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The map label for this gene is argF

Identifier: 15669071

GI number: 15669071

Start: 806512

End: 807429

Strand: Reverse

Name: argF

Synonym: MJ0881

Alternate gene names: 15669071

Gene position: 807429-806512 (Counterclockwise)

Preceding gene: 15669075

Following gene: 15669067

Centisome position: 48.5

GC content: 33.88

Gene sequence:

>918_bases
ATGCATCTCTTAGATTTGGATGTGTTGAGTAGAGAAGATGTACTAAAAATTATTGAATATGGAATATACTTCAAAAAAAA
TAGAAGAAAACATGAAAAAATCTTAGAAGGGAAGAGTGTAGCGATTTTATTTGAAAAACCCTCAACAAGAACAAGAATGA
GTTTTGATATTGCAGTTTATGAGTTGGGAGGGCATCCACTAATAATGAACCAGAATGAGATACATTTAGGAAAGAAAGAG
TCAATAAAAGATACTGCAAAGGTTATGGGCAGATATGTTGATACTATAGTGGCAAGGGTCTATAAGCATAGACATTTAGA
GGAGATGGCTAAATATTCCTCAGTTCCTGTTATAAATGCTTTAAGCGATTTAGCTCACCCATGCCAAATATTGGCTGATT
TGATGACTATAAAAGAGTATAAAGGCAAATTCAAAGGTTTAAAAATAGCTTATTTAGGAGATGGAAATAACGTCTGTAAT
TCTTTAATTTTAGGCTCTGCTTTAGTAGGAATGGATACTTATGTGGGAACACCAAAAGGTTATGAACCTAATGCTAAAGT
TGTCTTAAAAGCTAAGGAGATTATTAATAATTATGGAGAAGGTTCTTTAACATTAACCAACGACCCAATAGAGGCAGCTG
AAGATGCTGATGTATTATACACCGACGTATGGATTAGTATGGGTGATGATAAAGACAAAGAAGAGGTTTTAAAAATCTTT
CCACCATTCCAAATTAATAGCAAGCTCTTAGAGTATGCTAAAGATGATGTTATAGTTATGCACTGCCTCCCAGCAAATAG
AGGATATGAGATAACAGACGATGTTATTGACGGAGAGCATTCAGTTGTCTATGATGAGGCTGAGAATAGGTTACATGTTC
AGAAGGGAGTATTTAAGTTTATATTTGAGAGAAAGTAA

Upstream 100 bases:

>100_bases
TTTTGCAAATATCGTCTATCATTACGTAAGAAACTACAACAATATAAATAATGGCTCATGATAATATAAAATAGTTTTTA
ATAGTATAAAAGGTGATAAA

Downstream 100 bases:

>100_bases
TCTAAGAGGCACTGCCGAGCGTAGCGAGGCAGTGTATCCTGTTTTGATGAAACCGAAGCGTTAGCTTCGGGCTACAAAAA
CTTTTCGGGTTTTTGTTTAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MHLLDLDVLSREDVLKIIEYGIYFKKNRRKHEKILEGKSVAILFEKPSTRTRMSFDIAVYELGGHPLIMNQNEIHLGKKE
SIKDTAKVMGRYVDTIVARVYKHRHLEEMAKYSSVPVINALSDLAHPCQILADLMTIKEYKGKFKGLKIAYLGDGNNVCN
SLILGSALVGMDTYVGTPKGYEPNAKVVLKAKEIINNYGEGSLTLTNDPIEAAEDADVLYTDVWISMGDDKDKEEVLKIF
PPFQINSKLLEYAKDDVIVMHCLPANRGYEITDDVIDGEHSVVYDEAENRLHVQKGVFKFIFERK

Sequences:

>Translated_305_residues
MHLLDLDVLSREDVLKIIEYGIYFKKNRRKHEKILEGKSVAILFEKPSTRTRMSFDIAVYELGGHPLIMNQNEIHLGKKE
SIKDTAKVMGRYVDTIVARVYKHRHLEEMAKYSSVPVINALSDLAHPCQILADLMTIKEYKGKFKGLKIAYLGDGNNVCN
SLILGSALVGMDTYVGTPKGYEPNAKVVLKAKEIINNYGEGSLTLTNDPIEAAEDADVLYTDVWISMGDDKDKEEVLKIF
PPFQINSKLLEYAKDDVIVMHCLPANRGYEITDDVIDGEHSVVYDEAENRLHVQKGVFKFIFERK
>Mature_305_residues
MHLLDLDVLSREDVLKIIEYGIYFKKNRRKHEKILEGKSVAILFEKPSTRTRMSFDIAVYELGGHPLIMNQNEIHLGKKE
SIKDTAKVMGRYVDTIVARVYKHRHLEEMAKYSSVPVINALSDLAHPCQILADLMTIKEYKGKFKGLKIAYLGDGNNVCN
SLILGSALVGMDTYVGTPKGYEPNAKVVLKAKEIINNYGEGSLTLTNDPIEAAEDADVLYTDVWISMGDDKDKEEVLKIF
PPFQINSKLLEYAKDDVIVMHCLPANRGYEITDDVIDGEHSVVYDEAENRLHVQKGVFKFIFERK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=265, Percent_Identity=44.1509433962264, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=26.6881028938907, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=37.2670807453416, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1786469, Length=297, Percent_Identity=38.047138047138, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=28.4345047923323, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994908, Length=315, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=289, Percent_Identity=28.3737024221453, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=37.741935483871, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=298, Percent_Identity=29.5302013422819, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=26.797385620915, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_METJA (Q58291)

Other databases:

- EMBL:   L77117
- PIR:   A64410
- RefSeq:   NP_247876.1
- ProteinModelPortal:   Q58291
- SMR:   Q58291
- GeneID:   1451770
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0881
- NMPDR:   fig|243232.1.peg.913
- TIGR:   MJ0881
- HOGENOM:   HBG579429
- OMA:   FTVAMYQ
- ProtClustDB:   PRK00779
- BioCyc:   MJAN243232:MJ_0881-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34569; Mature: 34569

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 99-99 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLDLDVLSREDVLKIIEYGIYFKKNRRKHEKILEGKSVAILFEKPSTRTRMSFDIAVY
CCEEECHHHCHHHHHHHHHHHHEEECCCHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEE
ELGGHPLIMNQNEIHLGKKESIKDTAKVMGRYVDTIVARVYKHRHLEEMAKYSSVPVINA
EECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LSDLAHPCQILADLMTIKEYKGKFKGLKIAYLGDGNNVCNSLILGSALVGMDTYVGTPKG
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
YEPNAKVVLKAKEIINNYGEGSLTLTNDPIEAAEDADVLYTDVWISMGDDKDKEEVLKIF
CCCCCEEEEEEHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHC
PPFQINSKLLEYAKDDVIVMHCLPANRGYEITDDVIDGEHSVVYDEAENRLHVQKGVFKF
CCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCCEEEEECCCCEEHHHHHHHHH
IFERK
HHCCC
>Mature Secondary Structure
MHLLDLDVLSREDVLKIIEYGIYFKKNRRKHEKILEGKSVAILFEKPSTRTRMSFDIAVY
CCEEECHHHCHHHHHHHHHHHHEEECCCHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEE
ELGGHPLIMNQNEIHLGKKESIKDTAKVMGRYVDTIVARVYKHRHLEEMAKYSSVPVINA
EECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LSDLAHPCQILADLMTIKEYKGKFKGLKIAYLGDGNNVCNSLILGSALVGMDTYVGTPKG
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
YEPNAKVVLKAKEIINNYGEGSLTLTNDPIEAAEDADVLYTDVWISMGDDKDKEEVLKIF
CCCCCEEEEEEHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHC
PPFQINSKLLEYAKDDVIVMHCLPANRGYEITDDVIDGEHSVVYDEAENRLHVQKGVFKF
CCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCCEEEEECCCCEEHHHHHHHHH
IFERK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087