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The map label for this gene is cbiT

Identifier: 15668567

GI number: 15668567

Start: 355153

End: 355704

Strand: Reverse

Name: cbiT

Synonym: MJ0391

Alternate gene names: 15668567

Gene position: 355704-355153 (Counterclockwise)

Preceding gene: 15668569

Following gene: 15668566

Centisome position: 21.36

GC content: 28.8

Gene sequence:

>552_bases
GTGAAATATATGATTCCAGATGAAGAATTTATAAGAAGAGAAGGAGTTCCAATAACAAAAGAAGAAATTAGGGCTGTGAG
TATTGGGAAATTAAACTTAAATAAAGATGACGTTGTTGTTGATGTTGGTTGTGGAAGTGGAGGAATGACAGTTGAGATAG
CAAAGAGATGCAAGTTTGTTTATGCTATAGATTATTTAGATGGCGCTATTGAAGTAACTAAACAAAATTTAGCCAAATTT
AATATTAAAAATTGCCAAATAATAAAGGGAAGGGCAGAAGATGTTTTAGATAAATTAGAATTTAATAAAGCTTTTATAGG
TGGGACAAAAAATATTGAAAAGATAATTGAAATTTTGGATAAAAAGAAAATAAATCACATTGTTGCTAACACAATTGTTT
TAGAAAATGCTGCTAAAATAATAAATGAATTTGAGAGTAGAGGTTACAATGTTGATGCCGTTAATGTTTTTATTTCTTAT
GCTAAAAAAATCCCTTCTGGACACATGTTTTTGGCAAAGAATCCAATAACTATAATAAAAGCAGTTAGGTAG

Upstream 100 bases:

>100_bases
ATCTTATTGAGTAATTCATACCCCTCCCCCTATTTTTATTTTAATTACATTTTTAATCCTATTGCTATATATTACTATTT
CATAACATATTTATGATTCG

Downstream 100 bases:

>100_bases
ATAATCATGGAAGAGAAAATAATCCTATCAATCCAAAACCCAGAAGATGTTTTAATTTCTTATGTTGATATTTACTTAGG
AGATAAAAATGTTTCATTGG

Product: cobalamin biosynthesis precorrin-8W decarboxylase CbiT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MKYMIPDEEFIRREGVPITKEEIRAVSIGKLNLNKDDVVVDVGCGSGGMTVEIAKRCKFVYAIDYLDGAIEVTKQNLAKF
NIKNCQIIKGRAEDVLDKLEFNKAFIGGTKNIEKIIEILDKKKINHIVANTIVLENAAKIINEFESRGYNVDAVNVFISY
AKKIPSGHMFLAKNPITIIKAVR

Sequences:

>Translated_183_residues
MKYMIPDEEFIRREGVPITKEEIRAVSIGKLNLNKDDVVVDVGCGSGGMTVEIAKRCKFVYAIDYLDGAIEVTKQNLAKF
NIKNCQIIKGRAEDVLDKLEFNKAFIGGTKNIEKIIEILDKKKINHIVANTIVLENAAKIINEFESRGYNVDAVNVFISY
AKKIPSGHMFLAKNPITIIKAVR
>Mature_183_residues
MKYMIPDEEFIRREGVPITKEEIRAVSIGKLNLNKDDVVVDVGCGSGGMTVEIAKRCKFVYAIDYLDGAIEVTKQNLAKF
NIKNCQIIKGRAEDVLDKLEFNKAFIGGTKNIEKIIEILDKKKINHIVANTIVLENAAKIINEFESRGYNVDAVNVFISY
AKKIPSGHMFLAKNPITIIKAVR

Specific function: Probably catalyzes the methylation of either C-15 or C-5 in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of C-15 would probably be followed by a spontaneous decarboxylation of C-12

COG id: COG2242

COG function: function code H; Precorrin-6B methylase 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Archaeal-type CbiT family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIT_METJA (Q57836)

Other databases:

- EMBL:   L77117
- PIR:   G64348
- RefSeq:   NP_247365.1
- PDB:   2YXD
- PDBsum:   2YXD
- ProteinModelPortal:   Q57836
- SMR:   Q57836
- GeneID:   1451248
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0391
- NMPDR:   fig|243232.1.peg.402
- TIGR:   MJ0391
- HOGENOM:   HBG553505
- OMA:   IRAVSIG
- ProtClustDB:   CLSK876165
- BioCyc:   MJAN243232:MJ_0391-MONOMER
- HAMAP:   MF_00786
- InterPro:   IPR014008
- InterPro:   IPR007848
- TIGRFAMs:   TIGR02469

Pfam domain/function: PF05175 MTS

EC number: 1.-.-.-

Molecular weight: Translated: 20502; Mature: 20502

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: BINDING 19-19 BINDING 64-64 BINDING 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYMIPDEEFIRREGVPITKEEIRAVSIGKLNLNKDDVVVDVGCGSGGMTVEIAKRCKFV
CCCCCCCHHHHHHCCCCCCHHHHEEEEEEEEECCCCCEEEEECCCCCCCEEEEHHHCEEE
YAIDYLDGAIEVTKQNLAKFNIKNCQIIKGRAEDVLDKLEFNKAFIGGTKNIEKIIEILD
EEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCHHEECCCCCHHHHHHHHH
KKKINHIVANTIVLENAAKIINEFESRGYNVDAVNVFISYAKKIPSGHMFLAKNPITIIK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEE
AVR
ECC
>Mature Secondary Structure
MKYMIPDEEFIRREGVPITKEEIRAVSIGKLNLNKDDVVVDVGCGSGGMTVEIAKRCKFV
CCCCCCCHHHHHHCCCCCCHHHHEEEEEEEEECCCCCEEEEECCCCCCCEEEEHHHCEEE
YAIDYLDGAIEVTKQNLAKFNIKNCQIIKGRAEDVLDKLEFNKAFIGGTKNIEKIIEILD
EEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCHHEECCCCCHHHHHHHHH
KKKINHIVANTIVLENAAKIINEFESRGYNVDAVNVFISYAKKIPSGHMFLAKNPITIIK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEE
AVR
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087