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The map label for this gene is eif2a

Identifier: 15668289

GI number: 15668289

Start: 114162

End: 114962

Strand: Reverse

Name: eif2a

Synonym: MJ0117

Alternate gene names: 15668289

Gene position: 114962-114162 (Counterclockwise)

Preceding gene: 15668291

Following gene: 15668288

Centisome position: 6.9

GC content: 36.95

Gene sequence:

>801_bases
GTGTTTATTATGAGAAGAGAATTTCCAGAAGAAGGAGATATAGTTATAGGAACTGTAAAGGATGTTAAGCCGTATGGAGC
ATTCGTAGAGCTTTTAGAATACCCAGGAAAGGAAGGAATGATTCACATCTCTGAGGTTACATCAGGATGGGTTAAAAACA
TTAGAGACCACGTTAAAGTTGGGCAGAGAGTTGTTGCAAAGGTTTTGAGAGTTGATGAGAGGAAGGGACATATTGATTTA
TCCTTAAAGAGAGTTACTGAGCAGCAAAAAAGGGCAAAAGTTCAAGAATGGAAGAGATTCCAAAGAGCTTCAAAGATGCT
TGAAAGAGCTGCTGAAAAATTGGGTAAAAGCTTAGAGGAAGCTTGGGAAGAGGTTGGCTATTTGTTGGAGGATGAGTTTG
GGGAGCTATACAATGCCTTTGAAACAATGGTTATTGAAGGGAAAGAAGTTTTAGATGATTTAGAGATTAGTGAAGAATGG
AAAAATGTTTTATATGAAGTAGCTAAGGAGAGTATTGAGCTAACAAACGTTGAAGTTGAAGGAGTTATTGAGATGAAATC
TTACGCCCCAGATGGAATTAAACAAATAAAGAAAGCATTAACAACAGCCTTAAAAGCTAACCCTTATGAGGATGTTGAGG
TTAAGATAACCTATATAGGAGCTCCAAAGTATAGGGTTGTTGTTATAGCTCCAGATTACAAGAGTGGAGAGGAGGTTTTT
AAAAAAGTTTGTGAAAAGGCAGTAGCAACAATTAAAAAACTTGGTGGAGAAGGAACTTACTATAGGGAGAGTAAGAAATA
A

Upstream 100 bases:

>100_bases
CTCTTAAACTCAAATAATGAAAAAGCAAATTCACCAATTCAATCTTTTTATATTACTACATTATAATTAATTTTAAAGGA
ACAGAATCTAATTTTATGAG

Downstream 100 bases:

>100_bases
AGATAAGTGGTAGAGATGAGAATGAAAAAATGTCCAAAATGCGGGCTATATACTTTAAAAGAAATCTGTCCAAAATGTGG
AGAGAAAACGGTAATTCCAA

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MFIMRREFPEEGDIVIGTVKDVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKVGQRVVAKVLRVDERKGHIDL
SLKRVTEQQKRAKVQEWKRFQRASKMLERAAEKLGKSLEEAWEEVGYLLEDEFGELYNAFETMVIEGKEVLDDLEISEEW
KNVLYEVAKESIELTNVEVEGVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKYRVVVIAPDYKSGEEVF
KKVCEKAVATIKKLGGEGTYYRESKK

Sequences:

>Translated_266_residues
MFIMRREFPEEGDIVIGTVKDVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKVGQRVVAKVLRVDERKGHIDL
SLKRVTEQQKRAKVQEWKRFQRASKMLERAAEKLGKSLEEAWEEVGYLLEDEFGELYNAFETMVIEGKEVLDDLEISEEW
KNVLYEVAKESIELTNVEVEGVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKYRVVVIAPDYKSGEEVF
KKVCEKAVATIKKLGGEGTYYRESKK
>Mature_266_residues
MFIMRREFPEEGDIVIGTVKDVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKVGQRVVAKVLRVDERKGHIDL
SLKRVTEQQKRAKVQEWKRFQRASKMLERAAEKLGKSLEEAWEEVGYLLEDEFGELYNAFETMVIEGKEVLDDLEISEEW
KNVLYEVAKESIELTNVEVEGVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKYRVVVIAPDYKSGEEVF
KKVCEKAVATIKKLGGEGTYYRESKK

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI4758256, Length=253, Percent_Identity=28.4584980237154, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17509871, Length=266, Percent_Identity=27.8195488721804, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322466, Length=259, Percent_Identity=32.046332046332, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18860087, Length=224, Percent_Identity=29.0178571428571, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): IF2A_METJA (Q57581)

Other databases:

- EMBL:   L77117
- PIR:   E64314
- RefSeq:   NP_247082.1
- ProteinModelPortal:   Q57581
- SMR:   Q57581
- GeneID:   1450960
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0117
- NMPDR:   fig|243232.1.peg.119
- TIGR:   MJ0117
- HOGENOM:   HBG496806
- OMA:   EYPGKEG
- ProtClustDB:   PRK03987
- BioCyc:   MJAN243232:MJ_0117-MONOMER
- HAMAP:   MF_00231
- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 30485; Mature: 30485

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIMRREFPEEGDIVIGTVKDVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKV
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHH
GQRVVAKVLRVDERKGHIDLSLKRVTEQQKRAKVQEWKRFQRASKMLERAAEKLGKSLEE
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWEEVGYLLEDEFGELYNAFETMVIEGKEVLDDLEISEEWKNVLYEVAKESIELTNVEVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEH
GVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKYRVVVIAPDYKSGEEVF
HEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHH
KKVCEKAVATIKKLGGEGTYYRESKK
HHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFIMRREFPEEGDIVIGTVKDVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKV
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHH
GQRVVAKVLRVDERKGHIDLSLKRVTEQQKRAKVQEWKRFQRASKMLERAAEKLGKSLEE
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWEEVGYLLEDEFGELYNAFETMVIEGKEVLDDLEISEEWKNVLYEVAKESIELTNVEVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEH
GVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKYRVVVIAPDYKSGEEVF
HEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHH
KKVCEKAVATIKKLGGEGTYYRESKK
HHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087