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The map label for this gene is wecC

Identifier: 15668604

GI number: 15668604

Start: 383700

End: 384983

Strand: Reverse

Name: wecC

Synonym: MJ0428

Alternate gene names: 15668604

Gene position: 384983-383700 (Counterclockwise)

Preceding gene: 15668606

Following gene: 15668603

Centisome position: 23.12

GC content: 31.23

Gene sequence:

>1284_bases
ATGACAAAAGTAGAGAAAAATGGCATTGGAAAAAGGATATGTGTAATTGGTTTGGGTTACATTGGTTTGCCAACGGCTTC
AATGTTAGCAATACAGGGATTTGATGTTATTGGTGTGGATATAAATGAAAAAAGAGTGAAAGAAATTAAAGAACTGAGCT
TTAAAACTACAGAAAAAGATTTAATGACTTTAGTTAAAGGGGCTATAAACTCTGGAAATCTAAAAGTGCAAACAAAACCT
GAAAAAGCAGATGTTTTTATTATATGTGTGCCAACACCTTGCATAGAGTGTGATGGAGAAAAAAAATGTGATTTAACCTA
TTTAAATAAAGCTATTGAAAGCATAAAACCATATCTTGAAAATGGGAATTTAATAATAATAGAAAGCACGATTCCTCCAG
GAACAACTGATGATATTTATAAAAAATTATCAAAGGATAAGAAAATTTATGTTGCTCACTGCCCAGAGAGAGTTTTGCCA
GGCAGTATATTGAAGGAACTTGTTGAAAATGATAGAGTTATTGGAGGAGTTGATGAAAAATCTGCTGAAATGGCAAAAGA
GATTTATGAAACTTTTGTTACTGGAAAGATATATTTAACTGATGCTAAAACAGCAGAGATGGTTAAGTTAATGGAAAATA
CTTATAGAGATGTTAATATTGCCTTAGCCAACGAATTTGCAAAAATTGCAGAGGAAATTGGCATTAATGTTTGGGAAGCA
ATAGAATTAGCCAATAAACATCCAAGAGTAAATATTTTAAAGCCAGGGCCAGGAGTAGGTGGGCATTGTATAAGCATAGA
CCCGTGGTTTATTGTTGAGAAATCAAAGAACGCTAAATTAATAAGAACTGCAAGAGAGTTAAACGACTCTATGCCATTAT
TTGTTGTTGAAAAGATAAAGAAGATTATTAAAAAAGATATTGGAAAAGTGGCAATATTTGGAGTAACATATAAAGGAAAT
GTAGATGACACAAGGGAAAGTCCAGCTGAAAAAGTGGTTAGTAAATTGATAGATGAGGGCTTTGAAGTTAAATGCTATGA
TAAATATGCGAGAGATTTTATTTATCCTTTAAATAGTTTAGATGAAGCTGTTGAAGGAGCTGATATTATCGTTATATTAG
CTGAGCATGATGAATATAAAAATTTTGATAAAGAAGATATAAAAAATATCGCCTCAAAGGTAAAAAATAAAATAATCCTT
GATACTAAAAATATATTAAATAGAGAGTTGTGGGAAAAGGAGGGCTTTAAAGTTTATGTCTTAGGTGATGGAAAGAATGC
ATAA

Upstream 100 bases:

>100_bases
ATTGCTTATAGTTTTGGAGTATATATAACTCACCTATTATATATAAACTGCTAATAATAACTGTCAATAATCAATCTAAA
TAGAAATTTTTGGGGATAAA

Downstream 100 bases:

>100_bases
CCTAAACAATGTCTATCTAAAAGAGTGCATTCACTTCTTAGATAGCTGTATTAACGCACTAAAAGAGTTTGACTTAAGAA
CCTTTATATCGAGATTTTAC

Product: UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTKVEKNGIGKRICVIGLGYIGLPTASMLAIQGFDVIGVDINEKRVKEIKELSFKTTEKDLMTLVKGAINSGNLKVQTKP
EKADVFIICVPTPCIECDGEKKCDLTYLNKAIESIKPYLENGNLIIIESTIPPGTTDDIYKKLSKDKKIYVAHCPERVLP
GSILKELVENDRVIGGVDEKSAEMAKEIYETFVTGKIYLTDAKTAEMVKLMENTYRDVNIALANEFAKIAEEIGINVWEA
IELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIRTARELNDSMPLFVVEKIKKIIKKDIGKVAIFGVTYKGN
VDDTRESPAEKVVSKLIDEGFEVKCYDKYARDFIYPLNSLDEAVEGADIIVILAEHDEYKNFDKEDIKNIASKVKNKIIL
DTKNILNRELWEKEGFKVYVLGDGKNA

Sequences:

>Translated_427_residues
MTKVEKNGIGKRICVIGLGYIGLPTASMLAIQGFDVIGVDINEKRVKEIKELSFKTTEKDLMTLVKGAINSGNLKVQTKP
EKADVFIICVPTPCIECDGEKKCDLTYLNKAIESIKPYLENGNLIIIESTIPPGTTDDIYKKLSKDKKIYVAHCPERVLP
GSILKELVENDRVIGGVDEKSAEMAKEIYETFVTGKIYLTDAKTAEMVKLMENTYRDVNIALANEFAKIAEEIGINVWEA
IELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIRTARELNDSMPLFVVEKIKKIIKKDIGKVAIFGVTYKGN
VDDTRESPAEKVVSKLIDEGFEVKCYDKYARDFIYPLNSLDEAVEGADIIVILAEHDEYKNFDKEDIKNIASKVKNKIIL
DTKNILNRELWEKEGFKVYVLGDGKNA
>Mature_426_residues
TKVEKNGIGKRICVIGLGYIGLPTASMLAIQGFDVIGVDINEKRVKEIKELSFKTTEKDLMTLVKGAINSGNLKVQTKPE
KADVFIICVPTPCIECDGEKKCDLTYLNKAIESIKPYLENGNLIIIESTIPPGTTDDIYKKLSKDKKIYVAHCPERVLPG
SILKELVENDRVIGGVDEKSAEMAKEIYETFVTGKIYLTDAKTAEMVKLMENTYRDVNIALANEFAKIAEEIGINVWEAI
ELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIRTARELNDSMPLFVVEKIKKIIKKDIGKVAIFGVTYKGNV
DDTRESPAEKVVSKLIDEGFEVKCYDKYARDFIYPLNSLDEAVEGADIIVILAEHDEYKNFDKEDIKNIASKVKNKIILD
TKNILNRELWEKEGFKVYVLGDGKNA

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA)

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family

Homologues:

Organism=Homo sapiens, GI4507813, Length=464, Percent_Identity=27.801724137931, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI296040443, Length=453, Percent_Identity=25.1655629139073, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI296040438, Length=366, Percent_Identity=27.0491803278689, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI48994968, Length=408, Percent_Identity=42.6470588235294, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1788340, Length=407, Percent_Identity=25.3071253071253, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560350, Length=469, Percent_Identity=25.7995735607676, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17136908, Length=461, Percent_Identity=25.1626898047722, Blast_Score=121, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECC_METJA (Q57871)

Other databases:

- EMBL:   L77117
- PIR:   D64353
- RefSeq:   NP_247402.1
- ProteinModelPortal:   Q57871
- SMR:   Q57871
- GeneID:   1451288
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0428
- NMPDR:   fig|243232.1.peg.439
- TIGR:   MJ0428
- HOGENOM:   HBG400967
- OMA:   NEVARIC
- ProtClustDB:   CLSK2749882
- BioCyc:   MJAN243232:MJ_0428-MONOMER
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11374
- PIRSF:   PIRSF000124
- TIGRFAMs:   TIGR03026

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N; SSF48179 6DGDH_C_like; SSF52413 UDP-Glc/GDP-Man_DH_C

EC number: 1.1.1.- [C]

Molecular weight: Translated: 47749; Mature: 47618

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: ACT_SITE 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVEKNGIGKRICVIGLGYIGLPTASMLAIQGFDVIGVDINEKRVKEIKELSFKTTEKD
CCCCCCCCCCCEEEEEECCHHCCCCHHHHEECCCEEEEECCCHHHHHHHHHHHCCCCHHH
LMTLVKGAINSGNLKVQTKPEKADVFIICVPTPCIECDGEKKCDLTYLNKAIESIKPYLE
HHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NGNLIIIESTIPPGTTDDIYKKLSKDKKIYVAHCPERVLPGSILKELVENDRVIGGVDEK
CCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEECCCHH
SAEMAKEIYETFVTGKIYLTDAKTAEMVKLMENTYRDVNIALANEFAKIAEEIGINVWEA
HHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCHHHH
IELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIRTARELNDSMPLFVVEKIK
HHHHCCCCEEEEECCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHH
KIIKKDIGKVAIFGVTYKGNVDDTRESPAEKVVSKLIDEGFEVKCYDKYARDFIYPLNSL
HHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHH
DEAVEGADIIVILAEHDEYKNFDKEDIKNIASKVKNKIILDTKNILNRELWEKEGFKVYV
HHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCEEEEECHHHHHHHHHHCCCCEEEE
LGDGKNA
EECCCCC
>Mature Secondary Structure 
TKVEKNGIGKRICVIGLGYIGLPTASMLAIQGFDVIGVDINEKRVKEIKELSFKTTEKD
CCCCCCCCCCEEEEEECCHHCCCCHHHHEECCCEEEEECCCHHHHHHHHHHHCCCCHHH
LMTLVKGAINSGNLKVQTKPEKADVFIICVPTPCIECDGEKKCDLTYLNKAIESIKPYLE
HHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NGNLIIIESTIPPGTTDDIYKKLSKDKKIYVAHCPERVLPGSILKELVENDRVIGGVDEK
CCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEECCCHH
SAEMAKEIYETFVTGKIYLTDAKTAEMVKLMENTYRDVNIALANEFAKIAEEIGINVWEA
HHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCHHHH
IELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIRTARELNDSMPLFVVEKIK
HHHHCCCCEEEEECCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHH
KIIKKDIGKVAIFGVTYKGNVDDTRESPAEKVVSKLIDEGFEVKCYDKYARDFIYPLNSL
HHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHH
DEAVEGADIIVILAEHDEYKNFDKEDIKNIASKVKNKIILDTKNILNRELWEKEGFKVYV
HHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCEEEEECHHHHHHHHHHCCCCEEEE
LGDGKNA
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087