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The map label for this gene is cbiH

Identifier: 15669000

GI number: 15669000

Start: 736810

End: 737559

Strand: Reverse

Name: cbiH

Synonym: MJ0813

Alternate gene names: 15669000

Gene position: 737559-736810 (Counterclockwise)

Preceding gene: 15669001

Following gene: 15668999

Centisome position: 44.3

GC content: 31.07

Gene sequence:

>750_bases
ATGCTGTATGTTGTAGGTATAGGAAGCGGTAATGAGAGGCATTTTACAAAAGAGGCTGAGGAGATTTTAAATAAAGTGGA
TTTAATAGTGTGTTATAAAAATTACAAAAAGTTTGTTGAGAGGCTTAACAAGCCAATATATACAACTGGAATGACAAGGG
AAATTGATAGAGTTGATTATGCCTTAAAAGAGGCTAAAGATAAAGATGTTGCATTAGTTTCAAGTGGTGATGCAACAATT
TATGGCTTAGCTTCGTTAGCTTATGAGATAAACGCAGTTAAAGGTTATAACGTAGATATAAAGGTTGTTCCAGGGATAAC
CGCATGTTCATTAGCTTCAGCAATCTTAGGAAGTCCGTTAAATCATGATTTTGTTGTTATAAGCTTTAGTGATTTATTAA
CCCCTTTAGAGACAATATTAAAGAGGTTTAGATGTGCGTTAGAGGGAGATTTTGTTATATGCATATACAATCCACTAAGT
AAAAGGAGGAAAGAACCATTCTTAAAAGCTATGGAAATATTGGCTGAGTTTGCAAAGGATAAAGATTATATAATTGGGAT
AGTTAAAAATGCTGGTAGAAATAAAGAAGAAGTTGTAATTACAAACTTCAAAGATCTTTATAAAAACTTAGAAAAATACT
TGGAGTTTATAGACATGAATACAATATTAATCATTGGTAATTCTTCAACAAAGATTATCAATGGCAAGATGATTACACCA
AGAGGCTATTTAGATAAATATAAAATTTAG

Upstream 100 bases:

>100_bases
TCGTGGGGAAAAATTGGGGCTAAGGCGGATTATGTTGAAATTTGGGGAGATGCAACAATAATATTCCCATTATTGGTTTA
TTGTGTGATGAAGTGATAGT

Downstream 100 bases:

>100_bases
GTGAAAAATTATGCTTGAAAAAATCAGAGAGGAGTTAAACTCATATTTTTTAGAAAGGAGGGAGGAGATTGATATTGCTT
TAACTTCAATCTTGGCTAAT

Product: cobalamin biosynthesis precorrin-3 methylase CbiH

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATI
YGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLS
KRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITP
RGYLDKYKI

Sequences:

>Translated_249_residues
MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATI
YGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLS
KRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITP
RGYLDKYKI
>Mature_249_residues
MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATI
YGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLS
KRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITP
RGYLDKYKI

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIH_METJA (Q58223)

Other databases:

- EMBL:   L77117
- PIR:   E64401
- RefSeq:   NP_247804.1
- ProteinModelPortal:   Q58223
- GeneID:   1451696
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0813
- NMPDR:   fig|243232.1.peg.841
- TIGR:   MJ0813
- HOGENOM:   HBG305606
- OMA:   EATPEMA
- ProtClustDB:   CLSK876341
- BioCyc:   MJAN243232:MJ_0813-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- TIGRFAMs:   TIGR01466

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: 2.1.1.131

Molecular weight: Translated: 28131; Mature: 28131

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDY
CEEEEEECCCCCCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCEEECCCCHHHHHHHH
ALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPL
HHHHCCCCCEEEEECCCCEEEEHHHHHEEEECEECCCEEEEEECCCHHHHHHHHHHCCCC
NHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKD
CCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCHHHHHHHHHHHHHCC
KDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITP
CCEEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECEEECC
RGYLDKYKI
CCCCCCCCC
>Mature Secondary Structure
MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDY
CEEEEEECCCCCCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCEEECCCCHHHHHHHH
ALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPL
HHHHCCCCCEEEEECCCCEEEEHHHHHEEEECEECCCEEEEEECCCHHHHHHHHHHCCCC
NHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKD
CCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCHHHHHHHHHHHHHCC
KDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITP
CCEEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECEEECC
RGYLDKYKI
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087