BacMap

Definition	Methanocaldococcus jannaschii DSM 2661 chromosome, complete genome.
Accession	NC_000909
Length	1,664,970

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The map label for this gene is pycA

Identifier: 15669414

GI number: 15669414

Start: 1171711

End: 1173216

Strand: Reverse

Name: pycA

Synonym: MJ1229

Alternate gene names: 15669414

Gene position: 1173216-1171711 (Counterclockwise)

Preceding gene: 15669415

Following gene: 15669413

Centisome position: 70.46

GC content: 36.79

Gene sequence:

>1506_bases
ATGTTTAACAAAGTTTTAATTGCAAATAGAGGGGAGATAGCGATTAGAATTATAAGAGCATGTTGGGAGTTGGGAATTAA
GACAGTTGCAGTTTATTCTGAGGCAGATAAGAGGTCTTTACATGCTACTTTGGCTGATGAAGCTTACTGTATAGGTCCTG
CTCCAGCGGCAAAGAGTTATTTAAACATTGATGCCATATTAAATGTAGCTGAGAAAGCTAAGGTTGATGCCATCCATCCA
GGATATGGATTTTTAGCTGAAAATGCTGAATTTGCAAGGGCTGTTAAAAAAGCTGGTTTTGAATTTATAGGGCCTAATCC
AGATGCTATAGAAGCAATGGGAAGTAAAATTAACGCTAAAAAAATCATGAAAAAAGCAGGAGTTCCTTTAATTCCTGGTA
GTGAGGGGGCTATTGAAGATATTGATGAAGCAATAGAAATAGCTGAAGCTATCGGTTTCCCTGTAGTTGTTAAAGCTTCA
GCTGGCGGTGGCGGAATGGGAATGAGTGTTGCATATAGCAAAGAGGAGTTAAAAGAAGTTATTGAATCTGCAAGAAACAT
TGCAAAGAGTGCATTTGGTGACCCAACAGTATTTATTGAGAAATACTTAGAAAATCCAAGACACATTGAAATCCAATTAT
TGGGAGATAAGCATGGGAATATTATTCATTTAGGAGATAGAGAGTGTTCAATACAGAGGAGACATCAGAAGTTGATTGAA
GAGGCTCCCTCACCAATAATGACTGAAGAGTTAAGAGAAAGAATGGGAGAAGCGGCAATCAAAGCAGGAAAGGCAATAAA
TTATGACAGTGCAGGAACTGTTGAGTTTTTGTATGAAAATGGCAACTTTTACTTCTTAGAGATGAATACAAGAATTCAAG
TTGAGCATACAGTTACAGAGCAAGTTACTGGAATAGATTTGGTTAAGGCGATGATTAAAATAGCTGCTGGAGAAGAATTA
ACTTTAAAGCAGGAAGATGTTAAAATAAGAGGGCATGCAATTGAGTGCAGAATTAACGCAGAAGACCCATTAAATGATTT
CGTTCCATGTCCTGGAAAGATAAAACTATATAGGTCTCCAGGGGGGCCTGGAGTTAGGATTGACAGTGGTGTCTATGGAG
GGGCTGAAATTCCTCCTTACTATGATTCAATGATAGCTAAGCTAATTACTTATGGAAATAGCAGAGAGGAGGCAATAGCA
AGAATGAAAAGAGCTTTGAGGGAGTATGTTATAATAGGCGTTAAAACAAATATTCCATTCCATAGGGCTGTTTTAGAGGA
GGAGAACTTTTTAAAAGGGAATATCTCAACTCACTATGTAGAGCAGAATATGCATAAATTAAGAGAGAAAATGGTTAAAT
ACGCATTAGAATCAAGAGATTTATACAGTGTTGTATCAGAGAAGGTATTTGAAAAGAATAAAAAGATAGCCGCCGCTGTT
GGTGGTTTAACAATGTATATATCCCAAATTATGAAAGAAAATGAAGTGAATAACAAAGAATGGTAA

Upstream 100 bases:

>100_bases
AAAATGCGGATATTCTGAATTTTATGATAAGAGTATAGTCAAGAGTAAGGATAATTTAATGAATATTTTAGATATCTTCT
TTGGATAGAGGTGAAAAATC

Downstream 100 bases:

>100_bases
CTATCTAAAATTTTATTTTTTATATGCTTAAAAGATAGAAGTTATTGAAATTTTGTTAAATTTGAATATTAAATTTAATA
GATTAATAAGATTAATAATC

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFNKVLIANRGEIAIRIIRACWELGIKTVAVYSEADKRSLHATLADEAYCIGPAPAAKSYLNIDAILNVAEKAKVDAIHP
GYGFLAENAEFARAVKKAGFEFIGPNPDAIEAMGSKINAKKIMKKAGVPLIPGSEGAIEDIDEAIEIAEAIGFPVVVKAS
AGGGGMGMSVAYSKEELKEVIESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLGDKHGNIIHLGDRECSIQRRHQKLIE
EAPSPIMTEELRERMGEAAIKAGKAINYDSAGTVEFLYENGNFYFLEMNTRIQVEHTVTEQVTGIDLVKAMIKIAAGEEL
TLKQEDVKIRGHAIECRINAEDPLNDFVPCPGKIKLYRSPGGPGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIA
RMKRALREYVIIGVKTNIPFHRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVSEKVFEKNKKIAAAV
GGLTMYISQIMKENEVNNKEW

Sequences:

>Translated_501_residues
MFNKVLIANRGEIAIRIIRACWELGIKTVAVYSEADKRSLHATLADEAYCIGPAPAAKSYLNIDAILNVAEKAKVDAIHP
GYGFLAENAEFARAVKKAGFEFIGPNPDAIEAMGSKINAKKIMKKAGVPLIPGSEGAIEDIDEAIEIAEAIGFPVVVKAS
AGGGGMGMSVAYSKEELKEVIESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLGDKHGNIIHLGDRECSIQRRHQKLIE
EAPSPIMTEELRERMGEAAIKAGKAINYDSAGTVEFLYENGNFYFLEMNTRIQVEHTVTEQVTGIDLVKAMIKIAAGEEL
TLKQEDVKIRGHAIECRINAEDPLNDFVPCPGKIKLYRSPGGPGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIA
RMKRALREYVIIGVKTNIPFHRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVSEKVFEKNKKIAAAV
GGLTMYISQIMKENEVNNKEW
>Mature_501_residues
MFNKVLIANRGEIAIRIIRACWELGIKTVAVYSEADKRSLHATLADEAYCIGPAPAAKSYLNIDAILNVAEKAKVDAIHP
GYGFLAENAEFARAVKKAGFEFIGPNPDAIEAMGSKINAKKIMKKAGVPLIPGSEGAIEDIDEAIEIAEAIGFPVVVKAS
AGGGGMGMSVAYSKEELKEVIESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLGDKHGNIIHLGDRECSIQRRHQKLIE
EAPSPIMTEELRERMGEAAIKAGKAINYDSAGTVEFLYENGNFYFLEMNTRIQVEHTVTEQVTGIDLVKAMIKIAAGEEL
TLKQEDVKIRGHAIECRINAEDPLNDFVPCPGKIKLYRSPGGPGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIA
RMKRALREYVIIGVKTNIPFHRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVSEKVFEKNKKIAAAV
GGLTMYISQIMKENEVNNKEW

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain

Homologues:

Organism=Homo sapiens, GI189095269, Length=442, Percent_Identity=54.2986425339367, Blast_Score=487, Evalue=1e-138,
Organism=Homo sapiens, GI65506442, Length=442, Percent_Identity=54.2986425339367, Blast_Score=487, Evalue=1e-138,
Organism=Homo sapiens, GI295821183, Length=442, Percent_Identity=54.2986425339367, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI116805327, Length=481, Percent_Identity=47.4012474012474, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI106049528, Length=465, Percent_Identity=47.741935483871, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI106049295, Length=465, Percent_Identity=47.741935483871, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI106049292, Length=465, Percent_Identity=47.741935483871, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI38679960, Length=501, Percent_Identity=31.7365269461078, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI38679974, Length=501, Percent_Identity=31.7365269461078, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI38679971, Length=501, Percent_Identity=31.7365269461078, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI38679977, Length=501, Percent_Identity=31.7365269461078, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI38679967, Length=501, Percent_Identity=31.7365269461078, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI134142062, Length=503, Percent_Identity=32.20675944334, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI170295797, Length=317, Percent_Identity=22.0820189274448, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI21361331, Length=317, Percent_Identity=22.0820189274448, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI169790915, Length=317, Percent_Identity=22.0820189274448, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789654, Length=451, Percent_Identity=57.649667405765, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1786216, Length=325, Percent_Identity=25.8461538461538, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567343, Length=442, Percent_Identity=52.262443438914, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17562816, Length=448, Percent_Identity=47.9910714285714, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71987519, Length=447, Percent_Identity=47.427293064877, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71997168, Length=513, Percent_Identity=30.0194931773879, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71997163, Length=513, Percent_Identity=30.0194931773879, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI133931226, Length=487, Percent_Identity=29.1581108829569, Blast_Score=217, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6319695, Length=450, Percent_Identity=47.3333333333333, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321376, Length=449, Percent_Identity=47.6614699331849, Blast_Score=405, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=44.3181818181818, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323863, Length=457, Percent_Identity=34.5733041575492, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6324343, Length=523, Percent_Identity=31.7399617590822, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=23.7942122186495, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322569, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24652212, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24652210, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24652214, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921944, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24652216, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI281363050, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24652224, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24652222, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24652220, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24652218, Length=445, Percent_Identity=50.561797752809, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651757, Length=449, Percent_Identity=45.43429844098, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651759, Length=412, Percent_Identity=44.4174757281553, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI161076409, Length=498, Percent_Identity=30.1204819277108, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24586458, Length=498, Percent_Identity=30.1204819277108, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI161076407, Length=498, Percent_Identity=30.1204819277108, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24586460, Length=498, Percent_Identity=30.1204819277108, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYCA_METJA (Q58626)

Other databases:

- EMBL:   L77117
- PIR:   D64453
- RefSeq:   NP_248224.1
- ProteinModelPortal:   Q58626
- SMR:   Q58626
- GeneID:   1452125
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1229
- NMPDR:   fig|243232.1.peg.1261
- TIGR:   MJ1229
- HOGENOM:   HBG535236
- OMA:   EAPSPIM
- ProtClustDB:   PRK08654
- BioCyc:   MJAN243232:MJ_1229-MONOMER
- BRENDA:   6.4.1.1
- GO:   GO:0006094
- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- SMART:   SM00878
- TIGRFAMs:   TIGR00514

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.4.1.1

Molecular weight: Translated: 55404; Mature: 55404

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50975 ATP_GRASP; PS50979 BC; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: ACT_SITE 291-291 BINDING 116-116 BINDING 200-200 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKVLIANRGEIAIRIIRACWELGIKTVAVYSEADKRSLHATLADEAYCIGPAPAAKSY
CCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHEEECCCCEEECCCHHHHHH
LNIDAILNVAEKAKVDAIHPGYGFLAENAEFARAVKKAGFEFIGPNPDAIEAMGSKINAK
CCHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHH
KIMKKAGVPLIPGSEGAIEDIDEAIEIAEAIGFPVVVKASAGGGGMGMSVAYSKEELKEV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECHHHHHHH
IESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLGDKHGNIIHLGDRECSIQRRHQKLIE
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHH
EAPSPIMTEELRERMGEAAIKAGKAINYDSAGTVEFLYENGNFYFLEMNTRIQVEHTVTE
HCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCEEEEEECCEEEEEEHHHH
QVTGIDLVKAMIKIAAGEELTLKQEDVKIRGHAIECRINAEDPLNDFVPCPGKIKLYRSP
HHHHHHHHHHHHHHHCCCCEEEECCCEEEECEEEEEEECCCCCCHHCCCCCCCEEEEECC
GGPGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIARMKRALREYVIIGVKTNIPF
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCH
HRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVSEKVFEKNKKIAAAV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHH
GGLTMYISQIMKENEVNNKEW
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFNKVLIANRGEIAIRIIRACWELGIKTVAVYSEADKRSLHATLADEAYCIGPAPAAKSY
CCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHEEECCCCEEECCCHHHHHH
LNIDAILNVAEKAKVDAIHPGYGFLAENAEFARAVKKAGFEFIGPNPDAIEAMGSKINAK
CCHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHH
KIMKKAGVPLIPGSEGAIEDIDEAIEIAEAIGFPVVVKASAGGGGMGMSVAYSKEELKEV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECHHHHHHH
IESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLGDKHGNIIHLGDRECSIQRRHQKLIE
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHH
EAPSPIMTEELRERMGEAAIKAGKAINYDSAGTVEFLYENGNFYFLEMNTRIQVEHTVTE
HCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCEEEEEECCEEEEEEHHHH
QVTGIDLVKAMIKIAAGEELTLKQEDVKIRGHAIECRINAEDPLNDFVPCPGKIKLYRSP
HHHHHHHHHHHHHHHCCCCEEEECCCEEEECEEEEEEECCCCCCHHCCCCCCCEEEEECC
GGPGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIARMKRALREYVIIGVKTNIPF
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCH
HRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVSEKVFEKNKKIAAAV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHH
GGLTMYISQIMKENEVNNKEW
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096