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The map label for this gene is cobB

Identifier: 15669612

GI number: 15669612

Start: 1386001

End: 1387332

Strand: Reverse

Name: cobB

Synonym: MJ1421

Alternate gene names: 15669612

Gene position: 1387332-1386001 (Counterclockwise)

Preceding gene: 15669613

Following gene: 15669610

Centisome position: 83.32

GC content: 32.43

Gene sequence:

>1332_bases
ATGATTATGAAAAGAGTTGTGATTGCCGGAACATCAAGTGAAGTTGGAAAGACAGTTATCTCTACTGGAATTATGAAGGC
ATTATCAAAAAAATATAACGTTCAAGGCTATAAAGTTGGGCCTGACTATATAGACCCAACATATCACACGATAGCCACTG
GAAATAAATCAAGGAATTTAGATTCTTTTTTTATGAATAAAGAACAAATAAAATATCTTTTTCAAAAACATTCAAAAGAT
AAGGATATAAGTGTTATTGAGGGAGTTAGAGGGCTTTATGAGGGAATATCTGCAATAGATGATATTGGAAGCACAGCAAG
CGTTGCCAAGGCTTTAGATAGCCCTATAATCCTGCTTGTGAATGCAAAGAGCTTAACAAGAAGTGCAATAGCAATAATAA
AAGGTTTTATGAGTTTTGATAATGTGAAAATTAAAGGAGTTATTTTCAATTTTGTTAGAAGTGAAAACCACATAAAAAAA
TTAAAAGATGCAATGAGTTATTATCTTCCAGATATTGAAATAATTGGCTTTATCCCAAGGAATGAAGATTTTAAAGTTGA
AGGAAGGCATCTTGGTTTAGTCCCTACTCCAGAAAACTTAAAGGAGATAGAGAGTAAGATAGTGTTATGGGGGGAGTTGG
TTGAAAAATATTTGGATTTAGATAAGATTGTGGAGATAGCTGATGAGGATTTTGAAGAGGTTGATGATGTGTTTTTATGG
GAGGTTAATGAAAATTACAAAAAAATAGCTGTTGCCTATGATAAGGCATTTAATTTTTATTATTGGGATAACTTTGAAGC
TTTAAAAGAAAATAAAGCTAAGATAGAATTTTTCAGCCCATTAAAAGATAGTGAAGTTCCAGATGCAGATATTTTGTATA
TAGGAGGAGGTTATCCAGAGCTGTTTAAAGAAGAATTAAGCAGAAATAAAGAGATGATTGAAAGCATTAAAGAGTTTGAC
GGCTATATCTATGGAGAATGTGGGGGCTTGATGTATATAACAAAATCGATTGATAATGTTCCAATGGTTGGTTTATTAAA
CTGCTCAGCTGTTATGACAAAGCACGTTCAAGGACTTAGCTATGTTAAAGCTGAGTTTTTAGAGGATTGTTTAATTGGAA
GAAAGGGATTAAAGTTTAAAGGGCATGAGTTCCATTACTCAAAGCTTGTCAATATAAAAGAGGAGAGATTTGCCTATAAA
ATAGAAAGGGGGAGAGGAATTATCAATAACTTAGATGGGATTTTTAATGGTAAAGTTTTGGCTGGTTATTTACACAATCA
TGCTGTAGCTAATCCTTATTTTGCTTCATCTATGGTTAATTTTGGTGAGTAA

Upstream 100 bases:

>100_bases
AAAAAATGTATATGGCAATGTTTATAATTCACAACGTATAAACCTTTTTTAACATCCTATCATATTATGAAAAGGTTATT
TTACACATAAAAAGTAGGAG

Downstream 100 bases:

>100_bases
ATAGAAGATAAGAATGAAAGAAAAATCTCATATGAGATTCCTGAAAAAATTTCCATTTTTGATTTTAGAAATTATTTCAT
GGATTTTTGAGTTATTTTCA

Product: cobyrinic acid a,c-diamide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIMKRVVIAGTSSEVGKTVISTGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNKSRNLDSFFMNKEQIKYLFQKHSKD
KDISVIEGVRGLYEGISAIDDIGSTASVAKALDSPIILLVNAKSLTRSAIAIIKGFMSFDNVKIKGVIFNFVRSENHIKK
LKDAMSYYLPDIEIIGFIPRNEDFKVEGRHLGLVPTPENLKEIESKIVLWGELVEKYLDLDKIVEIADEDFEEVDDVFLW
EVNENYKKIAVAYDKAFNFYYWDNFEALKENKAKIEFFSPLKDSEVPDADILYIGGGYPELFKEELSRNKEMIESIKEFD
GYIYGECGGLMYITKSIDNVPMVGLLNCSAVMTKHVQGLSYVKAEFLEDCLIGRKGLKFKGHEFHYSKLVNIKEERFAYK
IERGRGIINNLDGIFNGKVLAGYLHNHAVANPYFASSMVNFGE

Sequences:

>Translated_443_residues
MIMKRVVIAGTSSEVGKTVISTGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNKSRNLDSFFMNKEQIKYLFQKHSKD
KDISVIEGVRGLYEGISAIDDIGSTASVAKALDSPIILLVNAKSLTRSAIAIIKGFMSFDNVKIKGVIFNFVRSENHIKK
LKDAMSYYLPDIEIIGFIPRNEDFKVEGRHLGLVPTPENLKEIESKIVLWGELVEKYLDLDKIVEIADEDFEEVDDVFLW
EVNENYKKIAVAYDKAFNFYYWDNFEALKENKAKIEFFSPLKDSEVPDADILYIGGGYPELFKEELSRNKEMIESIKEFD
GYIYGECGGLMYITKSIDNVPMVGLLNCSAVMTKHVQGLSYVKAEFLEDCLIGRKGLKFKGHEFHYSKLVNIKEERFAYK
IERGRGIINNLDGIFNGKVLAGYLHNHAVANPYFASSMVNFGE
>Mature_443_residues
MIMKRVVIAGTSSEVGKTVISTGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNKSRNLDSFFMNKEQIKYLFQKHSKD
KDISVIEGVRGLYEGISAIDDIGSTASVAKALDSPIILLVNAKSLTRSAIAIIKGFMSFDNVKIKGVIFNFVRSENHIKK
LKDAMSYYLPDIEIIGFIPRNEDFKVEGRHLGLVPTPENLKEIESKIVLWGELVEKYLDLDKIVEIADEDFEEVDDVFLW
EVNENYKKIAVAYDKAFNFYYWDNFEALKENKAKIEFFSPLKDSEVPDADILYIGGGYPELFKEELSRNKEMIESIKEFD
GYIYGECGGLMYITKSIDNVPMVGLLNCSAVMTKHVQGLSYVKAEFLEDCLIGRKGLKFKGHEFHYSKLVNIKEERFAYK
IERGRGIINNLDGIFNGKVLAGYLHNHAVANPYFASSMVNFGE

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_METJA (Q58816)

Other databases:

- EMBL:   L77117
- PIR:   D64477
- RefSeq:   NP_248425.1
- ProteinModelPortal:   Q58816
- GeneID:   1452325
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1421
- NMPDR:   fig|243232.1.peg.1462
- TIGR:   MJ1421
- HOGENOM:   HBG634910
- ProtClustDB:   PRK01077
- BioCyc:   MJAN243232:MJ_1421-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: NA

Molecular weight: Translated: 50206; Mature: 50206

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 327-327 ACT_SITE 425-425

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKRVVIAGTSSEVGKTVISTGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNKSRNL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCC
DSFFMNKEQIKYLFQKHSKDKDISVIEGVRGLYEGISAIDDIGSTASVAKALDSPIILLV
HHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEE
NAKSLTRSAIAIIKGFMSFDNVKIKGVIFNFVRSENHIKKLKDAMSYYLPDIEIIGFIPR
ECHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEEECC
NEDFKVEGRHLGLVPTPENLKEIESKIVLWGELVEKYLDLDKIVEIADEDFEEVDDVFLW
CCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHCCEEEE
EVNENYKKIAVAYDKAFNFYYWDNFEALKENKAKIEFFSPLKDSEVPDADILYIGGGYPE
EECCCCEEEEEEEECCEEEEEECCHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCHH
LFKEELSRNKEMIESIKEFDGYIYGECGGLMYITKSIDNVPMVGLLNCSAVMTKHVQGLS
HHHHHHHCCHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHH
YVKAEFLEDCLIGRKGLKFKGHEFHYSKLVNIKEERFAYKIERGRGIINNLDGIFNGKVL
HHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCEE
AGYLHNHAVANPYFASSMVNFGE
EEHHHCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MIMKRVVIAGTSSEVGKTVISTGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNKSRNL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCC
DSFFMNKEQIKYLFQKHSKDKDISVIEGVRGLYEGISAIDDIGSTASVAKALDSPIILLV
HHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEE
NAKSLTRSAIAIIKGFMSFDNVKIKGVIFNFVRSENHIKKLKDAMSYYLPDIEIIGFIPR
ECHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEEECC
NEDFKVEGRHLGLVPTPENLKEIESKIVLWGELVEKYLDLDKIVEIADEDFEEVDDVFLW
CCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHCCEEEE
EVNENYKKIAVAYDKAFNFYYWDNFEALKENKAKIEFFSPLKDSEVPDADILYIGGGYPE
EECCCCEEEEEEEECCEEEEEECCHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCHH
LFKEELSRNKEMIESIKEFDGYIYGECGGLMYITKSIDNVPMVGLLNCSAVMTKHVQGLS
HHHHHHHCCHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHH
YVKAEFLEDCLIGRKGLKFKGHEFHYSKLVNIKEERFAYKIERGRGIINNLDGIFNGKVL
HHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCEE
AGYLHNHAVANPYFASSMVNFGE
EEHHHCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087