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The map label for this gene is dapF

Identifier: 15669306

GI number: 15669306

Start: 1059445

End: 1060332

Strand: Direct

Name: dapF

Synonym: MJ1119

Alternate gene names: 15669306

Gene position: 1059445-1060332 (Clockwise)

Preceding gene: 15669305

Following gene: 15669307

Centisome position: 63.63

GC content: 33.78

Gene sequence:

>888_bases
ATGGAATTTACAAAGATGCATGCCCTTGGGAATGATTACATAGTTATTAATGAATTTGACGGGGAAAAAGTTAAAGAAGA
AGAAAAAGCAGAGTTCTCAAGAAAAATTTGTAGAAGAGGTTTTTCAGTAGGTGCTGATGGAGTTATTTTCATCCAAAAGC
CAACATCTGATGAATACGATGTAAGATTTAGAATATTTAACAGTGACGGCTCTGAGGCAGAGATGTGTGGTAATGGAATT
AGATGTTTTTCAAAATACGTTTATGAGAGAATAATGAAAAAAAATCCTTTAAAAGTAGAGACAAAAGGGGGCTTAAGAGT
ATCTGAAATGGAGATAGAAGGAGATGAAGTAAAGAAGATTAAAGTTTATATGGGAGTTCCAAAGTTTAAATTAAAAGATA
TACCAATGGTTGTTGATGGTTATAAAGAGGATGATGAATTTTTGAATGGAGAGTTAAAATTAAAGAATCCATATTTACCT
AAGGTCAAGTTGAGTGTCGTTAATGTTGGAAATCCACATGCGGTTATATTTGTTGAAGATAACAACATAGATTTAGATTT
TGTTAGAGAACACTTGGATGTTATTGGGAAAGAGATAGAACACCATGAAGCATTCCCAGAGAGAATTAATGTGCATTTTG
TAAAAGTTTTGAATCCTAATGAAATTAGGATTGTTACCTGGGAGAGGGGAGCTGGATACACAACTGCATGCGGAACAGGA
ACAACAGCTTCTGTAATTATGGCTCACAAACTTGGTAAAACAAATAATAGGGTTTTAGCTCACTTAGATGGTGGAGATTT
GGAGATTGAGATTAAAGATGATGGAGTTTATATGATTGGAGATGCAGTAATGGTTTATGACGCCAAATTGATAAATATTG
GCTGGTAA

Upstream 100 bases:

>100_bases
CTTTGCAAATGTGATTAAAAACTAACTAAGGAAAGTATGCCTTTCTTTATTACAAAATGTTTTACAAAGAGCTACAAATA
ACTATTTTTGGTGTGAAAAT

Downstream 100 bases:

>100_bases
ATTATGAAAGTAGATTTAACCATTGAATATAGAGGAAAATTAATTACTCCAAATCAAATAAAACTTTTAATTGCCTTACA
TAAAACAAAATCCCAAAATG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYDVRFRIFNSDGSEAEMCGNGI
RCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKIKVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLP
KVKLSVVNVGNPHAVIFVEDNNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTG
TTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKLINIGW

Sequences:

>Translated_295_residues
MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYDVRFRIFNSDGSEAEMCGNGI
RCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKIKVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLP
KVKLSVVNVGNPHAVIFVEDNNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTG
TTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKLINIGW
>Mature_295_residues
MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYDVRFRIFNSDGSEAEMCGNGI
RCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKIKVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLP
KVKLSVVNVGNPHAVIFVEDNNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTG
TTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKLINIGW

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=290, Percent_Identity=35.5172413793103, Blast_Score=165, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_METJA (Q58519)

Other databases:

- EMBL:   L77117
- PIR:   F64439
- RefSeq:   NP_248111.1
- ProteinModelPortal:   Q58519
- SMR:   Q58519
- GeneID:   1452015
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1119
- NMPDR:   fig|243232.1.peg.1148
- TIGR:   MJ1119
- HOGENOM:   HBG399442
- OMA:   EIWERGA
- ProtClustDB:   PRK00450
- BioCyc:   MJAN243232:MJ_1119-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 33443; Mature: 33443

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYD
CCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCE
VRFRIFNSDGSEAEMCGNGIRCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKI
EEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCEEEE
KVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLPKVKLSVVNVGNPHAVIFVED
EEEECCCCEEECCCCEEECCCCCCHHHCCCEEEECCCCCCEEEEEEEECCCCEEEEEEEC
NNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEECCCC
TTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKLINIGW
CHHHEEEEECCCCCCCEEEEEECCCEEEEEEECCCEEEEECEEEEEEEEEEEECC
>Mature Secondary Structure
MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYD
CCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCE
VRFRIFNSDGSEAEMCGNGIRCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKI
EEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCEEEE
KVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLPKVKLSVVNVGNPHAVIFVED
EEEECCCCEEECCCCEEECCCCCCHHHCCCEEEECCCCCCEEEEEEEECCCCEEEEEEEC
NNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEECCCC
TTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKLINIGW
CHHHEEEEECCCCCCCEEEEEECCCEEEEEEECCCEEEEECEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087