Click here to switch to the map view.

The map label for this gene is yeiE [C]

Identifier: 15668475

GI number: 15668475

Start: 283277

End: 284167

Strand: Direct

Name: yeiE [C]

Synonym: MJ0300

Alternate gene names: 15668475

Gene position: 283277-284167 (Clockwise)

Preceding gene: 15668473

Following gene: 15668480

Centisome position: 17.01

GC content: 33.45

Gene sequence:

>891_bases
ATGGATCCAAAAATAAGTTATTTTCAAACATTTATAGTTGCAAGTAAAACAAAAAGTTTTTCTAAGGCAGCAAAAAGATT
GGGAATTACTCAAGGAACCGTCAGTAATCACATATCAGCACTTGAGAAATACTTCGATGCCCAACTCTTTTTGAGAACTC
CTGAGGGAGTTGATTTAACTCCTGAAGGAAAGATATTTTATGAAAGGGCTGAAAAGATTTTAGACTTGTTAAATGAGGCA
AAATTGTTGATGAGAGCCATACATGAAAATCCAGAGGGAATTATCAGAATTTATGCCTCTACAACCCCTGGAGAGCATAT
ATTACCATCAATTATTAAGGAGTATAAAAGCTCATACAAAAATGTTGATTTTGAGATTACAATAACTGACTCTGAGAGAT
GTTTTAAAGCTTTAGATGAAGGATTGGCAGATATAGCAGCAGTTGGTTATCTAAAAAATAAGAATTATGAATATACAATT
ATAGGTAAAGATAGATTGGTTTTAATTGTCCCACCAAATCATCCACTTGCAGAGAAAGGCACTGCTAAGCTTGAGGATAT
ACTTAAAGAGGATTACATTGATAGAGAGGAGGGTTCTGGAACAAGAGAGGCATTTATAAAGGCTTTAAATGATAAGGGAT
ATTCAATAATGGATTTAAACGTTGTAATGAGGTTGGGTAGTCATTCAGCAGTTATAACTGCAGTTTCTGAAGGTTATGGA
GTTAGCGTAGTTTCAGAAATCCCTGCTAAAAAGGCAGAAGATGCTGGATTAATTAAGATAGTTCCAGTTGTAGATTTGGA
TGTTGTTAGATATTTATATTTAGTTAAAAGTAGAAGACCAAAAAATCCAAGTGCTGTTAAATCATTCTGGGAATTTGTTA
CAAAGGTTTAA

Upstream 100 bases:

>100_bases
TTACTATTCCATTGGTACTTAAAATTCACTACACATTAACTTTATATATAGGAATTATTAGTTATTCTAATAGGGATTAA
GTAACATAGGTGATAATAAT

Downstream 100 bases:

>100_bases
ATTAGTGTCTTTTATATTACAAAATGTTTTGAAAGACACTATCGTTTTAAAATTTTATAATCTTTCCAGCATGTAACCTC
TCAAAATCTTCTTTGAACTC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDPKISYFQTFIVASKTKSFSKAAKRLGITQGTVSNHISALEKYFDAQLFLRTPEGVDLTPEGKIFYERAEKILDLLNEA
KLLMRAIHENPEGIIRIYASTTPGEHILPSIIKEYKSSYKNVDFEITITDSERCFKALDEGLADIAAVGYLKNKNYEYTI
IGKDRLVLIVPPNHPLAEKGTAKLEDILKEDYIDREEGSGTREAFIKALNDKGYSIMDLNVVMRLGSHSAVITAVSEGYG
VSVVSEIPAKKAEDAGLIKIVPVVDLDVVRYLYLVKSRRPKNPSAVKSFWEFVTKV

Sequences:

>Translated_296_residues
MDPKISYFQTFIVASKTKSFSKAAKRLGITQGTVSNHISALEKYFDAQLFLRTPEGVDLTPEGKIFYERAEKILDLLNEA
KLLMRAIHENPEGIIRIYASTTPGEHILPSIIKEYKSSYKNVDFEITITDSERCFKALDEGLADIAAVGYLKNKNYEYTI
IGKDRLVLIVPPNHPLAEKGTAKLEDILKEDYIDREEGSGTREAFIKALNDKGYSIMDLNVVMRLGSHSAVITAVSEGYG
VSVVSEIPAKKAEDAGLIKIVPVVDLDVVRYLYLVKSRRPKNPSAVKSFWEFVTKV
>Mature_296_residues
MDPKISYFQTFIVASKTKSFSKAAKRLGITQGTVSNHISALEKYFDAQLFLRTPEGVDLTPEGKIFYERAEKILDLLNEA
KLLMRAIHENPEGIIRIYASTTPGEHILPSIIKEYKSSYKNVDFEITITDSERCFKALDEGLADIAAVGYLKNKNYEYTI
IGKDRLVLIVPPNHPLAEKGTAKLEDILKEDYIDREEGSGTREAFIKALNDKGYSIMDLNVVMRLGSHSAVITAVSEGYG
VSVVSEIPAKKAEDAGLIKIVPVVDLDVVRYLYLVKSRRPKNPSAVKSFWEFVTKV

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788481, Length=297, Percent_Identity=28.6195286195286, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=25.5892255892256, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI145693105, Length=279, Percent_Identity=23.6559139784946, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081904, Length=223, Percent_Identity=26.457399103139, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=24.1610738255034, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693193, Length=264, Percent_Identity=27.6515151515151, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789173, Length=283, Percent_Identity=25.7950530035336, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=177, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=250, Percent_Identity=24.4, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=22.9965156794425, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787601, Length=175, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787128, Length=173, Percent_Identity=28.3236994219653, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=23.8636363636364, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=121, Percent_Identity=28.9256198347107, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): Y300_METJA (Q57748)

Other databases:

- EMBL:   L77117
- PIR:   E64337
- RefSeq:   NP_247273.1
- ProteinModelPortal:   Q57748
- GeneID:   1451155
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0300
- NMPDR:   fig|243232.1.peg.310
- TIGR:   MJ0300
- HOGENOM:   HBG685425
- OMA:   GTVSNHI
- ProtClustDB:   CLSK876149
- BioCyc:   MJAN243232:MJ_0300-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33112; Mature: 33112

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKISYFQTFIVASKTKSFSKAAKRLGITQGTVSNHISALEKYFDAQLFLRTPEGVDLT
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
PEGKIFYERAEKILDLLNEAKLLMRAIHENPEGIIRIYASTTPGEHILPSIIKEYKSSYK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHC
NVDFEITITDSERCFKALDEGLADIAAVGYLKNKNYEYTIIGKDRLVLIVPPNHPLAEKG
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCCC
TAKLEDILKEDYIDREEGSGTREAFIKALNDKGYSIMDLNVVMRLGSHSAVITAVSEGYG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHCCCCCEEEEEECCCCC
VSVVSEIPAKKAEDAGLIKIVPVVDLDVVRYLYLVKSRRPKNPSAVKSFWEFVTKV
CHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDPKISYFQTFIVASKTKSFSKAAKRLGITQGTVSNHISALEKYFDAQLFLRTPEGVDLT
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
PEGKIFYERAEKILDLLNEAKLLMRAIHENPEGIIRIYASTTPGEHILPSIIKEYKSSYK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHC
NVDFEITITDSERCFKALDEGLADIAAVGYLKNKNYEYTIIGKDRLVLIVPPNHPLAEKG
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCCC
TAKLEDILKEDYIDREEGSGTREAFIKALNDKGYSIMDLNVVMRLGSHSAVITAVSEGYG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHCCCCCEEEEEECCCCC
VSVVSEIPAKKAEDAGLIKIVPVVDLDVVRYLYLVKSRRPKNPSAVKSFWEFVTKV
CHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087