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The map label for this gene is leuB

Identifier: 15668901

GI number: 15668901

Start: 653926

End: 654927

Strand: Reverse

Name: leuB

Synonym: MJ0720

Alternate gene names: 15668901

Gene position: 654927-653926 (Counterclockwise)

Preceding gene: 15668903

Following gene: 15668900

Centisome position: 39.34

GC content: 35.23

Gene sequence:

>1002_bases
ATGCATAAAATATGTGTTATAGAAGGAGATGGAATTGGTAAAGAGGTTGTTCCAGCAACAATTCAAGTTTTAGAAGCTAC
TGGTTTGCCATTTGAGTTTGTCTATGCTGAGGCAGGGGATGAGGTTTATAAAAGAACTGGTAAGGCATTACCAGAAGAAA
CAATTGAAACTGCCTTAGACTGTGATGCTGTTTTATTTGGAGCGGCTGGAGAAACAGCGGCAGATGTTATTGTTAAATTG
AGGCATATATTGGATACTTATGCAAACATTAGACCAGTTAAAGCATACAAAGGAGTTAAGTGCCTAAGGCCAGATATTGA
TTACGTTATAGTTAGGGAAAACACTGAAGGGCTTTATAAAGGAATAGAGGCAGAGATTGATGAAGGAATTACAATAGCTA
CAAGAGTTATAACAGAAAAAGCATGTGAGAGAATATTTAGATTTGCTTTTAACTTAGCAAGGGAAAGAAAGAAGATGGGC
AAAGAAGGAAAGGTTACATGTGCTCACAAAGCAAATGTCTTAAAATTAACTGATGGGTTATTTAAAAAGATATTTTATAA
AGTTGCAGAGGAATATGACGATATAAAAGCAGAAGATTATTACATAGATGCAATGAATATGTATATCATAACAAAACCGC
AAGTATTTGATGTTGTAGTTACTTCCAACTTATTTGGAGATATTTTATCAGATGGAGCTGCTGGAACTGTTGGGGGATTA
GGTTTAGCTCCTTCAGCGAATATAGGAGATGAACATGGATTATTTGAGCCGGTTCATGGTTCAGCTCCAGATATTGCTGG
AAAAAAGATAGCTAATCCAACAGCTACAATATTAAGTGCTGTTTTAATGCTTAGATACTTAGGAGAGTATGAAGCTGCAG
ATAAAGTTGAAAAAGCATTGGAGGAAGTTTTAGCTTTAGGTTTAACAACACCTGACTTAGGAGGTAATTTAAATACATTT
GAAATGGCTGAAGAAGTAGCTAAAAGAGTAAGAGAAGAATAA

Upstream 100 bases:

>100_bases
ATTTTAATCATTTTTGATATGGTTTAAGTCACCAACAAAACATTTTTATGTGAAAGTATTTTTATTAATATTGCTATAAT
TAATCTTTTTGGTGATAAGT

Downstream 100 bases:

>100_bases
ATTAATCTATTTTTCTTTAGAAAGCTTTTCTATTCTTTTATTTTAAAAATTTAAAATGAAATTAGGTTTTTATTTATTAG
GAGGTGATTTTATGAGATTG

Product: multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase

Products: NA

Alternate protein names: 3-isopropylmalate dehydrogenase; 3-IPM-DH; IMDH; IPMDH; Beta-IPM dehydrogenase; D-malate dehydrogenase [decarboxylating]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALDCDAVLFGAAGETAADVIVKL
RHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENTEGLYKGIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMG
KEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAGTVGGL
GLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADKVEKALEEVLALGLTTPDLGGNLNTF
EMAEEVAKRVREE

Sequences:

>Translated_333_residues
MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALDCDAVLFGAAGETAADVIVKL
RHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENTEGLYKGIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMG
KEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAGTVGGL
GLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADKVEKALEEVLALGLTTPDLGGNLNTF
EMAEEVAKRVREE
>Mature_333_residues
MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALDCDAVLFGAAGETAADVIVKL
RHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENTEGLYKGIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMG
KEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAGTVGGL
GLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADKVEKALEEVLALGLTTPDLGGNLNTF
EMAEEVAKRVREE

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmal

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=39.8826979472141, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=35.4166666666667, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI28178838, Length=304, Percent_Identity=35.8552631578947, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI28178816, Length=333, Percent_Identity=34.2342342342342, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI28178821, Length=333, Percent_Identity=34.2342342342342, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI28178819, Length=202, Percent_Identity=37.6237623762376, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=40.7303370786517, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=38.2920110192837, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1787381, Length=372, Percent_Identity=32.7956989247312, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=38.0116959064327, Blast_Score=223, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17505779, Length=328, Percent_Identity=37.8048780487805, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=35.7771260997067, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=36.2068965517241, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=46.264367816092, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=36.9369369369369, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6324709, Length=338, Percent_Identity=36.094674556213, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24643270, Length=336, Percent_Identity=40.1785714285714, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24643268, Length=336, Percent_Identity=40.1785714285714, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24661184, Length=341, Percent_Identity=37.5366568914956, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=33.1378299120235, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=33.1378299120235, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI161078637, Length=341, Percent_Identity=33.1378299120235, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=33.1378299120235, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=33.2344213649852, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI281362242, Length=328, Percent_Identity=34.1463414634146, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24648872, Length=328, Percent_Identity=34.1463414634146, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI20130355, Length=332, Percent_Identity=29.8192771084337, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45550436, Length=242, Percent_Identity=25.6198347107438, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_METJA (Q58130)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247705.1
- ProteinModelPortal:   Q58130
- SMR:   Q58130
- GeneID:   1451597
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0720
- NMPDR:   fig|243232.1.peg.742
- TIGR:   MJ0720
- HOGENOM:   HBG518924
- OMA:   TCAHKAN
- ProtClustDB:   PRK14025
- BioCyc:   MetaCyc:MONOMER-13649
- BioCyc:   MJAN243232:MJ_0720-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- TIGRFAMs:   TIGR02088

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85; =1.1.1.83

Molecular weight: Translated: 36330; Mature: 36330

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 81-81 BINDING 91-91 BINDING 112-112 BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALD
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHC
CDAVLFGAAGETAADVIVKLRHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENTEGLYK
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEECCCCCEEEEECCCHHHHH
GIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMGKEGKVTCAHKANVLKLTDGL
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHH
FKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAGTVGGL
HHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCCCCC
GLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADKVEKAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
EEVLALGLTTPDLGGNLNTFEMAEEVAKRVREE
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALD
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHC
CDAVLFGAAGETAADVIVKLRHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENTEGLYK
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEECCCCCEEEEECCCHHHHH
GIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMGKEGKVTCAHKANVLKLTDGL
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHH
FKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAGTVGGL
HHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCCCCC
GLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADKVEKAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
EEVLALGLTTPDLGGNLNTFEMAEEVAKRVREE
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051