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The map label for this gene is eno [H]

Identifier: 15668407

GI number: 15668407

Start: 222923

End: 224206

Strand: Reverse

Name: eno [H]

Synonym: MJ0232

Alternate gene names: 15668407

Gene position: 224206-222923 (Counterclockwise)

Preceding gene: 15668415

Following gene: 15668406

Centisome position: 13.47

GC content: 36.14

Gene sequence:

>1284_bases
TTGTTATATAATATGGATGAGAGATTTGAAATTAAAGATATTGTTGCAAGAGAAGTAATTGACTCAAGAGGAAACCCAAC
AGTTGAAGTGGAAGTTATAACAAAAGGGAATGGTTACGGTTCAGCAATTGTTCCAAGTGGTGCATCAACTGGAACACATG
AGGCATTAGAGTTGAGGGATAAAGAAAAGAGATTTGGTGGAAAAGGAGTTTTAATGGCTGTTGAAAATGTAAATTCAATA
ATTAGACCAGAGATTTTAGGTTATGATGCAAGAATGCAGAGAGAAATAGATACAATAATGATAGAATTAGATGGTACTCC
AAATAAATCAAGATTGGGAGCTAATGCCATATTGGCTGTTTCTTTAGCTGTAGCAAAGGCAGCAGCAGCAACAGCAAAAA
TCCCTCTCTATAAATACTTGGGGGGATTTAACTCCTATGTCATGCCAGTTCCAATGATGAACGTTATAAATGGAGGAAAA
CACGCTGGGAATGATTTAGATTTGCAAGAGTTCATGATAATGCCAGTTGGAGCTACATCAATTTCTGAAGCTGTAAGGAT
GGGTTCAGAAGTTTATCATGTCTTAAAAAATGTCATCTTAGAAAAATATGGAAAAAATGCTGTAAATGTTGGAGATGAGG
GAGGTTTTGCTCCACCATTAAAAACATCAAGGGAGGCTTTAGATTTATTAACTGAGAGTGTTAAAAAGGCTGGGTATGAG
GATGAGGTTGTCTTTGCATTAGATGCTGCTGCCTCAGAGTTTTATAAAGATGGATATTATTACGTTGAAGGTAAAAAATT
AACAAGAGAGGAGCTTTTAGATTACTATAAAGCATTAGTTGATGAATATCCAATAGTCTCAATTGAAGACCCATTCCATG
AGGAAGATTTTGAAGGCTTTGCAATGATAACTAAAGAATTAGATATACAGATAGTTGGAGATGACTTGTTTGTTACAAAT
GTTGAAAGGCTTAGAAAAGGTATTGAGATGAAGGCTGCTAACGCTCTGCTTTTGAAAGTCAATCAGATTGGAACTTTAAG
TGAGGCAGTTGATGCTGCTCAATTGGCATTTAGAAATGGTTATGGTGTAGTTGTTTCACATAGAAGTGGAGAGACTGAGG
ATACAACAATAGCTGATTTGTCAGTTGCTTTGAACTCTGGACAAATAAAGACTGGAGCTCCAGCAAGAGGGGAGAGAACA
GCTAAATACAATCAGTTGATAAGAATTGAGCAAGAGTTAGGATTAAGCAAATATGCTGGGAGAAACTTTAGATGTCCATT
TTAA

Upstream 100 bases:

>100_bases
TTCGTTAAATAACTCTCAATAGTTCCCATATAACTTCACCTCCAAGTTTATTATAAAAATTCATAATTAAAGTATTTAAA
AATATCTATTAGGTGATAAT

Downstream 100 bases:

>100_bases
ATTTTTCTAATTTTTTAATACCTAAGTTTTAAGGTTTTCATCCAATCTTTCCTAAAAGTTTCTTTCAAAATGTTTTACAA
GAAACTATATTTCAACTTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLYNMDERFEIKDIVAREVIDSRGNPTVEVEVITKGNGYGSAIVPSGASTGTHEALELRDKEKRFGGKGVLMAVENVNSI
IRPEILGYDARMQREIDTIMIELDGTPNKSRLGANAILAVSLAVAKAAAATAKIPLYKYLGGFNSYVMPVPMMNVINGGK
HAGNDLDLQEFMIMPVGATSISEAVRMGSEVYHVLKNVILEKYGKNAVNVGDEGGFAPPLKTSREALDLLTESVKKAGYE
DEVVFALDAAASEFYKDGYYYVEGKKLTREELLDYYKALVDEYPIVSIEDPFHEEDFEGFAMITKELDIQIVGDDLFVTN
VERLRKGIEMKAANALLLKVNQIGTLSEAVDAAQLAFRNGYGVVVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERT
AKYNQLIRIEQELGLSKYAGRNFRCPF

Sequences:

>Translated_427_residues
MLYNMDERFEIKDIVAREVIDSRGNPTVEVEVITKGNGYGSAIVPSGASTGTHEALELRDKEKRFGGKGVLMAVENVNSI
IRPEILGYDARMQREIDTIMIELDGTPNKSRLGANAILAVSLAVAKAAAATAKIPLYKYLGGFNSYVMPVPMMNVINGGK
HAGNDLDLQEFMIMPVGATSISEAVRMGSEVYHVLKNVILEKYGKNAVNVGDEGGFAPPLKTSREALDLLTESVKKAGYE
DEVVFALDAAASEFYKDGYYYVEGKKLTREELLDYYKALVDEYPIVSIEDPFHEEDFEGFAMITKELDIQIVGDDLFVTN
VERLRKGIEMKAANALLLKVNQIGTLSEAVDAAQLAFRNGYGVVVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERT
AKYNQLIRIEQELGLSKYAGRNFRCPF
>Mature_427_residues
MLYNMDERFEIKDIVAREVIDSRGNPTVEVEVITKGNGYGSAIVPSGASTGTHEALELRDKEKRFGGKGVLMAVENVNSI
IRPEILGYDARMQREIDTIMIELDGTPNKSRLGANAILAVSLAVAKAAAATAKIPLYKYLGGFNSYVMPVPMMNVINGGK
HAGNDLDLQEFMIMPVGATSISEAVRMGSEVYHVLKNVILEKYGKNAVNVGDEGGFAPPLKTSREALDLLTESVKKAGYE
DEVVFALDAAASEFYKDGYYYVEGKKLTREELLDYYKALVDEYPIVSIEDPFHEEDFEGFAMITKELDIQIVGDDLFVTN
VERLRKGIEMKAANALLLKVNQIGTLSEAVDAAQLAFRNGYGVVVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERT
AKYNQLIRIEQELGLSKYAGRNFRCPF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=56.7441860465116, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=56.1484918793503, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=56.2790697674419, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=56.2790697674419, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=51.4018691588785, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=28.486646884273, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=28.486646884273, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=28.486646884273, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=56.9411764705882, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=55.632183908046, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=55.9164733178654, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=53.0927835051546, Blast_Score=211, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=56.3805104408353, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=54.0415704387991, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=54.0415704387991, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=54.0415704387991, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=55.9164733178654, Blast_Score=429, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=55.6844547563805, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=55.6844547563805, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=55.6844547563805, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=55.6844547563805, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=55.6844547563805, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=55.6844547563805, Blast_Score=450, Evalue=1e-127,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46820; Mature: 46820

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYNMDERFEIKDIVAREVIDSRGNPTVEVEVITKGNGYGSAIVPSGASTGTHEALELRD
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHH
KEKRFGGKGVLMAVENVNSIIRPEILGYDARMQREIDTIMIELDGTPNKSRLGANAILAV
HHHHCCCCEEEEEEHHHHHHHCHHHCCCCHHHHHCEEEEEEEECCCCCHHHCCCHHHHHH
SLAVAKAAAATAKIPLYKYLGGFNSYVMPVPMMNVINGGKHAGNDLDLQEFMIMPVGATS
HHHHHHHHHHHHCCHHHHHHCCCCCEECCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHH
ISEAVRMGSEVYHVLKNVILEKYGKNAVNVGDEGGFAPPLKTSREALDLLTESVKKAGYE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DEVVFALDAAASEFYKDGYYYVEGKKLTREELLDYYKALVDEYPIVSIEDPFHEEDFEGF
CCEEEEEEHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
AMITKELDIQIVGDDLFVTNVERLRKGIEMKAANALLLKVNQIGTLSEAVDAAQLAFRNG
EEEEEECEEEEECCCEEEECHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHHHHHCCC
YGVVVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERTAKYNQLIRIEQELGLSKYAG
CEEEEECCCCCCCCCEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHCC
RNFRCPF
CCCCCCC
>Mature Secondary Structure
MLYNMDERFEIKDIVAREVIDSRGNPTVEVEVITKGNGYGSAIVPSGASTGTHEALELRD
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHH
KEKRFGGKGVLMAVENVNSIIRPEILGYDARMQREIDTIMIELDGTPNKSRLGANAILAV
HHHHCCCCEEEEEEHHHHHHHCHHHCCCCHHHHHCEEEEEEEECCCCCHHHCCCHHHHHH
SLAVAKAAAATAKIPLYKYLGGFNSYVMPVPMMNVINGGKHAGNDLDLQEFMIMPVGATS
HHHHHHHHHHHHCCHHHHHHCCCCCEECCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHH
ISEAVRMGSEVYHVLKNVILEKYGKNAVNVGDEGGFAPPLKTSREALDLLTESVKKAGYE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DEVVFALDAAASEFYKDGYYYVEGKKLTREELLDYYKALVDEYPIVSIEDPFHEEDFEGF
CCEEEEEEHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
AMITKELDIQIVGDDLFVTNVERLRKGIEMKAANALLLKVNQIGTLSEAVDAAQLAFRNG
EEEEEECEEEEECCCEEEECHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHHHHHCCC
YGVVVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERTAKYNQLIRIEQELGLSKYAG
CEEEEECCCCCCCCCEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHCC
RNFRCPF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]