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The map label for this gene is hypF

Identifier: 15668895

GI number: 15668895

Start: 646079

End: 648379

Strand: Reverse

Name: hypF

Synonym: MJ0713

Alternate gene names: 15668895

Gene position: 648379-646079 (Counterclockwise)

Preceding gene: 15668898

Following gene: 15668894

Centisome position: 38.94

GC content: 31.77

Gene sequence:

>2301_bases
ATGGAGATATTATTTTTTGGTGTTAAAATGAAGGTAAGAATTAAAGTTAAAGGTATAGTTCAAGGTGTAGGGTTTAGACC
TTTTGTTTATAGAATAGCTAAAAAAAATAATTTAAAGGGCTATGTAAAAAACATGGGGAATTATGTGGAAATTCTTATTG
AAGGAAAAAAAGAGGATATAAGAAATTTTATCAATGATTTAAAAAATAAGAAACCGCCATTGTCAAGAATTGATAAATTG
GATATTGAGGAAATTAAAGGAATTGAAGAATTTGATGACTTCTATATTATAAAGAGTGAAAACGCTAAAGATGAGGAAGA
AGGCACTATACCAGCTGATGTAGCAATATGTGACGACTGCTTAAAAGAAATGCTTGATAAAAATGATAGGAGATACAGAT
ACCCATTTATTGCATGCACAAATTGTGGGCCGAGATTTACAATAGTTGAAAAACTTCCCTATGATAGAGAAAATACATCA
ATGAGAGATTTTCCTTTATGTGAAAAGTGCTTGGAGGAATATAAAAATCCTTTAGATAGGAGATTTCATGCTCAAGCCAC
TTGTTGCCCAATTTGCGGTCCTAAGGTATTTTTGAGTGATGGAAAAGAGATTATAGCTGAAAAAGATGAAGCAATTAGAG
AAACAGTTAAATTATTGGAAGAGGGTCATATATTAGCTATAAAAGGAATTGGAGGGACTCACTTAGCTTGTAAAGTAGGA
GAGGATGATGTAGTTTTAGAATTAAGGAAGAGATTGGGAAGACCAACTCAACCATTTGCAGTAATGAGTAAAATAGAATA
TACAGAGCTGTTTGCTGAATTTGACGAAGATGAAAAAAATGCTTTGTTATCTTTAAGAAGACCAATAGTTGTTTTAAAAA
AGAGCCAAGATTATGATAAATATTTTTCAAAGTATGTTTCTAATTTAGACACTATTGGAGTTATGTTTCCATACAGTGGG
TTGCATTATCTCTTATTTGATAAAGAGATTGCTTATGTTATGACCTCTGCTAATCTGCCAGGATTACCAATGGTTAAGGA
TAATGATGAGATATTAAAAAAACTTAACGGTATTGCTGACTACTTCTTATTGCATAATAGAAGGATAGTGAATAGATGTG
ATGACAGTGTTGTTAAAAAGGTAGCTGATAGATTAGTTTTTTTAAGGAGGTCAAGGGGATTTGCTCCAGAGCCTGTAAAG
GTTAATATAAACAATAATAAAAATATCCTATGTGTTGGGGCTGAGCTAAACTCAACCGCTTGTATTGTAAAGAGAGATAA
GTTTTATCTAACCCAGTATATAGGAAATACCTCAAAGTATGAGACATTCTGCTATCTAAGAGATGCGATAAACAACATTT
TAAGATTAACAAACACAAATAAAATTGATGCTATTGTTTGTGACTTGCATCCTCAGTTTAATTCAACAAAATTAGCTGAG
GAATTATCAGAAAAATTTGGGGCTGAGATTTTTAGAGTTCAGCATCATTTTGCACATGCTTATAGCTTATTAGGGGACAA
CAACTATTTCGATGATGCAATAATTTTGTCGTTGGATGGGGTAGGTTATGGATTGGATGGGAATATTTGGGGAGGGGAGG
TTTTGTTATTTAAAGATGGCAAGATGGAGAGAGTAGGGCATTTGGAGGAACAGTATCAGTTAGGAGGGGACTTAGCAACT
AAGTATCCTTTGAGGATGCTACTTTCTATATTATATAAAGCCATTGGTGAGGAGGCATTTGATTTTATAAAAAGATATAA
TTTCTTCTCAGAAAAAGAACTTAGATTATTAAAATTCCAACTTGAGAAAAAACTTAACTGTCCAATAACTACATCCACTG
GTAGAGTTTTAGATGCTGTTTCAGCTTTATTAGGAATTTGCTTTGAAAAAACTTACGATGGAGAGCCGAGTATAAGATTA
GAGCCAGTGGCAAATAGGTTTAAAGGAGATATTAATATAGAGCCAAAAATAAAAAATAACATCTTAAATACTACAGAACT
TATTTACAAATCTTATGAGATGCTATTAAATAACGAAAATAAAGAAAAAATAGCACATTTTGCCCATATTTATATAGCAG
ATGGATTATTTGAGATTGCTAAGAAAATATCGAATAAATTTGGAATAAATACTATAGGCATTACTGGAGGAGTCTCATAT
AACAAAATAATAACTGAAAGAATTATGAATAATGCAAAAAGGGAGGGTTTTAATTTTATTTATCATCAAAGAGTTCCTAA
TGGAGATGGGGGAATTAGTTTTGGGCAAGGTGTTGCCTATATCTTAAAAAATGGATATTAA

Upstream 100 bases:

>100_bases
ATCATAAAATTTAATATGTATATTAAAAATTTTAATAATATCAATTAGATTTTCAAAACTACATATTAAAAAATTAAATA
ATAATCAAATTTAAAATTAA

Downstream 100 bases:

>100_bases
TTGGGGCTGAAAGCCCCAACTTAATGGATAACGGGTATCCCAATAGGCAGAGCCCTATGGGGCGGGATTAGTTTTGGACA
AGGAATAGCTTATATTTTAA

Product: hydrogenase expression regulatory protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF

Number of amino acids: Translated: 766; Mature: 766

Protein sequence:

>766_residues
MEILFFGVKMKVRIKVKGIVQGVGFRPFVYRIAKKNNLKGYVKNMGNYVEILIEGKKEDIRNFINDLKNKKPPLSRIDKL
DIEEIKGIEEFDDFYIIKSENAKDEEEGTIPADVAICDDCLKEMLDKNDRRYRYPFIACTNCGPRFTIVEKLPYDRENTS
MRDFPLCEKCLEEYKNPLDRRFHAQATCCPICGPKVFLSDGKEIIAEKDEAIRETVKLLEEGHILAIKGIGGTHLACKVG
EDDVVLELRKRLGRPTQPFAVMSKIEYTELFAEFDEDEKNALLSLRRPIVVLKKSQDYDKYFSKYVSNLDTIGVMFPYSG
LHYLLFDKEIAYVMTSANLPGLPMVKDNDEILKKLNGIADYFLLHNRRIVNRCDDSVVKKVADRLVFLRRSRGFAPEPVK
VNINNNKNILCVGAELNSTACIVKRDKFYLTQYIGNTSKYETFCYLRDAINNILRLTNTNKIDAIVCDLHPQFNSTKLAE
ELSEKFGAEIFRVQHHFAHAYSLLGDNNYFDDAIILSLDGVGYGLDGNIWGGEVLLFKDGKMERVGHLEEQYQLGGDLAT
KYPLRMLLSILYKAIGEEAFDFIKRYNFFSEKELRLLKFQLEKKLNCPITTSTGRVLDAVSALLGICFEKTYDGEPSIRL
EPVANRFKGDINIEPKIKNNILNTTELIYKSYEMLLNNENKEKIAHFAHIYIADGLFEIAKKISNKFGINTIGITGGVSY
NKIITERIMNNAKREGFNFIYHQRVPNGDGGISFGQGVAYILKNGY

Sequences:

>Translated_766_residues
MEILFFGVKMKVRIKVKGIVQGVGFRPFVYRIAKKNNLKGYVKNMGNYVEILIEGKKEDIRNFINDLKNKKPPLSRIDKL
DIEEIKGIEEFDDFYIIKSENAKDEEEGTIPADVAICDDCLKEMLDKNDRRYRYPFIACTNCGPRFTIVEKLPYDRENTS
MRDFPLCEKCLEEYKNPLDRRFHAQATCCPICGPKVFLSDGKEIIAEKDEAIRETVKLLEEGHILAIKGIGGTHLACKVG
EDDVVLELRKRLGRPTQPFAVMSKIEYTELFAEFDEDEKNALLSLRRPIVVLKKSQDYDKYFSKYVSNLDTIGVMFPYSG
LHYLLFDKEIAYVMTSANLPGLPMVKDNDEILKKLNGIADYFLLHNRRIVNRCDDSVVKKVADRLVFLRRSRGFAPEPVK
VNINNNKNILCVGAELNSTACIVKRDKFYLTQYIGNTSKYETFCYLRDAINNILRLTNTNKIDAIVCDLHPQFNSTKLAE
ELSEKFGAEIFRVQHHFAHAYSLLGDNNYFDDAIILSLDGVGYGLDGNIWGGEVLLFKDGKMERVGHLEEQYQLGGDLAT
KYPLRMLLSILYKAIGEEAFDFIKRYNFFSEKELRLLKFQLEKKLNCPITTSTGRVLDAVSALLGICFEKTYDGEPSIRL
EPVANRFKGDINIEPKIKNNILNTTELIYKSYEMLLNNENKEKIAHFAHIYIADGLFEIAKKISNKFGINTIGITGGVSY
NKIITERIMNNAKREGFNFIYHQRVPNGDGGISFGQGVAYILKNGY
>Mature_766_residues
MEILFFGVKMKVRIKVKGIVQGVGFRPFVYRIAKKNNLKGYVKNMGNYVEILIEGKKEDIRNFINDLKNKKPPLSRIDKL
DIEEIKGIEEFDDFYIIKSENAKDEEEGTIPADVAICDDCLKEMLDKNDRRYRYPFIACTNCGPRFTIVEKLPYDRENTS
MRDFPLCEKCLEEYKNPLDRRFHAQATCCPICGPKVFLSDGKEIIAEKDEAIRETVKLLEEGHILAIKGIGGTHLACKVG
EDDVVLELRKRLGRPTQPFAVMSKIEYTELFAEFDEDEKNALLSLRRPIVVLKKSQDYDKYFSKYVSNLDTIGVMFPYSG
LHYLLFDKEIAYVMTSANLPGLPMVKDNDEILKKLNGIADYFLLHNRRIVNRCDDSVVKKVADRLVFLRRSRGFAPEPVK
VNINNNKNILCVGAELNSTACIVKRDKFYLTQYIGNTSKYETFCYLRDAINNILRLTNTNKIDAIVCDLHPQFNSTKLAE
ELSEKFGAEIFRVQHHFAHAYSLLGDNNYFDDAIILSLDGVGYGLDGNIWGGEVLLFKDGKMERVGHLEEQYQLGGDLAT
KYPLRMLLSILYKAIGEEAFDFIKRYNFFSEKELRLLKFQLEKKLNCPITTSTGRVLDAVSALLGICFEKTYDGEPSIRL
EPVANRFKGDINIEPKIKNNILNTTELIYKSYEMLLNNENKEKIAHFAHIYIADGLFEIAKKISNKFGINTIGITGGVSY
NKIITERIMNNAKREGFNFIYHQRVPNGDGGISFGQGVAYILKNGY

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain

Homologues:

Organism=Escherichia coli, GI2367152, Length=769, Percent_Identity=35.5006501950585, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPF_METJA (Q58123)

Other databases:

- EMBL:   L77117
- PIR:   A64389
- RefSeq:   NP_247698.1
- ProteinModelPortal:   Q58123
- SMR:   Q58123
- GeneID:   1451591
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0713
- NMPDR:   fig|243232.1.peg.735
- TIGR:   MJ0713
- HOGENOM:   HBG287964
- OMA:   TIAERLP
- ProtClustDB:   CLSK876302
- BioCyc:   MJAN243232:MJ_0713-MONOMER
- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125
- Gene3D:   G3DSA:3.90.870.10
- PANTHER:   PTHR10029
- PIRSF:   PIRSF006256
- TIGRFAMs:   TIGR00143

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF; SSF54975 Acylphosphatase; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: NA

Molecular weight: Translated: 87606; Mature: 87606

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00150 ACYLPHOSPHATASE_1; PS00151 ACYLPHOSPHATASE_2; PS51160 ACYLPHOSPHATASE_3; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILFFGVKMKVRIKVKGIVQGVGFRPFVYRIAKKNNLKGYVKNMGNYVEILIEGKKEDI
CEEEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEECCHHHH
RNFINDLKNKKPPLSRIDKLDIEEIKGIEEFDDFYIIKSENAKDEEEGTIPADVAICDDC
HHHHHHHCCCCCCHHHHCCCCHHHHCCCHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
LKEMLDKNDRRYRYPFIACTNCGPRFTIVEKLPYDRENTSMRDFPLCEKCLEEYKNPLDR
HHHHHCCCCCEEECCEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCHHHH
RFHAQATCCPICGPKVFLSDGKEIIAEKDEAIRETVKLLEEGHILAIKGIGGTHLACKVG
HHCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEC
EDDVVLELRKRLGRPTQPFAVMSKIEYTELFAEFDEDEKNALLSLRRPIVVLKKSQDYDK
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCHHH
YFSKYVSNLDTIGVMFPYSGLHYLLFDKEIAYVMTSANLPGLPMVKDNDEILKKLNGIAD
HHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
YFLLHNRRIVNRCDDSVVKKVADRLVFLRRSRGFAPEPVKVNINNNKNILCVGAELNSTA
HHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCE
CIVKRDKFYLTQYIGNTSKYETFCYLRDAINNILRLTNTNKIDAIVCDLHPQFNSTKLAE
EEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHH
ELSEKFGAEIFRVQHHFAHAYSLLGDNNYFDDAIILSLDGVGYGLDGNIWGGEVLLFKDG
HHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECC
KMERVGHLEEQYQLGGDLATKYPLRMLLSILYKAIGEEAFDFIKRYNFFSEKELRLLKFQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHH
LEKKLNCPITTSTGRVLDAVSALLGICFEKTYDGEPSIRLEPVANRFKGDINIEPKIKNN
HHHCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCCCCCCCCCHHCC
ILNTTELIYKSYEMLLNNENKEKIAHFAHIYIADGLFEIAKKISNKFGINTIGITGGVSY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCH
NKIITERIMNNAKREGFNFIYHQRVPNGDGGISFGQGVAYILKNGY
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MEILFFGVKMKVRIKVKGIVQGVGFRPFVYRIAKKNNLKGYVKNMGNYVEILIEGKKEDI
CEEEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEECCHHHH
RNFINDLKNKKPPLSRIDKLDIEEIKGIEEFDDFYIIKSENAKDEEEGTIPADVAICDDC
HHHHHHHCCCCCCHHHHCCCCHHHHCCCHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
LKEMLDKNDRRYRYPFIACTNCGPRFTIVEKLPYDRENTSMRDFPLCEKCLEEYKNPLDR
HHHHHCCCCCEEECCEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCHHHH
RFHAQATCCPICGPKVFLSDGKEIIAEKDEAIRETVKLLEEGHILAIKGIGGTHLACKVG
HHCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEC
EDDVVLELRKRLGRPTQPFAVMSKIEYTELFAEFDEDEKNALLSLRRPIVVLKKSQDYDK
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCHHH
YFSKYVSNLDTIGVMFPYSGLHYLLFDKEIAYVMTSANLPGLPMVKDNDEILKKLNGIAD
HHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
YFLLHNRRIVNRCDDSVVKKVADRLVFLRRSRGFAPEPVKVNINNNKNILCVGAELNSTA
HHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCE
CIVKRDKFYLTQYIGNTSKYETFCYLRDAINNILRLTNTNKIDAIVCDLHPQFNSTKLAE
EEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHH
ELSEKFGAEIFRVQHHFAHAYSLLGDNNYFDDAIILSLDGVGYGLDGNIWGGEVLLFKDG
HHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECC
KMERVGHLEEQYQLGGDLATKYPLRMLLSILYKAIGEEAFDFIKRYNFFSEKELRLLKFQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHH
LEKKLNCPITTSTGRVLDAVSALLGICFEKTYDGEPSIRLEPVANRFKGDINIEPKIKNN
HHHCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCCCCCCCCCHHCC
ILNTTELIYKSYEMLLNNENKEKIAHFAHIYIADGLFEIAKKISNKFGINTIGITGGVSY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCH
NKIITERIMNNAKREGFNFIYHQRVPNGDGGISFGQGVAYILKNGY
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087