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The map label for this gene is hyfI [C]

Identifier: 15669553

GI number: 15669553

Start: 1312335

End: 1312790

Strand: Reverse

Name: hyfI [C]

Synonym: MJ1363

Alternate gene names: 15669553

Gene position: 1312790-1312335 (Counterclockwise)

Preceding gene: 15669556

Following gene: 15669552

Centisome position: 78.85

GC content: 36.18

Gene sequence:

>456_bases
GTGACAGTGATGATTAAAAAAATCGCAAGGAAGAAGTGTATTCATGTAATGCTTGTTTATACTGGTGGATGTAACGCTTG
TGATATTGAAGTTGTTAATGCTATATTCTCTCCATTTTATGATGCTGAGCAGTATAATGTTTTTTTAACATTTAATCCAA
GAGAGGCAGATATTTTAGTTGTTACTGGTTGTGTTACTAAAGTTGTTGCAGAATCATTAAGAAAAATTTATGAGAAGATT
CCAGAACCAAAGGCAGTTGTTGCTGTAGGAGCTTGCGCATTGATGGGAGGAGTTTATAAAAACATTGGAGGAGATTTAGG
AACTTCAGATTTTGTTGCAGGACCTGTTGAAAACATTATTCCAGTTGATGTTAAAGTGCCTGGCTGTGCCCCAAGACCAG
AGGATATTATTGCTGGGATAGTTAAAGCTCTACCTAAGGTTATCGAAGGAAAATGA

Upstream 100 bases:

>100_bases
ATTAAAAAATCTATTATGCATATTAAAATTCTCAATAAAGCATAATCTATTTATATTTTATACATCACTATTTGTCATTA
ATGATAATGATAAATTACTG

Downstream 100 bases:

>100_bases
GGTTTTTATAAAATTTTATGAGTGAGAATGATTTATGTTTGTAAAATTTCTTTAGTGAGGGATAGGTTATGATTGATGAG
CTAATATCCATAATTGGCAT

Product: formate hydrogenlyase subunit 7

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MTVMIKKIARKKCIHVMLVYTGGCNACDIEVVNAIFSPFYDAEQYNVFLTFNPREADILVVTGCVTKVVAESLRKIYEKI
PEPKAVVAVGACALMGGVYKNIGGDLGTSDFVAGPVENIIPVDVKVPGCAPRPEDIIAGIVKALPKVIEGK

Sequences:

>Translated_151_residues
MTVMIKKIARKKCIHVMLVYTGGCNACDIEVVNAIFSPFYDAEQYNVFLTFNPREADILVVTGCVTKVVAESLRKIYEKI
PEPKAVVAVGACALMGGVYKNIGGDLGTSDFVAGPVENIIPVDVKVPGCAPRPEDIIAGIVKALPKVIEGK
>Mature_150_residues
TVMIKKIARKKCIHVMLVYTGGCNACDIEVVNAIFSPFYDAEQYNVFLTFNPREADILVVTGCVTKVVAESLRKIYEKIP
EPKAVVAVGACALMGGVYKNIGGDLGTSDFVAGPVENIIPVDVKVPGCAPRPEDIIAGIVKALPKVIEGK

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=139, Percent_Identity=33.8129496402878, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1788834, Length=134, Percent_Identity=38.8059701492537, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1789074, Length=141, Percent_Identity=36.8794326241135, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1788624, Length=124, Percent_Identity=37.0967741935484, Blast_Score=77, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17509685, Length=127, Percent_Identity=36.2204724409449, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651058, Length=127, Percent_Identity=38.5826771653543, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859983, Length=131, Percent_Identity=37.4045801526718, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24642371, Length=131, Percent_Identity=37.4045801526718, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1363_METJA (Q58758)

Other databases:

- EMBL:   L77117
- PIR:   B64470
- RefSeq:   NP_248366.1
- ProteinModelPortal:   Q58758
- SMR:   Q58758
- GeneID:   1452265
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1363
- NMPDR:   fig|243232.1.peg.1403
- TIGR:   MJ1363
- HOGENOM:   HBG553221
- OMA:   LVYTGGC
- ProtClustDB:   CLSK876547
- BioCyc:   MJAN243232:MJ_1363-MONOMER
- InterPro:   IPR006137
- InterPro:   IPR014406
- PANTHER:   PTHR11995

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.-.-.- [C]

Molecular weight: Translated: 16168; Mature: 16037

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMIKKIARKKCIHVMLVYTGGCNACDIEVVNAIFSPFYDAEQYNVFLTFNPREADILV
CCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEE
VTGCVTKVVAESLRKIYEKIPEPKAVVAVGACALMGGVYKNIGGDLGTSDFVAGPVENII
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
PVDVKVPGCAPRPEDIIAGIVKALPKVIEGK
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVMIKKIARKKCIHVMLVYTGGCNACDIEVVNAIFSPFYDAEQYNVFLTFNPREADILV
CHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEE
VTGCVTKVVAESLRKIYEKIPEPKAVVAVGACALMGGVYKNIGGDLGTSDFVAGPVENII
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
PVDVKVPGCAPRPEDIIAGIVKALPKVIEGK
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087