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The map label for this gene is rffG [C]

Identifier: 15669250

GI number: 15669250

Start: 1002063

End: 1003064

Strand: Reverse

Name: rffG [C]

Synonym: MJ1061

Alternate gene names: 15669250

Gene position: 1003064-1002063 (Counterclockwise)

Preceding gene: 15669251

Following gene: 15669242

Centisome position: 60.25

GC content: 28.64

Gene sequence:

>1002_bases
ATGTTTCAAGATATATCAAATTTTTATAAAGATAAAACTATTCTCGTTACAGGAGGAACTGGCTCAATAGGTAAAGAAAT
AGTAAAAACATTATTAAAATTTAATCCAAAAACAATTAGAGTATTAGATATAAATGAAACTGCATTGTTTGAATTAGAAC
ATGAGCTAAATTCAGAGAAAATTAGATGTTTTATTGGGGATGTTAGGGATAAGGATAGGTTAAAAAGAGCTATTGAGGAG
GTAGATGTTGTATTCCATGCAGCTGCATTAAAGCACGTTCCCCTCTGCGAATACAACCCATTTGAAGCTGTAAAAACTAA
CGTTATTGGAACTCAAAATTTGATTGAAGTAGCAATGGATGAAGAAGTTGAAAAATTTATAACAATAAGCACAGACAAGG
CAGTAAATCCAGTAAATGTTATGGGCGCTACCAAATTATTGGCTGAAAGATTAACAATTTCAGCAAATTTATATAAAGGA
AAGAGAAAAACGGCTTTTTCTGTTGTTAGATTTGGAAATGTTCTAAATTCAAGAGGTTCCATACTGCCATTACTAAAAGA
ACAAATAAAAAAAGGAGGGCCTGTAACTTTAACCCATCCAGATATGACAAGATTTATAATGTCTATTAATGAAGCTGTTA
AATTAGTTTTAAAAGCTTGTTATTTGGCTAAAGGTGGGGAAATATTCATTTTAAAAATGCCTTCTGTTAGAATTAAAGAT
TTAATTGAGGTTGTTATTGAGGAACTCGCTCCAAAATATGGATATAAACCAGAAGATATTGAAATTAAAATTATTGGAAA
GAGGCCTGGTGAAAAACTATATGAAGAGTTAATTATCGAAGAAGAAATTTATAACTTAGAAGAGTTAGAAGATATGTTTG
TTGTTTATCCTTATGGAGTAGATGGAAATAAAAATAATAAGATAATTTATAATTCGAAGGATGCCAAATTTTTAAATAAA
GAGAAAATTAAAAAAATATTAAAAGAAATTAGTTATTTATAA

Upstream 100 bases:

>100_bases
TCGATCCATTAAACTTTTTGAATCATTAGGATTCAAAAAAACTAAAAAAGGTAGAGAAAACGAATGGATATACGAAGTGA
ATTTATAATAAGGTGAAAAA

Downstream 100 bases:

>100_bases
TTGTTTATTTAAAATTTCAAAAAATATTTCCTTTAAATTTTTTCTAAAATATTTTTTAAATTCTTTATCATCCATCATAA
ATGTTTCTTTTGCATTGGAT

Product: capsular polysaccharide biosynthesis protein D

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFQDISNFYKDKTILVTGGTGSIGKEIVKTLLKFNPKTIRVLDINETALFELEHELNSEKIRCFIGDVRDKDRLKRAIEE
VDVVFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIEVAMDEEVEKFITISTDKAVNPVNVMGATKLLAERLTISANLYKG
KRKTAFSVVRFGNVLNSRGSILPLLKEQIKKGGPVTLTHPDMTRFIMSINEAVKLVLKACYLAKGGEIFILKMPSVRIKD
LIEVVIEELAPKYGYKPEDIEIKIIGKRPGEKLYEELIIEEEIYNLEELEDMFVVYPYGVDGNKNNKIIYNSKDAKFLNK
EKIKKILKEISYL

Sequences:

>Translated_333_residues
MFQDISNFYKDKTILVTGGTGSIGKEIVKTLLKFNPKTIRVLDINETALFELEHELNSEKIRCFIGDVRDKDRLKRAIEE
VDVVFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIEVAMDEEVEKFITISTDKAVNPVNVMGATKLLAERLTISANLYKG
KRKTAFSVVRFGNVLNSRGSILPLLKEQIKKGGPVTLTHPDMTRFIMSINEAVKLVLKACYLAKGGEIFILKMPSVRIKD
LIEVVIEELAPKYGYKPEDIEIKIIGKRPGEKLYEELIIEEEIYNLEELEDMFVVYPYGVDGNKNNKIIYNSKDAKFLNK
EKIKKILKEISYL
>Mature_333_residues
MFQDISNFYKDKTILVTGGTGSIGKEIVKTLLKFNPKTIRVLDINETALFELEHELNSEKIRCFIGDVRDKDRLKRAIEE
VDVVFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIEVAMDEEVEKFITISTDKAVNPVNVMGATKLLAERLTISANLYKG
KRKTAFSVVRFGNVLNSRGSILPLLKEQIKKGGPVTLTHPDMTRFIMSINEAVKLVLKACYLAKGGEIFILKMPSVRIKD
LIEVVIEELAPKYGYKPEDIEIKIIGKRPGEKLYEELIIEEEIYNLEELEDMFVVYPYGVDGNKNNKIIYNSKDAKFLNK
EKIKKILKEISYL

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family

Homologues:

Organism=Homo sapiens, GI7657641, Length=265, Percent_Identity=26.0377358490566, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1061_METJA (Q58461)

Other databases:

- EMBL:   L77117
- PIR:   D64432
- RefSeq:   NP_248055.1
- ProteinModelPortal:   Q58461
- SMR:   Q58461
- GeneID:   1451958
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1061
- NMPDR:   fig|243232.1.peg.1092
- TIGR:   MJ1061
- HOGENOM:   HBG429349
- OMA:   IHAAAMK
- ProtClustDB:   CLSK796711
- BioCyc:   MJAN243232:MJ_1061-MONOMER
- GO:   GO:0005488
- InterPro:   IPR016040
- InterPro:   IPR003869
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF23

Pfam domain/function: PF02719 Polysacc_synt_2

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37847; Mature: 37847

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQDISNFYKDKTILVTGGTGSIGKEIVKTLLKFNPKTIRVLDINETALFELEHELNSEK
CCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHEEHHHHHCCCCE
IRCFIGDVRDKDRLKRAIEEVDVVFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIEVAMD
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHH
EEVEKFITISTDKAVNPVNVMGATKLLAERLTISANLYKGKRKTAFSVVRFGNVLNSRGS
HHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ILPLLKEQIKKGGPVTLTHPDMTRFIMSINEAVKLVLKACYLAKGGEIFILKMPSVRIKD
CHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LIEVVIEELAPKYGYKPEDIEIKIIGKRPGEKLYEELIIEEEIYNLEELEDMFVVYPYGV
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCCEEEEEEECC
DGNKNNKIIYNSKDAKFLNKEKIKKILKEISYL
CCCCCCEEEECCCCCHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFQDISNFYKDKTILVTGGTGSIGKEIVKTLLKFNPKTIRVLDINETALFELEHELNSEK
CCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHEEHHHHHCCCCE
IRCFIGDVRDKDRLKRAIEEVDVVFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIEVAMD
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHH
EEVEKFITISTDKAVNPVNVMGATKLLAERLTISANLYKGKRKTAFSVVRFGNVLNSRGS
HHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ILPLLKEQIKKGGPVTLTHPDMTRFIMSINEAVKLVLKACYLAKGGEIFILKMPSVRIKD
CHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LIEVVIEELAPKYGYKPEDIEIKIIGKRPGEKLYEELIIEEEIYNLEELEDMFVVYPYGV
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCCEEEEEEECC
DGNKNNKIIYNSKDAKFLNKEKIKKILKEISYL
CCCCCCEEEECCCCCHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087