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The map label for this gene is thiI [C]

Identifier: 15668597

GI number: 15668597

Start: 379637

End: 380719

Strand: Direct

Name: thiI [C]

Synonym: MJ0421

Alternate gene names: 15668597

Gene position: 379637-380719 (Clockwise)

Preceding gene: 15668596

Following gene: 15668599

Centisome position: 22.8

GC content: 30.01

Gene sequence:

>1083_bases
ATGAAGTTTATAATAAAAACTCAAAAAGGTTTTGAAAATATCGTTGTGAATAATCTAAAAGAAATTGTTGATGATTTTAA
TTATATCGTTTCTCCTGATGGTTATCAAGGTATTGTTATAGTTGAGAGTGATGAAGATATTGAAGACAAAATACTACAAA
TCCCAGAGGTTGAGAGAGTTTTAAAAGTTTATTTTGAAACAGAAACAGATTTTGATAAAATAGTTAATTTAGCTGAAAAG
ATTAAAGATTACATAAAAGAGGATGAAACTTTTGCTGTAGAAACTAAAAAAAGAGGAAAACATGATTTCAGCTCAACAGA
TATAAACATTGTTTTGGGAGCTAAGATTAAAGATTTAACAAATGCTTCAGTTGATTTAAATAATCCAGATAAGGTTGTTC
ATGTTGAAGTATTTAAAAATAAAACTTATGTATCAATAACCCCTGGAGAAAAGTTCAAAAAATATACCAAGGAGAAAAGA
AATGCGAGAGAGTTATTTAAAAAAGTTGTTATTGTGCAAATGCCATATTTGGGAGAAAAAATTGTTTGTAAAAGGTTTGG
GGAAGCTATTGGGAGAGCAGCTCAAGGATTTGAAGTTAAGGAGTTAATTATAGCACCAAAAGAAAAAGTTGATGCCTATG
AATTGATGGAATTTATTAAAGGAGTTAAGATTGGGCAGCACTCAAGATATGAGATTCAAAAGAGAGCTTATCCATTTGAA
ATTAAGTTAGTTCCAGTGACTGTTCAAGATTTATATCAGGTTGTTAGGGATAAGAGGAGAGATAATAGATTGTTAATAAT
TACTGACCCAAAAGGAGATGAGTTATCAAAAATTAAGGATAAATTAGCTTATGATTTAAGAAAAAAGAGAGAAATTATTG
TATTTTGTGGTTCAAGGGAGGGGATTCCAAGAGGATTATTTAGATTTGCTGACTATATAGTAGATTTAGCTCCACATATG
ACATTTGCTACTGAACATGCTATTCCTGCTGCTTTAATAGCTTTATGGGGAGTTTATAGTGGAGAGGATTTGGAAAATAG
CTCTAAAGAAGAAAAAACAGAATCTAACTCAAATGGTGAATAA

Upstream 100 bases:

>100_bases
CCTTCCTATTGAAGGCGTTCATTAAATACATAATATATTCTAAAATATTTTACCTAAAATATTTTACAAAGAACTATAAA
ACACTTAAAGAGGGATAATC

Downstream 100 bases:

>100_bases
CTTAATCTTTAAATTTTTATTTAATAGGTTGCACTGCACCCACTATGGGGTGCAGTAAAAATATACACCCTACCTACTCT
GAGGTAGGGTTAAAAATAAC

Product: hypothetical protein

Products: ThiS-COSH; AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKFIIKTQKGFENIVVNNLKEIVDDFNYIVSPDGYQGIVIVESDEDIEDKILQIPEVERVLKVYFETETDFDKIVNLAEK
IKDYIKEDETFAVETKKRGKHDFSSTDINIVLGAKIKDLTNASVDLNNPDKVVHVEVFKNKTYVSITPGEKFKKYTKEKR
NARELFKKVVIVQMPYLGEKIVCKRFGEAIGRAAQGFEVKELIIAPKEKVDAYELMEFIKGVKIGQHSRYEIQKRAYPFE
IKLVPVTVQDLYQVVRDKRRDNRLLIITDPKGDELSKIKDKLAYDLRKKREIIVFCGSREGIPRGLFRFADYIVDLAPHM
TFATEHAIPAALIALWGVYSGEDLENSSKEEKTESNSNGE

Sequences:

>Translated_360_residues
MKFIIKTQKGFENIVVNNLKEIVDDFNYIVSPDGYQGIVIVESDEDIEDKILQIPEVERVLKVYFETETDFDKIVNLAEK
IKDYIKEDETFAVETKKRGKHDFSSTDINIVLGAKIKDLTNASVDLNNPDKVVHVEVFKNKTYVSITPGEKFKKYTKEKR
NARELFKKVVIVQMPYLGEKIVCKRFGEAIGRAAQGFEVKELIIAPKEKVDAYELMEFIKGVKIGQHSRYEIQKRAYPFE
IKLVPVTVQDLYQVVRDKRRDNRLLIITDPKGDELSKIKDKLAYDLRKKREIIVFCGSREGIPRGLFRFADYIVDLAPHM
TFATEHAIPAALIALWGVYSGEDLENSSKEEKTESNSNGE
>Mature_360_residues
MKFIIKTQKGFENIVVNNLKEIVDDFNYIVSPDGYQGIVIVESDEDIEDKILQIPEVERVLKVYFETETDFDKIVNLAEK
IKDYIKEDETFAVETKKRGKHDFSSTDINIVLGAKIKDLTNASVDLNNPDKVVHVEVFKNKTYVSITPGEKFKKYTKEKR
NARELFKKVVIVQMPYLGEKIVCKRFGEAIGRAAQGFEVKELIIAPKEKVDAYELMEFIKGVKIGQHSRYEIQKRAYPFE
IKLVPVTVQDLYQVVRDKRRDNRLLIITDPKGDELSKIKDKLAYDLRKKREIIVFCGSREGIPRGLFRFADYIVDLAPHM
TFATEHAIPAALIALWGVYSGEDLENSSKEEKTESNSNGE

Specific function: Required For The Synthesis Of The Thiazole Moiety. [C]

COG id: COG1818

COG function: function code R; Predicted RNA-binding protein, contains THUMP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y421_METJA (Q57864)

Other databases:

- EMBL:   L77117
- PIR:   E64352
- RefSeq:   NP_247395.1
- ProteinModelPortal:   Q57864
- SMR:   Q57864
- GeneID:   1451281
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0421
- NMPDR:   fig|243232.1.peg.432
- TIGR:   MJ0421
- HOGENOM:   HBG297748
- OMA:   ESRYQIQ
- ProtClustDB:   CLSK862764
- BioCyc:   MJAN243232:MJ_0421-MONOMER
- InterPro:   IPR004114
- SMART:   SM00981

Pfam domain/function: PF02926 THUMP

EC number: NA

Molecular weight: Translated: 41397; Mature: 41397

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKTQKGFENIVVNNLKEIVDDFNYIVSPDGYQGIVIVESDEDIEDKILQIPEVERV
CCEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCHHHHHHCCCHHHHH
LKVYFETETDFDKIVNLAEKIKDYIKEDETFAVETKKRGKHDFSSTDINIVLGAKIKDLT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCEEEEECCEEHHCC
NASVDLNNPDKVVHVEVFKNKTYVSITPGEKFKKYTKEKRNARELFKKVVIVQMPYLGEK
CCEEECCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IVCKRFGEAIGRAAQGFEVKELIIAPKEKVDAYELMEFIKGVKIGQHSRYEIQKRAYPFE
HHHHHHHHHHHHHHCCCCHHHHEECCHHHCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEE
IKLVPVTVQDLYQVVRDKRRDNRLLIITDPKGDELSKIKDKLAYDLRKKREIIVFCGSRE
EEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
GIPRGLFRFADYIVDLAPHMTFATEHAIPAALIALWGVYSGEDLENSSKEEKTESNSNGE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKFIIKTQKGFENIVVNNLKEIVDDFNYIVSPDGYQGIVIVESDEDIEDKILQIPEVERV
CCEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCHHHHHHCCCHHHHH
LKVYFETETDFDKIVNLAEKIKDYIKEDETFAVETKKRGKHDFSSTDINIVLGAKIKDLT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCEEEEECCEEHHCC
NASVDLNNPDKVVHVEVFKNKTYVSITPGEKFKKYTKEKRNARELFKKVVIVQMPYLGEK
CCEEECCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IVCKRFGEAIGRAAQGFEVKELIIAPKEKVDAYELMEFIKGVKIGQHSRYEIQKRAYPFE
HHHHHHHHHHHHHHCCCCHHHHEECCHHHCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEE
IKLVPVTVQDLYQVVRDKRRDNRLLIITDPKGDELSKIKDKLAYDLRKKREIIVFCGSRE
EEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
GIPRGLFRFADYIVDLAPHMTFATEHAIPAALIALWGVYSGEDLENSSKEEKTESNSNGE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087