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The map label for this gene is ilvB

Identifier: 15668452

GI number: 15668452

Start: 262848

End: 264623

Strand: Reverse

Name: ilvB

Synonym: MJ0277

Alternate gene names: 15668452

Gene position: 264623-262848 (Counterclockwise)

Preceding gene: 15668453

Following gene: 15668451

Centisome position: 15.89

GC content: 37.95

Gene sequence:

>1776_bases
ATGAAAGGTGCTGAAGCAATCATAAAAGCATTGGAAGCGGAGGGAGTTAAGATTATATTTGGTTATCCAGGAGGAGCTAT
GCTTCCTTTTTATGATGCGTTGTATGATAGCGATTTAGTTCATATATTAACAAGGCATGAACAGGCAGCAGCACATGCAG
CAGATGGATTTGCGAGAGCAAGTGGAGAGGCTGGGGTTTGCGTCTCTACCTCTGGCCCTGGAGCTACAAACTTAGTTACT
GGGATAGCAACCGCTTATGCAGATTCTTCTCCAGTTATTGCTTTAACAGGGCAAGTCCCAACAAAACTTATTGGAAACGA
TGCATTTCAGGAGATTGACGCTCTTGGATTATTCATGCCAATAACAAAACACAATTTCCAAATAAAAAAACCAGAAGAGA
TTCCAGAGACGTTTAGAGCCGCTTTTGAAATTGCCACAACTGGAAGACCAGGACCGGTTCATATAGACCTCCCAAAGGAT
GTGCAAGATGGAGAAATAGATATTGAAAAATACCCAATTCCTGCAAAGGTTGATTTGCCAGGTTATAAACCAAAAACTGT
AGGGCATCCTCTACAGATAAAGAAAGCTGCTAAATTGATAGCTGAATCTGAGAGACCTGTAATCTTAGCTGGTGGAGGAG
TTATAATTAGTGGAGCTTCAGAAGAGTTATTGAGATTAGCTGAGTTTGTTAAAATTCCAGTATGCACAACCTTAATGGGT
AAGGGTTGTTTCCCAGAAGACCATCCTTTAGCTTTAGGAATGGTTGGAATGCATGGAACTAAAGCTGCAAATTACGCAGT
TACGGAGTGTGATGTTCTCATAGCTATTGGATGTAGATTTTCAGATAGGGTTACTGGGGATATCAGATACTTTGCTCCAG
AGGCAAAGATTATTCATATAGATATAGACCCAGCTGAGATAGGAAAAAATGTTAGAGCTGATATTCCAATAGTTGGAGAT
GCAAAAAATGTTTTGAGAGATTTGTTAGCTGCATTAATAGCATTAGAAATTAAAGACAAAGAAACATGGCTTGAAAGAAT
TTATGAATTAAAAAAATTATCTATCCCAATGATGGACTTTGATGATAAGCCAATAAAGCCACAAAGGTTTGTTAAGGATT
TAATGGAAGTTTTGAATGAGATTGACTCAAAATTAAAAAACACAATTATAACAACAGATGTTGGACAAAATCAGATGTGG
ATGGCACACTTCTTTAAAACAAAGATGCCAAGAAGCTTTTTAGCTTCTGGTGGTTTAGGAACTATGGGTTTTGGTTTCCC
TGCTGCAATTGGGGCAAAGGTAGCTAAACCTTATGCTAATGTTATCTCTATTACTGGAGATGGAGGATTTTTGATGAACT
CTCAGGAGTTGGCAACAATTAGCGAATATGATATTCCTGTTGTTATCTGTATTTTTGACAACAGAACTTTGGGAATGGTC
TATCAATGGCAAAACCTATACTATGGGCAGAGGCAGAGTGAAGTTCATTTGGGAGAGAGTCCTGACTTTGTTAAATTAGC
TGAAAGTTATGGAGTTAAAGCTGATAGAATAATAAGCCCAGATGAAATTAAAGAGAAGTTGAAAGAAGCAATATTAAGTA
ATGAGCCATACCTCTTAGATATTGTTATAGACCCTGCTGAAGCTCTGCCAATGGTTCCTCCAGGTGGGAGATTAACCAAT
ATTGTCCAGCCAATTAGGGTAGAACCAAAAATAAAAAAACCACAGTTCGATGAAATTAAGAAAATAAGAGATATGGCAGC
AGTTAAAGAGTTTTAG

Upstream 100 bases:

>100_bases
CTTTTTAAAGTTCTTCAAATGCTAAATCCTTAGTATAAAAATTTTTATATATGATTTCAATTTTATCATTACTTTACCCT
TAACATTTTTTGGTGATTGG

Downstream 100 bases:

>100_bases
ATAAATTAGCCCATGCTTCTATTTTTTTAAATTGTTATTTTCTTCTCTATTATATTATAGTCGTTAAATATTAACACAAG
GTTATATTATATAAAAGTAG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKGAEAIIKALEAEGVKIIFGYPGGAMLPFYDALYDSDLVHILTRHEQAAAHAADGFARASGEAGVCVSTSGPGATNLVT
GIATAYADSSPVIALTGQVPTKLIGNDAFQEIDALGLFMPITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPKD
VQDGEIDIEKYPIPAKVDLPGYKPKTVGHPLQIKKAAKLIAESERPVILAGGGVIISGASEELLRLAEFVKIPVCTTLMG
KGCFPEDHPLALGMVGMHGTKAANYAVTECDVLIAIGCRFSDRVTGDIRYFAPEAKIIHIDIDPAEIGKNVRADIPIVGD
AKNVLRDLLAALIALEIKDKETWLERIYELKKLSIPMMDFDDKPIKPQRFVKDLMEVLNEIDSKLKNTIITTDVGQNQMW
MAHFFKTKMPRSFLASGGLGTMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQELATISEYDIPVVICIFDNRTLGMV
YQWQNLYYGQRQSEVHLGESPDFVKLAESYGVKADRIISPDEIKEKLKEAILSNEPYLLDIVIDPAEALPMVPPGGRLTN
IVQPIRVEPKIKKPQFDEIKKIRDMAAVKEF

Sequences:

>Translated_591_residues
MKGAEAIIKALEAEGVKIIFGYPGGAMLPFYDALYDSDLVHILTRHEQAAAHAADGFARASGEAGVCVSTSGPGATNLVT
GIATAYADSSPVIALTGQVPTKLIGNDAFQEIDALGLFMPITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPKD
VQDGEIDIEKYPIPAKVDLPGYKPKTVGHPLQIKKAAKLIAESERPVILAGGGVIISGASEELLRLAEFVKIPVCTTLMG
KGCFPEDHPLALGMVGMHGTKAANYAVTECDVLIAIGCRFSDRVTGDIRYFAPEAKIIHIDIDPAEIGKNVRADIPIVGD
AKNVLRDLLAALIALEIKDKETWLERIYELKKLSIPMMDFDDKPIKPQRFVKDLMEVLNEIDSKLKNTIITTDVGQNQMW
MAHFFKTKMPRSFLASGGLGTMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQELATISEYDIPVVICIFDNRTLGMV
YQWQNLYYGQRQSEVHLGESPDFVKLAESYGVKADRIISPDEIKEKLKEAILSNEPYLLDIVIDPAEALPMVPPGGRLTN
IVQPIRVEPKIKKPQFDEIKKIRDMAAVKEF
>Mature_591_residues
MKGAEAIIKALEAEGVKIIFGYPGGAMLPFYDALYDSDLVHILTRHEQAAAHAADGFARASGEAGVCVSTSGPGATNLVT
GIATAYADSSPVIALTGQVPTKLIGNDAFQEIDALGLFMPITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPKD
VQDGEIDIEKYPIPAKVDLPGYKPKTVGHPLQIKKAAKLIAESERPVILAGGGVIISGASEELLRLAEFVKIPVCTTLMG
KGCFPEDHPLALGMVGMHGTKAANYAVTECDVLIAIGCRFSDRVTGDIRYFAPEAKIIHIDIDPAEIGKNVRADIPIVGD
AKNVLRDLLAALIALEIKDKETWLERIYELKKLSIPMMDFDDKPIKPQRFVKDLMEVLNEIDSKLKNTIITTDVGQNQMW
MAHFFKTKMPRSFLASGGLGTMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQELATISEYDIPVVICIFDNRTLGMV
YQWQNLYYGQRQSEVHLGESPDFVKLAESYGVKADRIISPDEIKEKLKEAILSNEPYLLDIVIDPAEALPMVPPGGRLTN
IVQPIRVEPKIKKPQFDEIKKIRDMAAVKEF

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=25.2650176678445, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI21361361, Length=509, Percent_Identity=27.8978388998035, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=44.8943661971831, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=43.2624113475177, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786717, Length=584, Percent_Identity=33.7328767123288, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=29.9645390070922, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=27.2890484739677, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17531299, Length=483, Percent_Identity=28.1573498964803, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=483, Percent_Identity=28.1573498964803, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=517, Percent_Identity=26.4990328820116, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323755, Length=597, Percent_Identity=42.3785594639866, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=24.1563055062167, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323163, Length=520, Percent_Identity=25.9615384615385, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=517, Percent_Identity=24.5647969052224, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=515, Percent_Identity=25.4368932038835, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=25.2189141856392, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVB_METJA (Q57725)

Other databases:

- EMBL:   L77117
- PIR:   F64334
- RefSeq:   NP_247250.1
- ProteinModelPortal:   Q57725
- SMR:   Q57725
- GeneID:   1451132
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0277
- NMPDR:   fig|243232.1.peg.287
- TIGR:   MJ0277
- HOGENOM:   HBG323037
- OMA:   PVVICIF
- ProtClustDB:   PRK06276
- BioCyc:   MJAN243232:MJ_0277-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 64494; Mature: 64494

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 47-47 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGAEAIIKALEAEGVKIIFGYPGGAMLPFYDALYDSDLVHILTRHEQAAAHAADGFARA
CCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHC
SGEAGVCVSTSGPGATNLVTGIATAYADSSPVIALTGQVPTKLIGNDAFQEIDALGLFMP
CCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCHHHHHHHHHHEEEE
ITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPKDVQDGEIDIEKYPIPAKVDLP
ECCCCEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCEEEEEECCCCEEECCC
GYKPKTVGHPLQIKKAAKLIAESERPVILAGGGVIISGASEELLRLAEFVKIPVCTTLMG
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHHHHHHHCCHHHHHHC
KGCFPEDHPLALGMVGMHGTKAANYAVTECDVLIAIGCRFSDRVTGDIRYFAPEAKIIHI
CCCCCCCCCEEEEEECCCCCCCCCEEEEECEEEEEECCCCCCCCCCCEEEECCCCEEEEE
DIDPAEIGKNVRADIPIVGDAKNVLRDLLAALIALEIKDKETWLERIYELKKLSIPMMDF
ECCHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCC
DDKPIKPQRFVKDLMEVLNEIDSKLKNTIITTDVGQNQMWMAHFFKTKMPRSFLASGGLG
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCHHHHHCCCCC
TMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQELATISEYDIPVVICIFDNRTLGMV
CCCCCCHHHHHHHHHCCCCCEEEEECCCCEEECCCHHCEEECCCCCEEEEEECCCEEEEE
YQWQNLYYGQRQSEVHLGESPDFVKLAESYGVKADRIISPDEIKEKLKEAILSNEPYLLD
EEECHHCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
IVIDPAEALPMVPPGGRLTNIVQPIRVEPKIKKPQFDEIKKIRDMAAVKEF
EEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGAEAIIKALEAEGVKIIFGYPGGAMLPFYDALYDSDLVHILTRHEQAAAHAADGFARA
CCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHC
SGEAGVCVSTSGPGATNLVTGIATAYADSSPVIALTGQVPTKLIGNDAFQEIDALGLFMP
CCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCHHHHHHHHHHEEEE
ITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPKDVQDGEIDIEKYPIPAKVDLP
ECCCCEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCEEEEEECCCCEEECCC
GYKPKTVGHPLQIKKAAKLIAESERPVILAGGGVIISGASEELLRLAEFVKIPVCTTLMG
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHHHHHHHCCHHHHHHC
KGCFPEDHPLALGMVGMHGTKAANYAVTECDVLIAIGCRFSDRVTGDIRYFAPEAKIIHI
CCCCCCCCCEEEEEECCCCCCCCCEEEEECEEEEEECCCCCCCCCCCEEEECCCCEEEEE
DIDPAEIGKNVRADIPIVGDAKNVLRDLLAALIALEIKDKETWLERIYELKKLSIPMMDF
ECCHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCC
DDKPIKPQRFVKDLMEVLNEIDSKLKNTIITTDVGQNQMWMAHFFKTKMPRSFLASGGLG
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCHHHHHCCCCC
TMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQELATISEYDIPVVICIFDNRTLGMV
CCCCCCHHHHHHHHHCCCCCEEEEECCCCEEECCCHHCEEECCCCCEEEEEECCCEEEEE
YQWQNLYYGQRQSEVHLGESPDFVKLAESYGVKADRIISPDEIKEKLKEAILSNEPYLLD
EEECHHCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
IVIDPAEALPMVPPGGRLTNIVQPIRVEPKIKKPQFDEIKKIRDMAAVKEF
EEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087