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The map label for this gene is mjaIM

Identifier: 15669175

GI number: 15669175

Start: 916461

End: 917372

Strand: Reverse

Name: mjaIM

Synonym: MJ0985

Alternate gene names: 15669175

Gene position: 917372-916461 (Counterclockwise)

Preceding gene: 15669176

Following gene: 15669173

Centisome position: 55.1

GC content: 32.02

Gene sequence:

>912_bases
ATGATGAGTATTGATATAACAACTAATCACAAAATAATCTTTGGAGATGCAAGAAAAATGGATGAAATTGAGGATGAAAG
TGTGCATTTAGTTGTTACATCACCTCCATATCCAATGATAGAAATGTGGGATGAATTATTCAAAATGTTAAATCTAGAAA
TAAATAAGCGTTGGATGGAGATGGAAAATGAAGAAGATGAAGAGAAAAAAGAAAAATTAATCATGCAAATATATAATTTA
ATGCATCAAACATTATATCCAGTTTGGGAAGAAGTCTATAGAGTTTTAGTTCCAGGAGGAATTGCATGCATCAACATAGG
AGACGCTACAAGAAAAATAAACGGAGTTTTTAGACTATTTCCAAATCATTCTAAGATTATAGAAAACTTTGAAAAGATTG
GATTCGTTACTCTCCCATATATACTATGGAAGAAACCCTCAAACAAGCCAAATGCATTCTTAGGTTCTGGATTTCTTCCT
CCAAATGCTTATGTAACCTTAGATGTTGAGTATATATTAATATTTAGGAAAGGAAAACCAAGAAAGTTTAAACCAAAAGA
CCCGTTAAGATATGCAAGTGCATACACTAAGGAGGAGAGAGACAGATGGTTCTCTCAAATTTGGGAGATTGTTGGAGATA
AGCAAACACATCCAAAAATAGAGAGAAGAACGGCATCATTTCCAGAAGAGATTCCAAGAAGATTAATAAGGATGTTTTCT
ATAATTGGAGACACCGTCTTAGACCCTTTCTTAGGGACTGGAACAACAGTAAAAGCGGCTATTGAATTAAAAAGAAACTC
TATTGGATATGAAATTGATAAATCCCTAAAGCCAATAATTGAAGAAAAAATTGGAATTAAGCAAAAAAGAATAGGAATGG
ATTTTAATGTAGAATTTATTAATCGTGGTTAA

Upstream 100 bases:

>100_bases
TAGGACAAACTGATGCTGAAATTGTGAGAACCATTGTTTTAACATGGCTGTCTGAAAAATCAATTATATCAACCACCATA
AAGAAAGAAATAGGGGATAA

Downstream 100 bases:

>100_bases
TGATAATTACTTCGTATTTTCAACTAAATCCAATAAATCGGAGTAATCAACAATAATTTTATTTTTCTTCTTTTCATAAA
TAATTAAATGCCCTTTAAGT

Product: type II R/M system modification methyltransferase

Products: NA

Alternate protein names: M.MjaI; N-4 cytosine-specific methyltransferase MjaI

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MMSIDITTNHKIIFGDARKMDEIEDESVHLVVTSPPYPMIEMWDELFKMLNLEINKRWMEMENEEDEEKKEKLIMQIYNL
MHQTLYPVWEEVYRVLVPGGIACINIGDATRKINGVFRLFPNHSKIIENFEKIGFVTLPYILWKKPSNKPNAFLGSGFLP
PNAYVTLDVEYILIFRKGKPRKFKPKDPLRYASAYTKEERDRWFSQIWEIVGDKQTHPKIERRTASFPEEIPRRLIRMFS
IIGDTVLDPFLGTGTTVKAAIELKRNSIGYEIDKSLKPIIEEKIGIKQKRIGMDFNVEFINRG

Sequences:

>Translated_303_residues
MMSIDITTNHKIIFGDARKMDEIEDESVHLVVTSPPYPMIEMWDELFKMLNLEINKRWMEMENEEDEEKKEKLIMQIYNL
MHQTLYPVWEEVYRVLVPGGIACINIGDATRKINGVFRLFPNHSKIIENFEKIGFVTLPYILWKKPSNKPNAFLGSGFLP
PNAYVTLDVEYILIFRKGKPRKFKPKDPLRYASAYTKEERDRWFSQIWEIVGDKQTHPKIERRTASFPEEIPRRLIRMFS
IIGDTVLDPFLGTGTTVKAAIELKRNSIGYEIDKSLKPIIEEKIGIKQKRIGMDFNVEFINRG
>Mature_303_residues
MMSIDITTNHKIIFGDARKMDEIEDESVHLVVTSPPYPMIEMWDELFKMLNLEINKRWMEMENEEDEEKKEKLIMQIYNL
MHQTLYPVWEEVYRVLVPGGIACINIGDATRKINGVFRLFPNHSKIIENFEKIGFVTLPYILWKKPSNKPNAFLGSGFLP
PNAYVTLDVEYILIFRKGKPRKFKPKDPLRYASAYTKEERDRWFSQIWEIVGDKQTHPKIERRTASFPEEIPRRLIRMFS
IIGDTVLDPFLGTGTTVKAAIELKRNSIGYEIDKSLKPIIEEKIGIKQKRIGMDFNVEFINRG

Specific function: This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease (Potential)

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTM1_METJA (Q58392)

Other databases:

- EMBL:   L77117
- PIR:   A64423
- RefSeq:   NP_247980.1
- ProteinModelPortal:   Q58392
- REBASE:   3889
- GeneID:   1451883
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0985
- NMPDR:   fig|243232.1.peg.1017
- TIGR:   MJ0985
- HOGENOM:   HBG405684
- OMA:   MIEMWDE
- ProtClustDB:   CLSK782117
- BioCyc:   MJAN243232:MJ_0985-MONOMER
- BRENDA:   2.1.1.113
- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091
- PRINTS:   PR00508

Pfam domain/function: PF01555 N6_N4_Mtase

EC number: =2.1.1.113

Molecular weight: Translated: 35519; Mature: 35519

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSIDITTNHKIIFGDARKMDEIEDESVHLVVTSPPYPMIEMWDELFKMLNLEINKRWME
CEEEEEECCCEEEECCCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHH
MENEEDEEKKEKLIMQIYNLMHQTLYPVWEEVYRVLVPGGIACINIGDATRKINGVFRLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEEEC
PNHSKIIENFEKIGFVTLPYILWKKPSNKPNAFLGSGFLPPNAYVTLDVEYILIFRKGKP
CCHHHHHHHHHHCCHHEECHHEEECCCCCCCCEECCCCCCCCCEEEEEEEEEEEEECCCC
RKFKPKDPLRYASAYTKEERDRWFSQIWEIVGDKQTHPKIERRTASFPEEIPRRLIRMFS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
IIGDTVLDPFLGTGTTVKAAIELKRNSIGYEIDKSLKPIIEEKIGIKQKRIGMDFNVEFI
HHHHHHHHHHCCCCCEEEEHEEEHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEE
NRG
CCC
>Mature Secondary Structure
MMSIDITTNHKIIFGDARKMDEIEDESVHLVVTSPPYPMIEMWDELFKMLNLEINKRWME
CEEEEEECCCEEEECCCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHH
MENEEDEEKKEKLIMQIYNLMHQTLYPVWEEVYRVLVPGGIACINIGDATRKINGVFRLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEEEC
PNHSKIIENFEKIGFVTLPYILWKKPSNKPNAFLGSGFLPPNAYVTLDVEYILIFRKGKP
CCHHHHHHHHHHCCHHEECHHEEECCCCCCCCEECCCCCCCCCEEEEEEEEEEEEECCCC
RKFKPKDPLRYASAYTKEERDRWFSQIWEIVGDKQTHPKIERRTASFPEEIPRRLIRMFS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
IIGDTVLDPFLGTGTTVKAAIELKRNSIGYEIDKSLKPIIEEKIGIKQKRIGMDFNVEFI
HHHHHHHHHHCCCCCEEEEHEEEHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEE
NRG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087