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The map label for this gene is cobQ [H]

Identifier: 15668661

GI number: 15668661

Start: 425552

End: 427039

Strand: Direct

Name: cobQ [H]

Synonym: MJ0484

Alternate gene names: 15668661

Gene position: 425552-427039 (Clockwise)

Preceding gene: 15668659

Following gene: 15668662

Centisome position: 25.56

GC content: 31.72

Gene sequence:

>1488_bases
TTGATGAAGATGGCAGAGTTTATAATGGTTGTTGGAACATCATCAAATAGTGGAAAAACGACAATAACTGCTGGATTATG
CAGAATTTTAGCAAATAAAGGCTATAAAGTAGCCCCATTCAAATCTCAAAATATGAGTTTGAATTCAAGAGTTGCAAAGG
AAGATGGGGAGATTGCTATAGCCCAATACACTCAAAGTTTAGCTTGTAGGGTAGAGCCATCAGTTCATTTTAACCCAATT
TTATTAAAACCAAAAGGTAATTTTATCTCTCAAGTTATAGTCCATGGAAGACCCTACAAAGACATGAATTATAATGAATA
TAGAAAAAATAAAGATTTTTTCTTAAAGAAGATTAAAGAGAGTTTGGAAATTTTAGACAGAGAGTATGATTATGTTATTA
TGGAGGGAGCTGGGAGTTGTTGTGAAATAAATTTATTGAAGGATGATATAGCAAATTTAAGGATAGCTGAGCTTGTAAAT
GCCAAAGCTATTTTGGTTGCAGACATTGATAGGGGTGGAGTATTTGCCTCAATATATGGGACAATAAAACTATTGCCTGA
AAATTGGAGGAAGCTAATTAAAGGAATTATAATAAACAAATTTAGAGGGAATGTAGAGGTTTTAAAGGAAGGGATTGAAA
AAATAGAGGAGCTAACTGGTATTCCAGTTTTAGGCATAGTTCCCTATGATGAAAACCTTGTTTTACCAGAGGAGGATAGT
CAAGTCCTACAGAGCATGAGAAGTTTTGGAAATGCAAAAAGTGGAGTGGAAATTAATGTAGTTAGGTTTTCAAAGATATC
AAACTTTACAGACTTAGACCCATTAAGATACGATGCATTTATAAAGTTTATCGACTTTGATGATGACATAACTGGAGATA
TCTTAATATTTCCGGGAACAAGAAGTTCAACAAAAGAAGCTTATTATTTAAAACAACATAACTTTGATGAAAAGGTTTTG
GAGTTTTTGAAAGATGGGGGAATTGTTATTGGTATCTGTGGAGGTTATCAAGTTTTAGGAAAAGAGTTGATTGATAAAGA
GAAGAAAGAGTCAGATGTTGGAGATATTGAGGGCTTAAAAATCTTTGATGCAAAAACATACTTTGGAAATGATAAAGTAG
TTAAAAACTCTTGTGGTTTCTTAGAGATTGATAATAAAACATTTAATGTTAAAGGCTATGAGATACATGAAGGCTTTACC
TATTCAAAAGAAAAACCTCTCATAAAAATTGAGAGAGGCTTTGGAAACTGTGGAAATGGTTTTGATGGTTCTATTAAAAA
ATTTGGAGATGGATTGGCTATAGGAACATACTTCCATGGAATATTTGAAAATTATGAGTTTAGAAATTATATCATTAATT
TAATTAGAAAAAGGAAAGGTTTGGATGAGATTTATGGAGATTCATACAAAGATAGCATAGAAAAAAGTTTAAATTACTTT
GCTGAAGTTGTAGAGAGAAGTGTTAATTTAAAGCCATTGGGCATTTAA

Upstream 100 bases:

>100_bases
ATACTCTAAACAAAATTAAAAAATCTAATATCAATATTAAAAATCCAATAAACTAAACTAAATATAATCAAATCTCAAAT
AAACTATAAAAAATGCTGAG

Downstream 100 bases:

>100_bases
ATGAAATGAAATTAGAATAATTATAAAATAAAAAAACAAACTGGGATAAAAATGGTAGAGATTAACTTAAGAGAATTAAA
AAAATATGCAAATCCATTCT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MMKMAEFIMVVGTSSNSGKTTITAGLCRILANKGYKVAPFKSQNMSLNSRVAKEDGEIAIAQYTQSLACRVEPSVHFNPI
LLKPKGNFISQVIVHGRPYKDMNYNEYRKNKDFFLKKIKESLEILDREYDYVIMEGAGSCCEINLLKDDIANLRIAELVN
AKAILVADIDRGGVFASIYGTIKLLPENWRKLIKGIIINKFRGNVEVLKEGIEKIEELTGIPVLGIVPYDENLVLPEEDS
QVLQSMRSFGNAKSGVEINVVRFSKISNFTDLDPLRYDAFIKFIDFDDDITGDILIFPGTRSSTKEAYYLKQHNFDEKVL
EFLKDGGIVIGICGGYQVLGKELIDKEKKESDVGDIEGLKIFDAKTYFGNDKVVKNSCGFLEIDNKTFNVKGYEIHEGFT
YSKEKPLIKIERGFGNCGNGFDGSIKKFGDGLAIGTYFHGIFENYEFRNYIINLIRKRKGLDEIYGDSYKDSIEKSLNYF
AEVVERSVNLKPLGI

Sequences:

>Translated_495_residues
MMKMAEFIMVVGTSSNSGKTTITAGLCRILANKGYKVAPFKSQNMSLNSRVAKEDGEIAIAQYTQSLACRVEPSVHFNPI
LLKPKGNFISQVIVHGRPYKDMNYNEYRKNKDFFLKKIKESLEILDREYDYVIMEGAGSCCEINLLKDDIANLRIAELVN
AKAILVADIDRGGVFASIYGTIKLLPENWRKLIKGIIINKFRGNVEVLKEGIEKIEELTGIPVLGIVPYDENLVLPEEDS
QVLQSMRSFGNAKSGVEINVVRFSKISNFTDLDPLRYDAFIKFIDFDDDITGDILIFPGTRSSTKEAYYLKQHNFDEKVL
EFLKDGGIVIGICGGYQVLGKELIDKEKKESDVGDIEGLKIFDAKTYFGNDKVVKNSCGFLEIDNKTFNVKGYEIHEGFT
YSKEKPLIKIERGFGNCGNGFDGSIKKFGDGLAIGTYFHGIFENYEFRNYIINLIRKRKGLDEIYGDSYKDSIEKSLNYF
AEVVERSVNLKPLGI
>Mature_495_residues
MMKMAEFIMVVGTSSNSGKTTITAGLCRILANKGYKVAPFKSQNMSLNSRVAKEDGEIAIAQYTQSLACRVEPSVHFNPI
LLKPKGNFISQVIVHGRPYKDMNYNEYRKNKDFFLKKIKESLEILDREYDYVIMEGAGSCCEINLLKDDIANLRIAELVN
AKAILVADIDRGGVFASIYGTIKLLPENWRKLIKGIIINKFRGNVEVLKEGIEKIEELTGIPVLGIVPYDENLVLPEEDS
QVLQSMRSFGNAKSGVEINVVRFSKISNFTDLDPLRYDAFIKFIDFDDDITGDILIFPGTRSSTKEAYYLKQHNFDEKVL
EFLKDGGIVIGICGGYQVLGKELIDKEKKESDVGDIEGLKIFDAKTYFGNDKVVKNSCGFLEIDNKTFNVKGYEIHEGFT
YSKEKPLIKIERGFGNCGNGFDGSIKKFGDGLAIGTYFHGIFENYEFRNYIINLIRKRKGLDEIYGDSYKDSIEKSLNYF
AEVVERSVNLKPLGI

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 55810; Mature: 55810

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKMAEFIMVVGTSSNSGKTTITAGLCRILANKGYKVAPFKSQNMSLNSRVAKEDGEIAI
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCCCEEE
AQYTQSLACRVEPSVHFNPILLKPKGNFISQVIVHGRPYKDMNYNEYRKNKDFFLKKIKE
EEHHHHHEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHH
SLEILDREYDYVIMEGAGSCCEINLLKDDIANLRIAELVNAKAILVADIDRGGVFASIYG
HHHHHCCCCCEEEEECCCCEEEEEEEHHHHHCEEHHHHCCCCEEEEEECCCCCEEEEEEE
TIKLLPENWRKLIKGIIINKFRGNVEVLKEGIEKIEELTGIPVLGIVPYDENLVLPEEDS
EEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHH
QVLQSMRSFGNAKSGVEINVVRFSKISNFTDLDPLRYDAFIKFIDFDDDITGDILIFPGT
HHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECCC
RSSTKEAYYLKQHNFDEKVLEFLKDGGIVIGICGGYQVLGKELIDKEKKESDVGDIEGLK
CCCCCCEEEEHHCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEE
IFDAKTYFGNDKVVKNSCGFLEIDNKTFNVKGYEIHEGFTYSKEKPLIKIERGFGNCGNG
EEECEEEECCCCEEECCCCEEEECCEEEEEEEEEEECCCCCCCCCCEEEEECCCCCCCCC
FDGSIKKFGDGLAIGTYFHGIFENYEFRNYIINLIRKRKGLDEIYGDSYKDSIEKSLNYF
CCCCHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHH
AEVVERSVNLKPLGI
HHHHHHCCCCEECCC
>Mature Secondary Structure
MMKMAEFIMVVGTSSNSGKTTITAGLCRILANKGYKVAPFKSQNMSLNSRVAKEDGEIAI
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCCCEEE
AQYTQSLACRVEPSVHFNPILLKPKGNFISQVIVHGRPYKDMNYNEYRKNKDFFLKKIKE
EEHHHHHEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHH
SLEILDREYDYVIMEGAGSCCEINLLKDDIANLRIAELVNAKAILVADIDRGGVFASIYG
HHHHHCCCCCEEEEECCCCEEEEEEEHHHHHCEEHHHHCCCCEEEEEECCCCCEEEEEEE
TIKLLPENWRKLIKGIIINKFRGNVEVLKEGIEKIEELTGIPVLGIVPYDENLVLPEEDS
EEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHH
QVLQSMRSFGNAKSGVEINVVRFSKISNFTDLDPLRYDAFIKFIDFDDDITGDILIFPGT
HHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECCC
RSSTKEAYYLKQHNFDEKVLEFLKDGGIVIGICGGYQVLGKELIDKEKKESDVGDIEGLK
CCCCCCEEEEHHCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEE
IFDAKTYFGNDKVVKNSCGFLEIDNKTFNVKGYEIHEGFTYSKEKPLIKIERGFGNCGNG
EEECEEEECCCCEEECCCCEEEECCEEEEEEEEEEECCCCCCCCCCEEEEECCCCCCCCC
FDGSIKKFGDGLAIGTYFHGIFENYEFRNYIINLIRKRKGLDEIYGDSYKDSIEKSLNYF
CCCCHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHH
AEVVERSVNLKPLGI
HHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]