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The map label for this gene is purD

Identifier: 15669127

GI number: 15669127

Start: 866108

End: 867442

Strand: Direct

Name: purD

Synonym: MJ0937

Alternate gene names: 15669127

Gene position: 866108-867442 (Clockwise)

Preceding gene: 15669126

Following gene: 15669129

Centisome position: 52.02

GC content: 35.06

Gene sequence:

>1335_bases
ATGAAAATTTTATTGATTGGAGGAGGAGCAAGGGAGAGTGCAATAGCTCACGCTCTAAAAAAGAATGAAGAAGTAAAGCT
CTACACATTAATGAAAAACAAAAACCCAGGAATTGCAAGATTATCAGAAGAGATAAAGTTAGCTAAAGAGACTGATTTAG
ATGCGGTTAAAGAGTTTGCTGAAAAAGTTAAACCAGATTTGGCTGTTATAGGTCCAGAAGCTCCTTTAGGGGAAGGTGTT
GTTGATTTGTTAGAGGAAATGGGAATTTCAGCAGTTGGTCCTAAAAAGTTAGCAGCACAAATAGAAACAAACAAAGAATT
CATGAGAAATTTATTCAAAAAATACAATATAAAAGGTTCTTTAATGTATAAAGCTTTTGAAGAGTATGGGGAAGAGTTAG
AAAGCTTTATTGATGAATTAACTGAGAAAGGTATTAAAGCAGTCGTTAAACCCGTTGGATTGACAGGAGGTAAAGGAGTT
AAGGTAGTTGGAGAACAGCTAAAAGATAATGAAGAGGCAAAGAAATATGCTAAAGAGATTTTTGAAACTGGTTTAGGGGG
AGGAAAGGTCTTAATTGAAGAAAAATTGGAAGGAGTTGAATTCACCTTACACGGATTTGTTGATGGAGATACTATTAAAT
TTACACCATTTGTCCAAGACCACCCACATGCATTAGAGGGGGATGAAGGAAGTATAACAGGAGGTATGGGTTCTTACTCA
TGCCCAGACCATAAACTACCATTTATGACAGAAGAGGATGTTAAGTTAGCAAAAGAGATTATGGAAGAGACTGTTAAAGC
ATTAAAGGAGGAGGTTGGAGGTTACAAAGGAATTTTATATGGACAGTTTATGCTAACAAAAGAGGGGCCGAAAATTATTG
AATACAATGCAAGATTTGGAGACCCTGAAGCAATGAACTTATTAGCTATATTAAAGAATGATTTCTTAGAGGTTTGTGAG
GCAATAGTAAATAAAAAACTTAAAGATATTGACGTTGAGTTTGAAAACAAAGCTACTGTTTGTAAGTATGTTGTTCCAAA
GGGATATCCTGATAACCCAGTTAGGGGAGAGCCAATAACTGTTGATGAAGAAGCAATTAAGAAAACTGGAGCTATATTGC
ATTATGCTTCAGTTAATGAGGATAATGGGTCTTTATATATGACTGGTTCAAGGGCTGTTGCTGTGGTTGGAGTTGCTGAT
ACAATTGAAGAAGCTGAGAGAATTGCTGAGGAGGCAACTAAATATATTAAAGGAGAAGTATATCACAGAAGTGATATTGG
TAAAAAAGAGTTAATTAAAAAGAGAATAGAGAAGATGAACGAATTAAGAAGATAA

Upstream 100 bases:

>100_bases
TGAAAAGAAGGAGTATAGGGAGATAGTGTTAGAGTAAAAGATTTAAACCACTTTTTTACTTTTTTATTATTACATTAATC
CAAAAGCAAAAGGTGAGATT

Downstream 100 bases:

>100_bases
AATTTAATTAAATTTTAAAACCTTTTTTATGCTCTAATAATCTCTTTATACATTAGGTAGCATATCAAAATCCCAGAAAT
TACTGCAGAGCTTAGAATCA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKILLIGGGARESAIAHALKKNEEVKLYTLMKNKNPGIARLSEEIKLAKETDLDAVKEFAEKVKPDLAVIGPEAPLGEGV
VDLLEEMGISAVGPKKLAAQIETNKEFMRNLFKKYNIKGSLMYKAFEEYGEELESFIDELTEKGIKAVVKPVGLTGGKGV
KVVGEQLKDNEEAKKYAKEIFETGLGGGKVLIEEKLEGVEFTLHGFVDGDTIKFTPFVQDHPHALEGDEGSITGGMGSYS
CPDHKLPFMTEEDVKLAKEIMEETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFGDPEAMNLLAILKNDFLEVCE
AIVNKKLKDIDVEFENKATVCKYVVPKGYPDNPVRGEPITVDEEAIKKTGAILHYASVNEDNGSLYMTGSRAVAVVGVAD
TIEEAERIAEEATKYIKGEVYHRSDIGKKELIKKRIEKMNELRR

Sequences:

>Translated_444_residues
MKILLIGGGARESAIAHALKKNEEVKLYTLMKNKNPGIARLSEEIKLAKETDLDAVKEFAEKVKPDLAVIGPEAPLGEGV
VDLLEEMGISAVGPKKLAAQIETNKEFMRNLFKKYNIKGSLMYKAFEEYGEELESFIDELTEKGIKAVVKPVGLTGGKGV
KVVGEQLKDNEEAKKYAKEIFETGLGGGKVLIEEKLEGVEFTLHGFVDGDTIKFTPFVQDHPHALEGDEGSITGGMGSYS
CPDHKLPFMTEEDVKLAKEIMEETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFGDPEAMNLLAILKNDFLEVCE
AIVNKKLKDIDVEFENKATVCKYVVPKGYPDNPVRGEPITVDEEAIKKTGAILHYASVNEDNGSLYMTGSRAVAVVGVAD
TIEEAERIAEEATKYIKGEVYHRSDIGKKELIKKRIEKMNELRR
>Mature_444_residues
MKILLIGGGARESAIAHALKKNEEVKLYTLMKNKNPGIARLSEEIKLAKETDLDAVKEFAEKVKPDLAVIGPEAPLGEGV
VDLLEEMGISAVGPKKLAAQIETNKEFMRNLFKKYNIKGSLMYKAFEEYGEELESFIDELTEKGIKAVVKPVGLTGGKGV
KVVGEQLKDNEEAKKYAKEIFETGLGGGKVLIEEKLEGVEFTLHGFVDGDTIKFTPFVQDHPHALEGDEGSITGGMGSYS
CPDHKLPFMTEEDVKLAKEIMEETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFGDPEAMNLLAILKNDFLEVCE
AIVNKKLKDIDVEFENKATVCKYVVPKGYPDNPVRGEPITVDEEAIKKTGAILHYASVNEDNGSLYMTGSRAVAVVGVAD
TIEEAERIAEEATKYIKGEVYHRSDIGKKELIKKRIEKMNELRR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=438, Percent_Identity=35.6164383561644, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI209869995, Length=438, Percent_Identity=35.6164383561644, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI209869993, Length=438, Percent_Identity=35.6164383561644, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI28416899, Length=438, Percent_Identity=35.6164383561644, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1790438, Length=438, Percent_Identity=31.7351598173516, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17567511, Length=436, Percent_Identity=33.7155963302752, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321203, Length=452, Percent_Identity=28.5398230088496, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI62471619, Length=447, Percent_Identity=31.3199105145414, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24582400, Length=446, Percent_Identity=31.6143497757848, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_METJA (Q58347)

Other databases:

- EMBL:   L77117
- PIR:   A64417
- RefSeq:   NP_247932.1
- ProteinModelPortal:   Q58347
- GeneID:   1451833
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0937
- NMPDR:   fig|243232.1.peg.969
- TIGR:   MJ0937
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   MJAN243232:MJ_0937-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 49098; Mature: 49098

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLIGGGARESAIAHALKKNEEVKLYTLMKNKNPGIARLSEEIKLAKETDLDAVKEFA
CEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHH
EKVKPDLAVIGPEAPLGEGVVDLLEEMGISAVGPKKLAAQIETNKEFMRNLFKKYNIKGS
HHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCHH
LMYKAFEEYGEELESFIDELTEKGIKAVVKPVGLTGGKGVKVVGEQLKDNEEAKKYAKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
FETGLGGGKVLIEEKLEGVEFTLHGFVDGDTIKFTPFVQDHPHALEGDEGSITGGMGSYS
HHCCCCCCEEEEEHHCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEECCCCCCC
CPDHKLPFMTEEDVKLAKEIMEETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEECCCCC
DPEAMNLLAILKNDFLEVCEAIVNKKLKDIDVEFENKATVCKYVVPKGYPDNPVRGEPIT
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEECCCCCEEEEECCCCCCCCCCCCCCEE
VDEEAIKKTGAILHYASVNEDNGSLYMTGSRAVAVVGVADTIEEAERIAEEATKYIKGEV
CCHHHHHHHCCEEEEEECCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHCCCE
YHRSDIGKKELIKKRIEKMNELRR
EECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILLIGGGARESAIAHALKKNEEVKLYTLMKNKNPGIARLSEEIKLAKETDLDAVKEFA
CEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHH
EKVKPDLAVIGPEAPLGEGVVDLLEEMGISAVGPKKLAAQIETNKEFMRNLFKKYNIKGS
HHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCHH
LMYKAFEEYGEELESFIDELTEKGIKAVVKPVGLTGGKGVKVVGEQLKDNEEAKKYAKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
FETGLGGGKVLIEEKLEGVEFTLHGFVDGDTIKFTPFVQDHPHALEGDEGSITGGMGSYS
HHCCCCCCEEEEEHHCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEECCCCCCC
CPDHKLPFMTEEDVKLAKEIMEETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEECCCCC
DPEAMNLLAILKNDFLEVCEAIVNKKLKDIDVEFENKATVCKYVVPKGYPDNPVRGEPIT
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEECCCCCEEEEECCCCCCCCCCCCCCEE
VDEEAIKKTGAILHYASVNEDNGSLYMTGSRAVAVVGVADTIEEAERIAEEATKYIKGEV
CCHHHHHHHCCEEEEEECCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHCCCE
YHRSDIGKKELIKKRIEKMNELRR
EECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087