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The map label for this gene is prf1 [H]

Identifier: 15669016

GI number: 15669016

Start: 748826

End: 750103

Strand: Direct

Name: prf1 [H]

Synonym: MJ0829

Alternate gene names: 15669016

Gene position: 748826-750103 (Clockwise)

Preceding gene: 15669015

Following gene: 15669018

Centisome position: 44.98

GC content: 35.52

Gene sequence:

>1278_bases
ATGGTGATAGTTATGGCATCAACTGATTCAAAACAATTATATTTATTTAAAAAGATGTTGAAAGAATTAAAATCTAAGAA
AGGTAAGGGGACTGAGCTTATCAGCCTTTATATTCCAGCAGGTAGAAGGATATCTGATGTAGCTCAGCATTTAAGGGAGG
AGATGTCACAGGCATCAAACATTAAAAGTAAAAGCACAAGGAAAAATGTTCAATCAGCAATAGAGGCAATTTTGCAGAGA
TTAAAGTTATTAAAAGAGCCATTAGAGAAAGGAGTTGTTATATTCGCTGGAATGGTTCCAAGAAGTGGGCCTGGAACAGA
AAAGATGGAAACCTACGTTATAGAGCCACCAGAACCAATAAAAACATACATCTATAGATGTGATTCAGAATTTTATTTAG
AGCCGTTAGAGGAATTCTTAGAGGATAAAGATGCGTATGGAGTTATATTGGTTGATAGAAACGAAGCTACAATAGCGTTA
GTTAAAGGTAGAAACATAAATATCTTAAAAAAACTAACAAGTGGAGTTCCTGGAAAGTTTAAAGCAGGAGGGCAGTCAGC
AAGAAGATTAGAAAGGCTTATAGATTTAGCCGCTCATGAGTTCTTGCAGAGAGTTGGGCAGAAGGCAAATGAGCAATTCC
TTCCATTATTGCAAGAGAAAAAACTTAGAGGAATCTTAGTTGGAGGGCCAGGACATACAAAGAATGAGTTTGTTGAAGGA
GATTACTTACACCATGAACTTAAAAAGATTGTATTGGATACATTTGATTTATGCTATACAGAGGAGTTTGGTATAAGAGA
GCTCTTAGAGAAAGCAGCTCCATTATTAAAGGATGTTGAGTTGATGAAAGAGAGAGAAGCTGTTCAGAGATTTTTAAAGG
AATTAATTAAAGAGGATGGAGGATTAGCTTGCTATGGAGAGAAAGAGGTTTTAGAGGCTTTAATGATGGGAGCTGTTGAT
ACTTTAATTGTTTCTGAAGAATTGGAGAAATATAAGGTAAAGATAGCATGTAACAATTGCGATTACTTAGAGGAAAAAAC
TGTCAATAAGCTTGAGCTTATTAAATTGGAAGAAGAGCTTAAAAATGCCCAATGCCCAAAATGTGGAGGAGCTTTAAGTA
TCGTTGAGGAAAAAGACTATATTGAATACCTCTCAGAACTATGTGAGCAGAGCGGGGCTAAACTTGTAACAGTTTCATCA
GAAACAGAAGAAGGGGCTATGATTTTAAATGCATTTAAGGGAATAGCCGCTATATTGAGATATAAGATTCATCAATAA

Upstream 100 bases:

>100_bases
TATTTTACACTCAATATTCATGTCTCATTTTAAAAACACACTAATCATTTTTACGATTTTAAAAATTAGATACCAAAAAT
AAATTTAAAATAAAAGTCTC

Downstream 100 bases:

>100_bases
CTCCTGTATATCTCTAAATTTATGCATACTTTTTTAAATCCATAATCCTTAAATTTTTTAATAATTTCTTCTTTTTCATT
AATTATCTTACTTAAATCAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MVIVMASTDSKQLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASNIKSKSTRKNVQSAIEAILQR
LKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPIKTYIYRCDSEFYLEPLEEFLEDKDAYGVILVDRNEATIAL
VKGRNINILKKLTSGVPGKFKAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLRGILVGGPGHTKNEFVEG
DYLHHELKKIVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKEREAVQRFLKELIKEDGGLACYGEKEVLEALMMGAVD
TLIVSEELEKYKVKIACNNCDYLEEKTVNKLELIKLEEELKNAQCPKCGGALSIVEEKDYIEYLSELCEQSGAKLVTVSS
ETEEGAMILNAFKGIAAILRYKIHQ

Sequences:

>Translated_425_residues
MVIVMASTDSKQLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASNIKSKSTRKNVQSAIEAILQR
LKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPIKTYIYRCDSEFYLEPLEEFLEDKDAYGVILVDRNEATIAL
VKGRNINILKKLTSGVPGKFKAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLRGILVGGPGHTKNEFVEG
DYLHHELKKIVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKEREAVQRFLKELIKEDGGLACYGEKEVLEALMMGAVD
TLIVSEELEKYKVKIACNNCDYLEEKTVNKLELIKLEEELKNAQCPKCGGALSIVEEKDYIEYLSELCEQSGAKLVTVSS
ETEEGAMILNAFKGIAAILRYKIHQ
>Mature_425_residues
MVIVMASTDSKQLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASNIKSKSTRKNVQSAIEAILQR
LKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPIKTYIYRCDSEFYLEPLEEFLEDKDAYGVILVDRNEATIAL
VKGRNINILKKLTSGVPGKFKAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLRGILVGGPGHTKNEFVEG
DYLHHELKKIVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKEREAVQRFLKELIKEDGGLACYGEKEVLEALMMGAVD
TLIVSEELEKYKVKIACNNCDYLEEKTVNKLELIKLEEELKNAQCPKCGGALSIVEEKDYIEYLSELCEQSGAKLVTVSS
ETEEGAMILNAFKGIAAILRYKIHQ

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=419, Percent_Identity=32.2195704057279, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI72000368, Length=415, Percent_Identity=31.3253012048193, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6319619, Length=419, Percent_Identity=32.6968973747017, Blast_Score=206, Evalue=8e-54,
Organism=Drosophila melanogaster, GI28574632, Length=415, Percent_Identity=32.0481927710843, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI21356433, Length=415, Percent_Identity=32.0481927710843, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24667392, Length=415, Percent_Identity=32.0481927710843, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24667388, Length=415, Percent_Identity=32.0481927710843, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24667380, Length=415, Percent_Identity=32.0481927710843, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24667384, Length=415, Percent_Identity=32.0481927710843, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 47952; Mature: 47952

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIVMASTDSKQLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASN
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHC
IKSKSTRKNVQSAIEAILQRLKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEECCCCHH
KTYIYRCDSEFYLEPLEEFLEDKDAYGVILVDRNEATIALVKGRNINILKKLTSGVPGKF
HHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCC
KAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLRGILVGGPGHTKNEFVEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
DYLHHELKKIVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKEREAVQRFLKELIKEDG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLACYGEKEVLEALMMGAVDTLIVSEELEKYKVKIACNNCDYLEEKTVNKLELIKLEEEL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHCCCHHHHHHHHHHH
KNAQCPKCGGALSIVEEKDYIEYLSELCEQSGAKLVTVSSETEEGAMILNAFKGIAAILR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
YKIHQ
HHCCC
>Mature Secondary Structure
MVIVMASTDSKQLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASN
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHC
IKSKSTRKNVQSAIEAILQRLKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEECCCCHH
KTYIYRCDSEFYLEPLEEFLEDKDAYGVILVDRNEATIALVKGRNINILKKLTSGVPGKF
HHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCC
KAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLRGILVGGPGHTKNEFVEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
DYLHHELKKIVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKEREAVQRFLKELIKEDG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLACYGEKEVLEALMMGAVDTLIVSEELEKYKVKIACNNCDYLEEKTVNKLELIKLEEEL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHCCCHHHHHHHHHHH
KNAQCPKCGGALSIVEEKDYIEYLSELCEQSGAKLVTVSSETEEGAMILNAFKGIAAILR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
YKIHQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 10788613 [H]