTTTAAGCATAGGAGTGA
AGATCATCCAAATGGTTGGGGGATTGCATTTTATCCAGATGGGTTTGTTAGGGTAATAAAAGAGCCGATTAAAATGAATG
AAGCTTTATTAGCTGAATGTGTCAGATGGACTAAGATAAAATCAAATATATTCATTGCCCACATAAGAAAGGCAAGTGCT
GGAAGTGAATCTTACGTAAATACCCATCCATTTGTTAGAAAATTAGAAGATAAAGAAATAGCATTTGCTCACAATGGAAC
TCTACTTGGATATGAGGATTTAGAGCTTGATGGCTATTATCCAATTGGAGAGACGGATTCGGAGTATGTATTCTGCTATT
TATTATCTCAAATTGAAAAGAGGGAGATTGAGTGGAATAAAGAGGGTTTTGATGAGATGTTGGATATACTGCTTGATATT
AATTACTATGGAGCTTTTAATTGCTTATTCTCCGATGGAGAGTATTTATTTGCCTATAAAGATTATAGAGGGAGGAGAGA
GTTGCATTTCCTAAAGAGAAAGCCACCTTATGGAAGAATTAGATTGGAGGATGAGGATTATATTATAAACTTAGGAGATG
TTAAAAGTGTGAGAGAAGAAGGGTTTATTATAGCTACAAATCCTCTAACTAATGAAGATTGGAAGTCTTTTGAAAATGGA
GAACTTATGGTGTTTAAAAATGGGGAAATGATTTACTCTAATAAGAGATTGACAGATTTGGAATTGAAAATCTTAAAAAT
CCTTAGAGAAAGTCCCCATAGAGTCAGTTTAAAGAAAATAATTGAAAATCTTGAGTATTTATCCAGAAATATAAGACATG
ATGCTTCCGTTGTGAGAATTGGGATTAGAAGTTTGTTGGATAAGGGATATATCAAGCAAGATAGTAGAGACACTGTCAAT
TGGGATGATTTAGAGGCAACATTTTATACTAAACCAGAAAAGAGGGTAGAGATTGATAAAAGGCTTAAAGGATTTAGATG
GAGAAGAAATAGGATAATTATGAGTTAA

Upstream 100 bases:

>100_bases
TAGATTCTGGAAAGGAGATAGAGGCTTGGCTTTATTTTTATCCAGAATCTTGTGGAATTCTGGTAGAATCTGGAGATTAT
AAGGATTATAGGGGATAAGC

Downstream 100 bases:

>100_bases
TTTAATAATAGAATTGAGGTGATGGCGATGAATGAAATAGAACTAATGCAAATTAAAGATTTTGTAAAAGACATGGATAA
AAATCAACGAATTGTGTATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFYPDGFVRVIKEPIKMNEALLAECVRWTKIKSNIFIAHIRKASA
GSESYVNTHPFVRKLEDKEIAFAHNGTLLGYEDLELDGYYPIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDI
NYYGAFNCLFSDGEYLFAYKDYRGRRELHFLKRKPPYGRIRLEDEDYIINLGDVKSVREEGFIIATNPLTNEDWKSFENG
ELMVFKNGEMIYSNKRLTDLELKILKILRESPHRVSLKKIIENLEYLSRNIRHDASVVRIGIRSLLDKGYIKQDSRDTVN
WDDLEATFYTKPEKRVEIDKRLKGFRWRRNRIIMS

Sequences:

>Translated_355_residues
MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFYPDGFVRVIKEPIKMNEALLAECVRWTKIKSNIFIAHIRKASA
GSESYVNTHPFVRKLEDKEIAFAHNGTLLGYEDLELDGYYPIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDI
NYYGAFNCLFSDGEYLFAYKDYRGRRELHFLKRKPPYGRIRLEDEDYIINLGDVKSVREEGFIIATNPLTNEDWKSFENG
ELMVFKNGEMIYSNKRLTDLELKILKILRESPHRVSLKKIIENLEYLSRNIRHDASVVRIGIRSLLDKGYIKQDSRDTVN
WDDLEATFYTKPEKRVEIDKRLKGFRWRRNRIIMS
>Mature_355_residues
MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFYPDGFVRVIKEPIKMNEALLAECVRWTKIKSNIFIAHIRKASA
GSESYVNTHPFVRKLEDKEIAFAHNGTLLGYEDLELDGYYPIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDI
NYYGAFNCLFSDGEYLFAYKDYRGRRELHFLKRKPPYGRIRLEDEDYIINLGDVKSVREEGFIIATNPLTNEDWKSFENG
ELMVFKNGEMIYSNKRLTDLELKILKILRESPHRVSLKKIIENLEYLSRNIRHDASVVRIGIRSLLDKGYIKQDSRDTVN
WDDLEATFYTKPEKRVEIDKRLKGFRWRRNRIIMS

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Escherichia coli, GI1786417, Length=258, Percent_Identity=36.4341085271318, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1515_METJA (Q58910)

Other databases:

- EMBL:   L77117
- PIR:   B64489
- RefSeq:   NP_248522.1
- ProteinModelPortal:   Q58910
- SMR:   Q58910
- GeneID:   1452422
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1515
- NMPDR:   fig|243232.1.peg.1559
- TIGR:   MJ1515
- OMA:   CNTPTDI
- ProtClustDB:   CLSK2750004
- BioCyc:   MJAN243232:MJ_1515-MONOMER
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR11907

Pfam domain/function: PF00310 GATase_2

EC number: NA

Molecular weight: Translated: 41888; Mature: 41888

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFYPDGFVRVIKEPIKMNEALLAEC
CCCEEEEEECCEEEEEEEECHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHH
VRWTKIKSNIFIAHIRKASAGSESYVNTHPFVRKLEDKEIAFAHNGTLLGYEDLELDGYY
HHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEECCEEEEEECCEECCEE
PIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDINYYGAFNCLFSDGEYLFAYK
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCEEEEEC
DYRGRRELHFLKRKPPYGRIRLEDEDYIINLGDVKSVREEGFIIATNPLTNEDWKSFENG
CCCCCCHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHCCEEEEECCCCCHHHHCCCCC
ELMVFKNGEMIYSNKRLTDLELKILKILRESPHRVSLKKIIENLEYLSRNIRHDASVVRI
CEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
GIRSLLDKGYIKQDSRDTVNWDDLEATFYTKPEKRVEIDKRLKGFRWRRNRIIMS
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCEEECC
>Mature Secondary Structure
MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFYPDGFVRVIKEPIKMNEALLAEC
CCCEEEEEECCEEEEEEEECHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHH
VRWTKIKSNIFIAHIRKASAGSESYVNTHPFVRKLEDKEIAFAHNGTLLGYEDLELDGYY
HHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEECCEEEEEECCEECCEE
PIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDINYYGAFNCLFSDGEYLFAYK
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCEEEEEC
DYRGRRELHFLKRKPPYGRIRLEDEDYIINLGDVKSVREEGFIIATNPLTNEDWKSFENG
CCCCCCHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHCCEEEEECCCCCHHHHCCCCC
ELMVFKNGEMIYSNKRLTDLELKILKILRESPHRVSLKKIIENLEYLSRNIRHDASVVRI
CEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
GIRSLLDKGYIKQDSRDTVNWDDLEATFYTKPEKRVEIDKRLKGFRWRRNRIIMS
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087