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The map label for this gene is rpoD

Identifier: 15668364

GI number: 15668364

Start: 188638

End: 189213

Strand: Direct

Name: rpoD

Synonym: MJ0192

Alternate gene names: 15668364

Gene position: 188638-189213 (Clockwise)

Preceding gene: 15668363

Following gene: 15668365

Centisome position: 11.33

GC content: 30.73

Gene sequence:

>576_bases
TTGATTACAATCAAAGAAAAGAGAAAGACAAGAATTGGGGAGGAATTTATTTTTTCTTTAAAAGCCCCAATTTCATTTTC
TAATGCTATTAGGAGAATAATGATTTCTGAAGTTCCAACCTTTGCTATTGAAGATGTTTATATATATGAGAACTCATCAT
CAATGGATGATGAGATTTTAGCACATAGATTGGGTTTAATTCCAATTAAAGGAAAACCATTATTAGAGAATGAAGTTATA
ACATTCACCTTAGAAAAAGAGGGGCCTTGCACAGTTTATTCATCAGATTTAAAATCTGAAAATGGAGAAGTTGCTTTTAA
AAACATTCCAATTGTTAAATTAGGAAAAGGGCAAAGAATACAGATTGAATGTGAAGCAATTCCTGGCATTGGAAAGGTTC
ATGCAAAATGGCAACCATGCAATGCTGTCTATAAACAAATTGCTGATGATGAAGTAGAATTTTTCGTTGAAACATTTGGA
CAAATGGAAGCTGAAGAAATCTTAGAAGAAGCTGTTAAAATACTAAAAAATAAAGCTGAAAGTTTCTTACAACAGTTAGA
AATGATTGAACAATAA

Upstream 100 bases:

>100_bases
CAAGAGCTGGATTAAGAATTGGAAGAATTGAAGATGTTACACCAGTTCCACATGATGGAACAACACCTAAGAAGAGGTTC
AAAAAGTAAGGTGATAATCT

Downstream 100 bases:

>100_bases
AAAACCATTTTCATTCTAATTATTTGTCAAACCTTATAATAATTATAAAAGGTTTGAAGGAAAACACGGTGATATATATG
GCAAAGAAAATAACAGCTAC

Product: DNA-directed RNA polymerase subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MITIKEKRKTRIGEEFIFSLKAPISFSNAIRRIMISEVPTFAIEDVYIYENSSSMDDEILAHRLGLIPIKGKPLLENEVI
TFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGKGQRIQIECEAIPGIGKVHAKWQPCNAVYKQIADDEVEFFVETFG
QMEAEEILEEAVKILKNKAESFLQQLEMIEQ

Sequences:

>Translated_191_residues
MITIKEKRKTRIGEEFIFSLKAPISFSNAIRRIMISEVPTFAIEDVYIYENSSSMDDEILAHRLGLIPIKGKPLLENEVI
TFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGKGQRIQIECEAIPGIGKVHAKWQPCNAVYKQIADDEVEFFVETFG
QMEAEEILEEAVKILKNKAESFLQQLEMIEQ
>Mature_191_residues
MITIKEKRKTRIGEEFIFSLKAPISFSNAIRRIMISEVPTFAIEDVYIYENSSSMDDEILAHRLGLIPIKGKPLLENEVI
TFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGKGQRIQIECEAIPGIGKVHAKWQPCNAVYKQIADDEVEFFVETFG
QMEAEEILEEAVKILKNKAESFLQQLEMIEQ

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoD/eukaryotic RPB3 RNA polymerase subunit family

Homologues:

Organism=Homo sapiens, GI42560246, Length=168, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI4759046, Length=168, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25155095, Length=186, Percent_Identity=31.1827956989247, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322168, Length=178, Percent_Identity=32.5842696629214, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325367, Length=167, Percent_Identity=35.3293413173653, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17137650, Length=237, Percent_Identity=29.1139240506329, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129237, Length=188, Percent_Identity=31.3829787234043, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOD_METJA (Q57648)

Other databases:

- EMBL:   L77117
- PIR:   A64324
- RefSeq:   NP_247160.1
- ProteinModelPortal:   Q57648
- SMR:   Q57648
- IntAct:   Q57648
- GeneID:   1451040
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0192
- NMPDR:   fig|243232.1.peg.197
- TIGR:   MJ0192
- HOGENOM:   HBG539019
- OMA:   CNAVYKQ
- ProtClustDB:   PRK14979
- BioCyc:   MJAN243232:MJ_0192-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00320
- InterPro:   IPR001514
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR022842
- Gene3D:   G3DSA:2.170.120.12
- SMART:   SM00662

Pfam domain/function: PF01000 RNA_pol_A_bac; PF01193 RNA_pol_L; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 21699; Mature: 21699

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00446 RNA_POL_D_30KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIKEKRKTRIGEEFIFSLKAPISFSNAIRRIMISEVPTFAIEDVYIYENSSSMDDEIL
CCEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHH
AHRLGLIPIKGKPLLENEVITFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGKGQRI
HHHHCEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEECCCEEEECCCCEE
QIECEAIPGIGKVHAKWQPCNAVYKQIADDEVEFFVETFGQMEAEEILEEAVKILKNKAE
EEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SFLQQLEMIEQ
HHHHHHHHHCC
>Mature Secondary Structure
MITIKEKRKTRIGEEFIFSLKAPISFSNAIRRIMISEVPTFAIEDVYIYENSSSMDDEIL
CCEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHH
AHRLGLIPIKGKPLLENEVITFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGKGQRI
HHHHCEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEECCCEEEECCCCEE
QIECEAIPGIGKVHAKWQPCNAVYKQIADDEVEFFVETFGQMEAEEILEEAVKILKNKAE
EEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SFLQQLEMIEQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087