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The map label for this gene is mcrB

Identifier: 15669032

GI number: 15669032

Start: 768444

End: 769787

Strand: Direct

Name: mcrB

Synonym: MJ0842

Alternate gene names: 15669032

Gene position: 768444-769787 (Clockwise)

Preceding gene: 15669030

Following gene: 15669033

Centisome position: 46.15

GC content: 38.76

Gene sequence:

>1344_bases
GTGATTCCTATGGTAAAGTACAAAGACACAATAAACTTGTATGATGAAAAAGGTAAATTAGTAGAAGAAAACGTTCCTTT
AGAAGCAATCAGCCCATTGCACAACCCAACAATTCAGAAGTTAGTTAAAGATGTTAAGAGAACAGTGGCAGTTAACTTAG
CAGGTATTGAGAACGCTTTGAGAACTGGTCAAGTTGGTGGAAAAGGTTGTATGATTAAAGGTAGAGAGTTAGATTTACCA
ATTGTTGAAAATGCTGAAACAATTGCTGAATACGTTGAAAAAGTTGTTAGAGTTTCAGAAGATGACGACACATCAATTAA
ATTAATTAACGATGGAAAACAGATGGCTGTTCAAATTCCATCAAAGAGGTTAGATGTTGCAGCAGAATATTCAGTCTCTG
TTTTAGTTACAGCTCAAGCATTAAAAGAAGCTATTATTAGAACATTTGATGTTGATATCTTCGATGCTCCAATGGTTCAT
GCAGCTGTTTTAGGTGGCTACCCACACGAAGTTACAATGAAAGGTTCAAACATCGCTGCATTATTAGGTAGCCCATTATC
ATTAGAAGGGCCAGGTTATGCGTTAAGAAACATCATGGCAAACCACTTCGTTGCATGTACAAAGAAAAATGTCATGAACG
CAGTTGCATTCGCTGCAATTATGGAGCAAACAGCAATGTTTGAAATGGGAGACGCTGTTGGATTATTTGAAAGATTACAC
TTATTAGGTTTAGCATACCAAGGATTAAACGCAGACAACTTAGTAATTGACTTAGTTAAAGCAAACGGTAAGAACGGAAC
TGTTGGTACAGTAGTTGCTTCCGTTGTTGAAAGAGCTTTAGAGGATGGAGTCATTAAAGAAGACAAAACATTACCATCAG
GATTTAAGTTATACAAACCAGTTGATGTTGCTAAGTGGAACGCTTACGCTGCAGCTGGTTTAGTTGCAGCTGCTATAGTA
AACTGTGGGGCTGCAAGAGCAGCACAGAACATTGCATCAACAATCTTATACTACAACGACATCTTAGAATACGAAACAGG
ATTACCAGGAGTTGACTTCGGTAGAGCAGAAGGTACAGCAGTTGGATTCTCATTCTTCAGTCACTCAATTTACGGAGGAG
GAGGGCCAGGTATCTTCAACGGAAACCACATCGTTACAAGACACTCAAAAGGATTTGCTATCCCGCCAGTTGCAGCTGCA
ATGTGTTTAGACGCTGGAACACAGATGTTCTCACCAGAGAGAACATCAGCATTAGTTGGAACTGTCTTCAGTGCAATTGA
TGAATTCAGAGAGCCATTGAAGTATGTCATAAAAGGAGCATTAGAAGTTAAGGACAAAATCTAA

Upstream 100 bases:

>100_bases
AATTCCAAATCCATCCACTATATAAATGTTACGATTTTGTTCAAATGAAAAACTGGATATATATAAACTGCGATAACTAA
TATCGAACCAATATGTGGGA

Downstream 100 bases:

>100_bases
AACTTTAAGTGATTGTTTATGATTGAAGTTGAAATCTTCCCACATAGGTATTTGAAAGCCTCCACAACTGAAAAATTTCT
CAATAAAATTTATGACTTGA

Product: methyl coenzyme M reductase I subunit McrB

Products: NA

Alternate protein names: MCR I beta; Coenzyme-B sulfoethylthiotransferase beta

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIPMVKYKDTINLYDEKGKLVEENVPLEAISPLHNPTIQKLVKDVKRTVAVNLAGIENALRTGQVGGKGCMIKGRELDLP
IVENAETIAEYVEKVVRVSEDDDTSIKLINDGKQMAVQIPSKRLDVAAEYSVSVLVTAQALKEAIIRTFDVDIFDAPMVH
AAVLGGYPHEVTMKGSNIAALLGSPLSLEGPGYALRNIMANHFVACTKKNVMNAVAFAAIMEQTAMFEMGDAVGLFERLH
LLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVIKEDKTLPSGFKLYKPVDVAKWNAYAAAGLVAAAIV
NCGAARAAQNIASTILYYNDILEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPPVAAA
MCLDAGTQMFSPERTSALVGTVFSAIDEFREPLKYVIKGALEVKDKI

Sequences:

>Translated_447_residues
MIPMVKYKDTINLYDEKGKLVEENVPLEAISPLHNPTIQKLVKDVKRTVAVNLAGIENALRTGQVGGKGCMIKGRELDLP
IVENAETIAEYVEKVVRVSEDDDTSIKLINDGKQMAVQIPSKRLDVAAEYSVSVLVTAQALKEAIIRTFDVDIFDAPMVH
AAVLGGYPHEVTMKGSNIAALLGSPLSLEGPGYALRNIMANHFVACTKKNVMNAVAFAAIMEQTAMFEMGDAVGLFERLH
LLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVIKEDKTLPSGFKLYKPVDVAKWNAYAAAGLVAAAIV
NCGAARAAQNIASTILYYNDILEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPPVAAA
MCLDAGTQMFSPERTSALVGTVFSAIDEFREPLKYVIKGALEVKDKI
>Mature_447_residues
MIPMVKYKDTINLYDEKGKLVEENVPLEAISPLHNPTIQKLVKDVKRTVAVNLAGIENALRTGQVGGKGCMIKGRELDLP
IVENAETIAEYVEKVVRVSEDDDTSIKLINDGKQMAVQIPSKRLDVAAEYSVSVLVTAQALKEAIIRTFDVDIFDAPMVH
AAVLGGYPHEVTMKGSNIAALLGSPLSLEGPGYALRNIMANHFVACTKKNVMNAVAFAAIMEQTAMFEMGDAVGLFERLH
LLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVIKEDKTLPSGFKLYKPVDVAKWNAYAAAGLVAAAIV
NCGAARAAQNIASTILYYNDILEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPPVAAA
MCLDAGTQMFSPERTSALVGTVFSAIDEFREPLKYVIKGALEVKDKI

Specific function: Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide

COG id: COG4054

COG function: function code H; Methyl coenzyme M reductase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MCRB_METJA (Q58252)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247836.1
- ProteinModelPortal:   Q58252
- SMR:   Q58252
- GeneID:   1451730
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0842
- NMPDR:   fig|243232.1.peg.873
- TIGR:   MJ0842
- HOGENOM:   HBG538593
- OMA:   GFAIPPV
- ProtClustDB:   CLSK876059
- BioCyc:   MJAN243232:MJ_0842-MONOMER
- BRENDA:   2.8.4.1
- GO:   GO:0015948
- InterPro:   IPR022681
- InterPro:   IPR008924
- InterPro:   IPR015823
- InterPro:   IPR003179
- InterPro:   IPR022679
- InterPro:   IPR022680
- InterPro:   IPR009024
- Gene3D:   G3DSA:1.20.840.10
- Gene3D:   G3DSA:3.30.70.470
- PIRSF:   PIRSF000263
- TIGRFAMs:   TIGR03257

Pfam domain/function: PF02241 MCR_beta; PF02783 MCR_beta_N; SSF48081 MCR_alpha_beta_C; SSF55088 MCR_fer_like

EC number: =2.8.4.1

Molecular weight: Translated: 47790; Mature: 47790

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPMVKYKDTINLYDEKGKLVEENVPLEAISPLHNPTIQKLVKDVKRTVAVNLAGIENAL
CCCCEEECCCCCEECCCCCEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHEEHHHHHHHH
RTGQVGGKGCMIKGRELDLPIVENAETIAEYVEKVVRVSEDDDTSIKLINDGKQMAVQIP
HCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECC
SKRLDVAAEYSVSVLVTAQALKEAIIRTFDVDIFDAPMVHAAVLGGYPHEVTMKGSNIAA
CHHHCEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCEEE
LLGSPLSLEGPGYALRNIMANHFVACTKKNVMNAVAFAAIMEQTAMFEMGDAVGLFERLH
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVIKEDKTLPSGFKLYKP
HHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
VDVAKWNAYAAAGLVAAAIVNCGAARAAQNIASTILYYNDILEYETGLPGVDFGRAEGTA
CCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPPVAAAMCLDAGTQMFSPERTSALVG
EEHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHCCCCHHHHHHH
TVFSAIDEFREPLKYVIKGALEVKDKI
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPMVKYKDTINLYDEKGKLVEENVPLEAISPLHNPTIQKLVKDVKRTVAVNLAGIENAL
CCCCEEECCCCCEECCCCCEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHEEHHHHHHHH
RTGQVGGKGCMIKGRELDLPIVENAETIAEYVEKVVRVSEDDDTSIKLINDGKQMAVQIP
HCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECC
SKRLDVAAEYSVSVLVTAQALKEAIIRTFDVDIFDAPMVHAAVLGGYPHEVTMKGSNIAA
CHHHCEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCEEE
LLGSPLSLEGPGYALRNIMANHFVACTKKNVMNAVAFAAIMEQTAMFEMGDAVGLFERLH
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVIKEDKTLPSGFKLYKP
HHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
VDVAKWNAYAAAGLVAAAIVNCGAARAAQNIASTILYYNDILEYETGLPGVDFGRAEGTA
CCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPPVAAAMCLDAGTQMFSPERTSALVG
EEHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHCCCCHHHHHHH
TVFSAIDEFREPLKYVIKGALEVKDKI
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087