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The map label for this gene is trpE

Identifier: 15669263

GI number: 15669263

Start: 1014198

End: 1015622

Strand: Reverse

Name: trpE

Synonym: MJ1075

Alternate gene names: 15669263

Gene position: 1015622-1014198 (Counterclockwise)

Preceding gene: 15669264

Following gene: 15669262

Centisome position: 61.0

GC content: 32.7

Gene sequence:

>1425_bases
ATGATAATTAAAAAAATAAAAATGGATGTTTGTCCATTAGATGTTTATGAGCAAATTAGGGGAGAGAATACATTTTTGTT
AGAATCAGCTGAAGGAGTTCCAAAGGTGGCAAGATACTCAATCTTAGGAAAAGCTGAAGGAAAAGTAATATTTAAAAATG
GAAAGCTGAAAGTTGAAAGCTTTACAGAATTTGGAGATAAAGCTAAAGATTTAGAAGGGAAATACGAATGTCCCTTAGAC
GCTTTAAGAGAGGTTAGAAATGAATATCTTAAATACATTGATATATCTAACATTGAGCCAATACCAAGATTTAAGGGGGG
TTTAGTTGGGTATTTAAGCTATGATATTATCAGATACTGGATAGATTTATCAAATATCAACCCAAAGCCAATAAATGATT
TAAAATTTCCAGATGCAGAGTTCTTTATTGTTAAGGACTTTATTTCATTTGATTTAAAAGAGAAAGTAATTAATTTAATA
GCAGAGGATGATGAAGGTATTAGAGAACTTGAAAGAATTATAAAAAATGCAAAAATTGGAAATAATGACAATAAAGAAGA
AAAAACTACAGAAAATAAGGACTTAAAAATAAAATCTAACATGAGCAAAGAGGAATTTATTGAGGCGGTTAAAAAAGCTA
AGGAATACATTTTTGCTGGAGATATCTTCCAAGTGGTTTTATCAAGAAGGATAGAGATAGATTTAGATAACTTAGACCAC
TTGAAAATTTACAAAAAAGTTAGAGAGATAAATCCTTCCCCATACATGTATTACTTAGATTTTGGAGACAGAAAGATTAT
AGGTTCATCACCAGAGATTTTGGTAAGGACAGATTATAAAGATAATAAAAGGCTGGTTATAACAAGACCTATAGCTGGAA
CAATTAGGAGGGGTAAGACAGAAGAAGAAGATAAAGAGTTAGAGAAAAAGCTGTTAAGTGATGAGAAAGAGAGGGCAGAG
CATGTTATGCTTGTAGATTTAGCAAGGAATGATATTGGAAAAATATCAAAATTTGGAACTGTTGAAGTTACTGATTTCAT
GATTATTGAGAAATACTCCCATGTTCAGCATATAGTAAGTAATGTTGTTGGGGAGTTAAAAGACAATTATGATTCATTCT
TAGCTGTAAAAGCTACCTTCCCAGCGGGAACTTTAAGTGGAGCACCAAAGGTCAGAGCGATGGAGATTATTGAAGAGCTT
GAAAAAACTTGGAGAGGACCTTATGGTGGGGGAGTTGGCTATTTCGGATGGGATGATTTAATGGATTTGGCTATAACAAT
CAGAACCTTTGTAATCTCGAAAAATAAGGGATATATTCAAGTTGGTGCTGGAATTGTAGCTGATTCAATCCCAGAAAATG
AATGGGAAGAGACAGAGAGAAAGGGAATGGCTAACGTTAAGACGATTGAGAGTTTATTGAAATGA

Upstream 100 bases:

>100_bases
ATGAGATTTTGTTTTATGACGTTATTAATGGGATAATTAAGCCTACATCGGTAAAGAAATGGTATGCCATAAAAGAAATT
TTGGATAAATAGGTGATTTA

Downstream 100 bases:

>100_bases
TAAGTTTAGAAATGGTTTTATAGCAAAAAAATTAAATAATATGATTTAAAGATTTGGTGAAATTATGGCAATTGCTTATG
CTAAGTTGTATGAATTAATT

Product: anthranilate synthase component I TrpE

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIIKKIKMDVCPLDVYEQIRGENTFLLESAEGVPKVARYSILGKAEGKVIFKNGKLKVESFTEFGDKAKDLEGKYECPLD
ALREVRNEYLKYIDISNIEPIPRFKGGLVGYLSYDIIRYWIDLSNINPKPINDLKFPDAEFFIVKDFISFDLKEKVINLI
AEDDEGIRELERIIKNAKIGNNDNKEEKTTENKDLKIKSNMSKEEFIEAVKKAKEYIFAGDIFQVVLSRRIEIDLDNLDH
LKIYKKVREINPSPYMYYLDFGDRKIIGSSPEILVRTDYKDNKRLVITRPIAGTIRRGKTEEEDKELEKKLLSDEKERAE
HVMLVDLARNDIGKISKFGTVEVTDFMIIEKYSHVQHIVSNVVGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEEL
EKTWRGPYGGGVGYFGWDDLMDLAITIRTFVISKNKGYIQVGAGIVADSIPENEWEETERKGMANVKTIESLLK

Sequences:

>Translated_474_residues
MIIKKIKMDVCPLDVYEQIRGENTFLLESAEGVPKVARYSILGKAEGKVIFKNGKLKVESFTEFGDKAKDLEGKYECPLD
ALREVRNEYLKYIDISNIEPIPRFKGGLVGYLSYDIIRYWIDLSNINPKPINDLKFPDAEFFIVKDFISFDLKEKVINLI
AEDDEGIRELERIIKNAKIGNNDNKEEKTTENKDLKIKSNMSKEEFIEAVKKAKEYIFAGDIFQVVLSRRIEIDLDNLDH
LKIYKKVREINPSPYMYYLDFGDRKIIGSSPEILVRTDYKDNKRLVITRPIAGTIRRGKTEEEDKELEKKLLSDEKERAE
HVMLVDLARNDIGKISKFGTVEVTDFMIIEKYSHVQHIVSNVVGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEEL
EKTWRGPYGGGVGYFGWDDLMDLAITIRTFVISKNKGYIQVGAGIVADSIPENEWEETERKGMANVKTIESLLK
>Mature_474_residues
MIIKKIKMDVCPLDVYEQIRGENTFLLESAEGVPKVARYSILGKAEGKVIFKNGKLKVESFTEFGDKAKDLEGKYECPLD
ALREVRNEYLKYIDISNIEPIPRFKGGLVGYLSYDIIRYWIDLSNINPKPINDLKFPDAEFFIVKDFISFDLKEKVINLI
AEDDEGIRELERIIKNAKIGNNDNKEEKTTENKDLKIKSNMSKEEFIEAVKKAKEYIFAGDIFQVVLSRRIEIDLDNLDH
LKIYKKVREINPSPYMYYLDFGDRKIIGSSPEILVRTDYKDNKRLVITRPIAGTIRRGKTEEEDKELEKKLLSDEKERAE
HVMLVDLARNDIGKISKFGTVEVTDFMIIEKYSHVQHIVSNVVGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEEL
EKTWRGPYGGGVGYFGWDDLMDLAITIRTFVISKNKGYIQVGAGIVADSIPENEWEETERKGMANVKTIESLLK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family

Homologues:

Organism=Escherichia coli, GI1787518, Length=411, Percent_Identity=35.2798053527981, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788114, Length=388, Percent_Identity=32.2164948453608, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1786809, Length=255, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082077, Length=271, Percent_Identity=25.0922509225092, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=484, Percent_Identity=38.2231404958678, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6324361, Length=407, Percent_Identity=26.044226044226, Blast_Score=110, Evalue=6e-25,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_METJA (Q58475)

Other databases:

- EMBL:   L77117
- PIR:   B64434
- RefSeq:   NP_248068.1
- ProteinModelPortal:   Q58475
- SMR:   Q58475
- GeneID:   1451971
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1075
- NMPDR:   fig|243232.1.peg.1105
- TIGR:   MJ1075
- HOGENOM:   HBG507440
- OMA:   ERKGMAN
- ProtClustDB:   CLSK2517684
- BioCyc:   MJAN243232:MJ_1075-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR010116
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR01820

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 54300; Mature: 54300

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKIKMDVCPLDVYEQIRGENTFLLESAEGVPKVARYSILGKAEGKVIFKNGKLKVES
CCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHEEECCCCEEEEECCCEEEEH
FTEFGDKAKDLEGKYECPLDALREVRNEYLKYIDISNIEPIPRFKGGLVGYLSYDIIRYW
HHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHH
IDLSNINPKPINDLKFPDAEFFIVKDFISFDLKEKVINLIAEDDEGIRELERIIKNAKIG
HHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
NNDNKEEKTTENKDLKIKSNMSKEEFIEAVKKAKEYIFAGDIFQVVLSRRIEIDLDNLDH
CCCCCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHH
LKIYKKVREINPSPYMYYLDFGDRKIIGSSPEILVRTDYKDNKRLVITRPIAGTIRRGKT
HHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEECCCCCCEEEEECCCHHHHHCCCC
EEEDKELEKKLLSDEKERAEHVMLVDLARNDIGKISKFGTVEVTDFMIIEKYSHVQHIVS
CHHHHHHHHHHHCCHHHHHHEEEEEEEHHCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
NVVGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEELEKTWRGPYGGGVGYFGWDDL
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
MDLAITIRTFVISKNKGYIQVGAGIVADSIPENEWEETERKGMANVKTIESLLK
HHHHHHHHHHEEECCCCEEEECCCHHCCCCCCCHHHHHHHCCCCHHHHHHHHHC
>Mature Secondary Structure
MIIKKIKMDVCPLDVYEQIRGENTFLLESAEGVPKVARYSILGKAEGKVIFKNGKLKVES
CCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHEEECCCCEEEEECCCEEEEH
FTEFGDKAKDLEGKYECPLDALREVRNEYLKYIDISNIEPIPRFKGGLVGYLSYDIIRYW
HHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHH
IDLSNINPKPINDLKFPDAEFFIVKDFISFDLKEKVINLIAEDDEGIRELERIIKNAKIG
HHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
NNDNKEEKTTENKDLKIKSNMSKEEFIEAVKKAKEYIFAGDIFQVVLSRRIEIDLDNLDH
CCCCCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHH
LKIYKKVREINPSPYMYYLDFGDRKIIGSSPEILVRTDYKDNKRLVITRPIAGTIRRGKT
HHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEECCCCCCEEEEECCCHHHHHCCCC
EEEDKELEKKLLSDEKERAEHVMLVDLARNDIGKISKFGTVEVTDFMIIEKYSHVQHIVS
CHHHHHHHHHHHCCHHHHHHEEEEEEEHHCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
NVVGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEELEKTWRGPYGGGVGYFGWDDL
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
MDLAITIRTFVISKNKGYIQVGAGIVADSIPENEWEETERKGMANVKTIESLLK
HHHHHHHHHHEEECCCCEEEECCCHHCCCCCCCHHHHHHHCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087