Click here to switch to the map view.

The map label for this gene is hacA

Identifier: 15669192

GI number: 15669192

Start: 931995

End: 933257

Strand: Direct

Name: hacA

Synonym: MJ1003

Alternate gene names: 15669192

Gene position: 931995-933257 (Clockwise)

Preceding gene: 15669191

Following gene: 15669193

Centisome position: 55.98

GC content: 36.82

Gene sequence:

>1263_bases
TTGACATTGGTAGAGAAGATACTATCAAAAAAAGTTGGTTATGAAGTTTGTGCAGGAGATAGCATAGAGGTTGAAGTTGA
TTTGGCAATGACACACGATGGAACAACACCTTTAGCATACAAAGCTTTAAAGGAAATGAGTGATAGTGTTTGGAATCCAG
ATAAAATAGTCGTTGCCTTTGACCACAATGTTCCACCAAACACAGTTAAAGCTGCTGAAATGCAAAAATTAGCTTTGGAG
TTTGTTAAAAGATTTGGCATTAAAAATTTCCATAAAGGTGGAGAAGGCATCTGTCATCAAATCTTAGCTGAAAATTATGT
TTTGCCAAACATGTTTGTAGCTGGTGGAGACAGCCATACATGCACACATGGAGCTTTTGGAGCTTTTGCTACTGGCTTTG
GAGCTACTGATATGGCTTACATCTATGCAACAGGAGAAACATGGATTAAAGTGCCAAAAACAATTAGGGTAGATATAGTT
GGAAAAAATGAAAATGTTTCTGCCAAAGATATTGTTTTAAGGGTTTGTAAGGAAATTGGGAGAAGAGGAGCAACATACAT
GGCTATTGAGTATGGTGGAGAGGTTGTTAAAAACATGGACATGGATGGAAGGCTAACTTTATGCAACATGGCAATAGAGA
TGGGAGGAAAAACAGGAGTGATAGAGGCTGATGAAATTACTTATGATTATTTAAAGAAAGAGAGAGGACTTTCTGATGAG
GATATAGCTAAATTAAAAAAAGAGAGAATAACAGTAAATAGAGATGAAGCAAACTACTATAAGGAGATAGAAATTGACAT
AACAGATATGGAAGAACAAGTTGCTGTTCCACACCACCCAGATAACGTAAAGCCAATTAGTGATGTTGAAGGGACTGAGA
TAAATCAAGTTTTTATTGGGAGTTGCACAAATGGAAGGTTGAGTGATTTAAGAGAAGCAGCTAAATATTTAAAAGGTAGG
GAGGTTCATAAAGATGTTAAGCTAATTGTTATCCCGGCATCAAAAAAGGTATTTTTGCAAGCGTTAAAAGAGGGTATTAT
AGATATCTTTGTTAAAGCTGGGGCGATGATTTGCACTCCGGGATGCGGACCTTGCTTAGGAGCTCATCAAGGGGTTTTGG
CTGAGGGAGAAATTTGTTTATCAACAACAAACAGAAACTTTAAAGGAAGGATGGGGCATATAAATAGCTATATTTACTTG
GCATCTCCAAAGATTGCCGCAATAAGTGCAGTTAAGGGATATATAACCAACAAATTGGATTAA

Upstream 100 bases:

>100_bases
TTTGGTTATATTTTTTGCAAAAAAGTTATTAGGTTATTTGATAGAAAATTTGAACACCTTTCTTTGGGATGTTTTGTAAA
TAAAGTATTTAGGTGATAAA

Downstream 100 bases:

>100_bases
TGGAATAGGTATTAAAATTCCTAAAGAGATAGAAATAAAAACATTAAGCAAGTATAATATTATTTTATCATAAATATTCT
TTGTGATACTATGAAAGTTA

Product: 3-isopropylmalate dehydratase LeuC

Products: NA

Alternate protein names: HACN; (R)-homocitrate dehydratase; Homoaconitate hydratase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAFDHNVPPNTVKAAEMQKLALE
FVKRFGIKNFHKGGEGICHQILAENYVLPNMFVAGGDSHTCTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIV
GKNENVSAKDIVLRVCKEIGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLSDE
DIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVFIGSCTNGRLSDLREAAKYLKGR
EVHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMICTPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYL
ASPKIAAISAVKGYITNKLD

Sequences:

>Translated_420_residues
MTLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAFDHNVPPNTVKAAEMQKLALE
FVKRFGIKNFHKGGEGICHQILAENYVLPNMFVAGGDSHTCTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIV
GKNENVSAKDIVLRVCKEIGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLSDE
DIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVFIGSCTNGRLSDLREAAKYLKGR
EVHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMICTPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYL
ASPKIAAISAVKGYITNKLD
>Mature_419_residues
TLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAFDHNVPPNTVKAAEMQKLALEF
VKRFGIKNFHKGGEGICHQILAENYVLPNMFVAGGDSHTCTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIVG
KNENVSAKDIVLRVCKEIGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLSDED
IAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVFIGSCTNGRLSDLREAAKYLKGRE
VHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMICTPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLA
SPKIAAISAVKGYITNKLD

Specific function: Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxyla

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=29.8913043478261, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI8659555, Length=425, Percent_Identity=24.7058823529412, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=34.4827586206897, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI2367097, Length=427, Percent_Identity=25.5269320843091, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081781, Length=444, Percent_Identity=24.3243243243243, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787531, Length=430, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32564738, Length=367, Percent_Identity=31.0626702997275, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI25149337, Length=367, Percent_Identity=31.0626702997275, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI25149342, Length=286, Percent_Identity=30.4195804195804, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321429, Length=456, Percent_Identity=33.1140350877193, Blast_Score=212, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6320440, Length=424, Percent_Identity=31.6037735849057, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6322261, Length=384, Percent_Identity=30.46875, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=29.6703296703297, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI161076999, Length=373, Percent_Identity=29.4906166219839, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI281365315, Length=373, Percent_Identity=29.4906166219839, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17864292, Length=373, Percent_Identity=29.4906166219839, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI28571643, Length=368, Percent_Identity=30.1630434782609, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HACA_METJA (Q58409)

Other databases:

- EMBL:   L77117
- PIR:   B64425
- RefSeq:   NP_247997.1
- ProteinModelPortal:   Q58409
- SMR:   Q58409
- GeneID:   1451900
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1003
- NMPDR:   fig|243232.1.peg.1034
- TIGR:   MJ1003
- HOGENOM:   HBG330745
- OMA:   PENTKTI
- ProtClustDB:   CLSK876422
- BioCyc:   MetaCyc:MONOMER-2003
- BioCyc:   MJAN243232:MJ_1003-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.36

Molecular weight: Translated: 46064; Mature: 45932

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAF
CCHHHHHHHHHCCCEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
DHNVPPNTVKAAEMQKLALEFVKRFGIKNFHKGGEGICHQILAENYVLPNMFVAGGDSHT
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCC
CTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIVGKNENVSAKDIVLRVCKEIG
CCCCCCHHHHCCCCCCCEEEEEECCCEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHH
RRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLSDE
CCCCEEEEEECCCHHHHCCCCCCCEEEEEEHHHCCCCCCEEEHHHHHHHHHHHHCCCCHH
DIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVFIG
HHHHHHHHHEEEECCCCCCEEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCEECEEEEE
SCTNGRLSDLREAAKYLKGREVHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMICTP
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEECC
GCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLASPKIAAISAVKGYITNKLD
CCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAF
CHHHHHHHHHCCCEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
DHNVPPNTVKAAEMQKLALEFVKRFGIKNFHKGGEGICHQILAENYVLPNMFVAGGDSHT
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCC
CTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIVGKNENVSAKDIVLRVCKEIG
CCCCCCHHHHCCCCCCCEEEEEECCCEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHH
RRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLSDE
CCCCEEEEEECCCHHHHCCCCCCCEEEEEEHHHCCCCCCEEEHHHHHHHHHHHHCCCCHH
DIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVFIG
HHHHHHHHHEEEECCCCCCEEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCEECEEEEE
SCTNGRLSDLREAAKYLKGREVHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMICTP
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEECC
GCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLASPKIAAISAVKGYITNKLD
CCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087