Click here to switch to the map view.

The map label for this gene is nadE

Identifier: 15669543

GI number: 15669543

Start: 1301190

End: 1301969

Strand: Reverse

Name: nadE

Synonym: MJ1352

Alternate gene names: 15669543

Gene position: 1301969-1301190 (Counterclockwise)

Preceding gene: 15669547

Following gene: 15669542

Centisome position: 78.2

GC content: 34.1

Gene sequence:

>780_bases
ATGTTATGGGGTGAGAGTATGGAAGAGATAGTTAATAAGATTACAAAATTTATCAGGGAGAAGGTTGAAGAAGCCAATGC
CAATGGAGTTGTTGTTGGATTAAGTGGGGGTATTGATTCTTCTGTTACAGCTTATTTATGTGTTAAGGCACTTGGAAAAG
ATAAAGTTCTCGGCTTAATAATGCCAGAGAAGAATACAAATCCAAAAGATGTTGAACATGCAAAGATGGTTGCTGAGAAT
TTAGGAATAAAGTATATTATCTCAGATATAACAGATATCTTAAAGGCATTTGGTGCTGGAGGTTATGTCCCAACGAGAGA
GTTTGATAAGATAGCGGATGGAAATTTAAAGGCAAGGATTAGGATGTGCATCCTCTATTACTTTGCAAATAAATATAATT
TATTAGTTGCTGGAACTTCCAATAAATCTGAGATTTATGTTGGATATGGAACAAAACATGGAGACATTGCTTGTGATATA
AGACCAATAGGCAATTTATTTAAAACAGAGGTTAAAAAACTTGCTAAATATATTGGTGTTCCAAAGGAAATTATTGAAAA
ACCACCATCAGCAGGGCTTTGGGAAGGACAGACAGATGAAGAGGAGCTTGACATTAAGTATGAAACTTTAGATACGATAT
TAAAGCTTTATGAGAAGGGCAAAACTCCAGAGGAGATTCATAAAGAGACAAACATTCCATTGGAAACTATTAACTATGTG
TTTGATTTAATTAAAAAGAATGAGCATAAGAGAACTTTACCTCCAACACCAGAGATTTAA

Upstream 100 bases:

>100_bases
AGATGAGTTGTGTAAAATTCCAGAGCTTAAGGTGTGTGCTGTAAAGATTGAACGAATTTAATTTATAGAATTGTTTATAT
AATAGGAATCATATTTCCTA

Downstream 100 bases:

>100_bases
TTTTTAATTTTAGTTTAAATATTTTATTTTAGTTATTCTATTTTAAAATTAAATTATTTTATATATTGTAATATTCCAAA
TCATAAGTCTCAGACCATAA

Product: NH(3)-dependent NAD+ synthetase NadE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLWGESMEEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLIMPEKNTNPKDVEHAKMVAEN
LGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARIRMCILYYFANKYNLLVAGTSNKSEIYVGYGTKHGDIACDI
RPIGNLFKTEVKKLAKYIGVPKEIIEKPPSAGLWEGQTDEEELDIKYETLDTILKLYEKGKTPEEIHKETNIPLETINYV
FDLIKKNEHKRTLPPTPEI

Sequences:

>Translated_259_residues
MLWGESMEEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLIMPEKNTNPKDVEHAKMVAEN
LGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARIRMCILYYFANKYNLLVAGTSNKSEIYVGYGTKHGDIACDI
RPIGNLFKTEVKKLAKYIGVPKEIIEKPPSAGLWEGQTDEEELDIKYETLDTILKLYEKGKTPEEIHKETNIPLETINYV
FDLIKKNEHKRTLPPTPEI
>Mature_259_residues
MLWGESMEEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLIMPEKNTNPKDVEHAKMVAEN
LGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARIRMCILYYFANKYNLLVAGTSNKSEIYVGYGTKHGDIACDI
RPIGNLFKTEVKKLAKYIGVPKEIIEKPPSAGLWEGQTDEEELDIKYETLDTILKLYEKGKTPEEIHKETNIPLETINYV
FDLIKKNEHKRTLPPTPEI

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=245, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538756, Length=278, Percent_Identity=25.8992805755396, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_METJA (Q58747)

Other databases:

- EMBL:   L77117
- PIR:   G64468
- RefSeq:   NP_248355.1
- ProteinModelPortal:   Q58747
- SMR:   Q58747
- GeneID:   1452255
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1352
- NMPDR:   fig|243232.1.peg.1392
- TIGR:   MJ1352
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   CLSK2749980
- BioCyc:   MJAN243232:MJ_1352-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 28982; Mature: 28982

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: ACT_SITE 35-35

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWGESMEEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
MPEKNTNPKDVEHAKMVAENLGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARI
ECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHH
RMCILYYFANKYNLLVAGTSNKSEIYVGYGTKHGDIACDIRPIGNLFKTEVKKLAKYIGV
HHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHCC
PKEIIEKPPSAGLWEGQTDEEELDIKYETLDTILKLYEKGKTPEEIHKETNIPLETINYV
CHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHH
FDLIKKNEHKRTLPPTPEI
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLWGESMEEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
MPEKNTNPKDVEHAKMVAENLGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARI
ECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHH
RMCILYYFANKYNLLVAGTSNKSEIYVGYGTKHGDIACDIRPIGNLFKTEVKKLAKYIGV
HHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHCC
PKEIIEKPPSAGLWEGQTDEEELDIKYETLDTILKLYEKGKTPEEIHKETNIPLETINYV
CHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHH
FDLIKKNEHKRTLPPTPEI
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087