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The map label for this gene is rfcL

Identifier: 15669074

GI number: 15669074

Start: 809844

End: 811394

Strand: Direct

Name: rfcL

Synonym: MJ0884

Alternate gene names: 15669074

Gene position: 809844-811394 (Clockwise)

Preceding gene: 15669073

Following gene: 15669079

Centisome position: 48.64

GC content: 33.46

Gene sequence:

>1551_bases
ATGTTAAGTTGGGTAGAGAAGTATAGGCCAAAATCATTGAAAGATGTTGCTGGGCATGAAAAGGTTAAAGAGAAACTAAA
AACATGGATTGAAAGTTATTTAAAAGGGGAAACTCCAAAACCGATTTTGCTTGTAGGCCCTCCGGGATGTGGAAAAACAA
CATTGGCTTATGCATTAGCAAACGATTACGGATTTGAGGTTATTGAACTCAATGCAAGTGATAAAAGAAATTCTTCTGCA
ATAAAAAAGGTTGTAGGGCATGCTGCTACTTCCTCATCCATCTTTGGAAAAAAATTTTTAATTGTATTAGATGAGGTTGA
TGGAATCTCTGGAAAGGAAGATGCTGGAGGGGTCTCTGAGCTTATAAAGGTTATAAAAAAGGCAAAGAACCCAATAATTT
TAACTGCAAACGATGCTTACGCTCCATCAATAAGGAGTCTTCTACCTTATGTTGAGGTAATTCAATTAAATCCAGTGCAT
ACAAACTCAGTCTATAAAGTTCTAAAAAAGATAGCTGAGAAAGAGGGGCTTGATGTGGATGATAAAACGCTAAAGATGAT
TGCTCAACATTCAGCTGGAGATTTGAGGAGTGCAATAAATGACTTAGAGGCTTTAGCTTTATCTGGAGATTTAAGTTATG
AAGCAGCTCAAAAATTGCCAGATAGAAAGAGAGAGGCAAATATCTTCGATGCTTTAAGAGTTATTTTAAAAACTACTCAC
TATGGAATAGCTACAACTGCCTTAATGAATGTTGATGAAACGCCAGACGTTGTAATTGAATGGATAGCTGAAAACGTTCC
AAAAGAGTATGAAAAGCCAGAAGAAGTTGCAAGGGCTTTTGAATATTTATCAAAGGCAGATCGATATTTAGGTAGAGTGA
TGAGAAGACAAAACTATAGTTTTTGGAAGTATGCTACAACGTTAATGACTGCTGGTGTTGCTCTCTCAAAGGATGAGAAG
TATAGGAAATGGACACCTTACAGTTATCCTAAGATTTTTAGATTATTAACAAAAACAAAGGCAGAGAGGGAGATATTAAA
TAAAATATTAAAGAAAATTGGTGAAAAAACCCATACATCCTCAAAGAGAGCAAGGTTTGATTTGCAGATGCTTAAACTCT
TAGCTAAAGAAAATCCTTCTGTAGCTGCTGATTTAGTTGATTACTTTGAGATAAAGGAGGATGAGCTAAAAGTTTTAGTT
GGAGATAAGTTAGCTTCTGAGATATTGAAGATATTAAAAGAAAAGAAAAAATTGGAAAGAAAGAAGAAAAAAGAGAAAGA
GAAGTTAGAGAAAGAAAAGAAGAAAGAAGAAAAAGCTAAGGAAAAACAGTCAAATCTGATTATACAACCTAAAGAAATTA
AAGAAGAGGTAAAAGCTGAAGTAGAGAAAAAAGAAGAAGTTAAAGAAAAGATAGTAGAAAAACCAAAAGCTGAAGAAGTA
AAAGAAAAATCAAAAACAGAAGAAAAAGAAACTAAAAAAGATAAAAAGAAGGGAAAGAAAAAGAAAGAAGATAAAGGAAA
GCAACTAACTTTAGATGCTTTCTTCAAATAA

Upstream 100 bases:

>100_bases
AATTATTGTTTTATTTGCAATACAAATAACAAATCAAACGTAGTTGAGTTAATAAAACTATTATTTGGATTAAAATAATT
AGCTATAAAAGGGATAATCT

Downstream 100 bases:

>100_bases
ATTTATTTAAAAAACTTATCTAATGTTAATTGAGCTCCTTCTTTCTTCAACTCATTTTTTGAAACTCCTACGGCTTCCAT
AATTCTCAAAACTGGAGGAA

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MLSWVEKYRPKSLKDVAGHEKVKEKLKTWIESYLKGETPKPILLVGPPGCGKTTLAYALANDYGFEVIELNASDKRNSSA
IKKVVGHAATSSSIFGKKFLIVLDEVDGISGKEDAGGVSELIKVIKKAKNPIILTANDAYAPSIRSLLPYVEVIQLNPVH
TNSVYKVLKKIAEKEGLDVDDKTLKMIAQHSAGDLRSAINDLEALALSGDLSYEAAQKLPDRKREANIFDALRVILKTTH
YGIATTALMNVDETPDVVIEWIAENVPKEYEKPEEVARAFEYLSKADRYLGRVMRRQNYSFWKYATTLMTAGVALSKDEK
YRKWTPYSYPKIFRLLTKTKAEREILNKILKKIGEKTHTSSKRARFDLQMLKLLAKENPSVAADLVDYFEIKEDELKVLV
GDKLASEILKILKEKKKLERKKKKEKEKLEKEKKKEEKAKEKQSNLIIQPKEIKEEVKAEVEKKEEVKEKIVEKPKAEEV
KEKSKTEEKETKKDKKKGKKKKEDKGKQLTLDAFFK

Sequences:

>Translated_516_residues
MLSWVEKYRPKSLKDVAGHEKVKEKLKTWIESYLKGETPKPILLVGPPGCGKTTLAYALANDYGFEVIELNASDKRNSSA
IKKVVGHAATSSSIFGKKFLIVLDEVDGISGKEDAGGVSELIKVIKKAKNPIILTANDAYAPSIRSLLPYVEVIQLNPVH
TNSVYKVLKKIAEKEGLDVDDKTLKMIAQHSAGDLRSAINDLEALALSGDLSYEAAQKLPDRKREANIFDALRVILKTTH
YGIATTALMNVDETPDVVIEWIAENVPKEYEKPEEVARAFEYLSKADRYLGRVMRRQNYSFWKYATTLMTAGVALSKDEK
YRKWTPYSYPKIFRLLTKTKAEREILNKILKKIGEKTHTSSKRARFDLQMLKLLAKENPSVAADLVDYFEIKEDELKVLV
GDKLASEILKILKEKKKLERKKKKEKEKLEKEKKKEEKAKEKQSNLIIQPKEIKEEVKAEVEKKEEVKEKIVEKPKAEEV
KEKSKTEEKETKKDKKKGKKKKEDKGKQLTLDAFFK
>Mature_516_residues
MLSWVEKYRPKSLKDVAGHEKVKEKLKTWIESYLKGETPKPILLVGPPGCGKTTLAYALANDYGFEVIELNASDKRNSSA
IKKVVGHAATSSSIFGKKFLIVLDEVDGISGKEDAGGVSELIKVIKKAKNPIILTANDAYAPSIRSLLPYVEVIQLNPVH
TNSVYKVLKKIAEKEGLDVDDKTLKMIAQHSAGDLRSAINDLEALALSGDLSYEAAQKLPDRKREANIFDALRVILKTTH
YGIATTALMNVDETPDVVIEWIAENVPKEYEKPEEVARAFEYLSKADRYLGRVMRRQNYSFWKYATTLMTAGVALSKDEK
YRKWTPYSYPKIFRLLTKTKAEREILNKILKKIGEKTHTSSKRARFDLQMLKLLAKENPSVAADLVDYFEIKEDELKVLV
GDKLASEILKILKEKKKLERKKKKEKEKLEKEKKKEEKAKEKQSNLIIQPKEIKEEVKAEVEKKEEVKEKIVEKPKAEEV
KEKSKTEEKETKKDKKKGKKKKEDKGKQLTLDAFFK

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=272, Percent_Identity=32.3529411764706, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI6677723, Length=241, Percent_Identity=30.7053941908714, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI194306571, Length=239, Percent_Identity=29.7071129707113, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194306567, Length=239, Percent_Identity=29.7071129707113, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4506491, Length=286, Percent_Identity=29.020979020979, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI31881687, Length=286, Percent_Identity=29.020979020979, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI31563534, Length=263, Percent_Identity=26.2357414448669, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563226, Length=241, Percent_Identity=33.195020746888, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17541368, Length=419, Percent_Identity=24.8210023866348, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17541988, Length=208, Percent_Identity=31.7307692307692, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554730, Length=224, Percent_Identity=29.9107142857143, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71986063, Length=228, Percent_Identity=31.140350877193, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324791, Length=225, Percent_Identity=32, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323724, Length=269, Percent_Identity=27.5092936802974, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324039, Length=264, Percent_Identity=28.7878787878788, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324478, Length=215, Percent_Identity=28.8372093023256, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322528, Length=238, Percent_Identity=30.2521008403361, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17737765, Length=267, Percent_Identity=28.4644194756554, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI62472391, Length=289, Percent_Identity=26.9896193771626, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28574716, Length=391, Percent_Identity=27.8772378516624, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19921076, Length=217, Percent_Identity=28.110599078341, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17647857, Length=228, Percent_Identity=27.1929824561404, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI18859927, Length=218, Percent_Identity=29.3577981651376, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_METJA (Q58294)

Other databases:

- EMBL:   L77117
- PIR:   D64410
- RefSeq:   NP_247879.1
- ProteinModelPortal:   Q58294
- SMR:   Q58294
- GeneID:   1451773
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0884
- NMPDR:   fig|243232.1.peg.916
- TIGR:   MJ0884
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- ProtClustDB:   PRK04195
- BioCyc:   MJAN243232:MJ_0884-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: 3.4.24.- [C]

Molecular weight: Translated: 58771; Mature: 58771

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWVEKYRPKSLKDVAGHEKVKEKLKTWIESYLKGETPKPILLVGPPGCGKTTLAYALA
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHH
NDYGFEVIELNASDKRNSSAIKKVVGHAATSSSIFGKKFLIVLDEVDGISGKEDAGGVSE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCEEEEEEECCCCCCCCCCCCCHHH
LIKVIKKAKNPIILTANDAYAPSIRSLLPYVEVIQLNPVHTNSVYKVLKKIAEKEGLDVD
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
DKTLKMIAQHSAGDLRSAINDLEALALSGDLSYEAAQKLPDRKREANIFDALRVILKTTH
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHC
YGIATTALMNVDETPDVVIEWIAENVPKEYEKPEEVARAFEYLSKADRYLGRVMRRQNYS
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
FWKYATTLMTAGVALSKDEKYRKWTPYSYPKIFRLLTKTKAEREILNKILKKIGEKTHTS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SKRARFDLQMLKLLAKENPSVAADLVDYFEIKEDELKVLVGDKLASEILKILKEKKKLER
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKKEKEKLEKEKKKEEKAKEKQSNLIIQPKEIKEEVKAEVEKKEEVKEKIVEKPKAEEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KEKSKTEEKETKKDKKKGKKKKEDKGKQLTLDAFFK
HHHHHHHHHHHHHHHHHCCCCHHHCCCEEEHHHHCC
>Mature Secondary Structure
MLSWVEKYRPKSLKDVAGHEKVKEKLKTWIESYLKGETPKPILLVGPPGCGKTTLAYALA
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHH
NDYGFEVIELNASDKRNSSAIKKVVGHAATSSSIFGKKFLIVLDEVDGISGKEDAGGVSE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCEEEEEEECCCCCCCCCCCCCHHH
LIKVIKKAKNPIILTANDAYAPSIRSLLPYVEVIQLNPVHTNSVYKVLKKIAEKEGLDVD
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
DKTLKMIAQHSAGDLRSAINDLEALALSGDLSYEAAQKLPDRKREANIFDALRVILKTTH
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHC
YGIATTALMNVDETPDVVIEWIAENVPKEYEKPEEVARAFEYLSKADRYLGRVMRRQNYS
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
FWKYATTLMTAGVALSKDEKYRKWTPYSYPKIFRLLTKTKAEREILNKILKKIGEKTHTS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SKRARFDLQMLKLLAKENPSVAADLVDYFEIKEDELKVLVGDKLASEILKILKEKKKLER
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKKEKEKLEKEKKKEEKAKEKQSNLIIQPKEIKEEVKAEVEKKEEVKEKIVEKPKAEEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KEKSKTEEKETKKDKKKGKKKKEDKGKQLTLDAFFK
HHHHHHHHHHHHHHHHHCCCCHHHCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087