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The map label for this gene is argB

Identifier: 15668240

GI number: 15668240

Start: 66007

End: 66909

Strand: Direct

Name: argB

Synonym: MJ0069

Alternate gene names: 15668240

Gene position: 66007-66909 (Clockwise)

Preceding gene: 15668238

Following gene: 15668241

Centisome position: 3.96

GC content: 36.99

Gene sequence:

>903_bases
ATGGGGATGGACATGATAGAGATGATTGAAAAGGCAGAGATTTTAATGGAAGCTCTTCCATTCATACAGAAATTTTATGG
GAAGATTTTTGTCATAAAGTATGGCGGGCATGCGATGATTGATGAGAAGGCAAAGAATTGGACTGCTCAAGATGTTGTTT
TGTTGAAGTATGTTGGAATAAATCCAGTTGTAGTTCATGGTGGAGGTCCAGAAATCAACAAAGCAATGGAAAAAATGGGG
AAGAAACCAGAGTTTGTCCATGGGTTGAGAGTTACTGATGAAGAAACTTTAGATATTGTTGAAATGGTTTTAGCCGGAAA
GATTAATGGAGACATTGTCTCAAAGTTATCAAAGTTTGGTGGAAAGGCTGTTGGACTATCTGGAAAATCTGGAAGGATAA
TTTTAGCCAAGAAAAAATTAAAGAAAATAAAAACTGAAAAAGGGGAGGAGATAGAGGTTGATTTAGGTAGAGTTGGAGAG
ACAGTTGAGGTTAATACTGAACTATTAGAGATTTTGATAAACAACGGCTACATCCCAGTTGTATCACCAATTGGTTTGGA
TGAGAAGGGAGAGGCATATAATTTAAATGCCGATACCGTTGCTGGAGACATAGCTGGAGCTTTGAAGGCGGAGAAGCTTA
TTTTAATAACAGATGTTGATGGAATAATGGATGATATAAATAATCCAGAGACGTTGCATAGAAAATTAACAGCTTCAGAA
CTAAAAGAAATGATAGAAGATGGAAGAATAAAGGGAGGGATGATTCCAAAGGCTGAAAGTGCCTTATATGCCTTAGAGCA
TGGAGTTAAGAGCGTTCATATAATAAATGGAAAGATTCCTCATGCTTTGTTGTTGGAGATATTTACAGAGGAGGGTATTG
GGACGATGATAACAAGAGATTAA

Upstream 100 bases:

>100_bases
ATTTTTATTAATTACCTTAGTTACGGCTTCTATTATTTAAAGTTATTGTTTGATAAATTTTAAATAATTCCTTTATCCAC
TCATAAACTTAAAAGGTTTT

Downstream 100 bases:

>100_bases
AGTTTTTATATTATAAACTACTTAAGAATTAAAATAAGACAAATAAGGGGATAACTATGCTCAATATAAACAAAGAGATA
GCACAAATAGAAACTGAATT

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MGMDMIEMIEKAEILMEALPFIQKFYGKIFVIKYGGHAMIDEKAKNWTAQDVVLLKYVGINPVVVHGGGPEINKAMEKMG
KKPEFVHGLRVTDEETLDIVEMVLAGKINGDIVSKLSKFGGKAVGLSGKSGRIILAKKKLKKIKTEKGEEIEVDLGRVGE
TVEVNTELLEILINNGYIPVVSPIGLDEKGEAYNLNADTVAGDIAGALKAEKLILITDVDGIMDDINNPETLHRKLTASE
LKEMIEDGRIKGGMIPKAESALYALEHGVKSVHIINGKIPHALLLEIFTEEGIGTMITRD

Sequences:

>Translated_300_residues
MGMDMIEMIEKAEILMEALPFIQKFYGKIFVIKYGGHAMIDEKAKNWTAQDVVLLKYVGINPVVVHGGGPEINKAMEKMG
KKPEFVHGLRVTDEETLDIVEMVLAGKINGDIVSKLSKFGGKAVGLSGKSGRIILAKKKLKKIKTEKGEEIEVDLGRVGE
TVEVNTELLEILINNGYIPVVSPIGLDEKGEAYNLNADTVAGDIAGALKAEKLILITDVDGIMDDINNPETLHRKLTASE
LKEMIEDGRIKGGMIPKAESALYALEHGVKSVHIINGKIPHALLLEIFTEEGIGTMITRD
>Mature_299_residues
GMDMIEMIEKAEILMEALPFIQKFYGKIFVIKYGGHAMIDEKAKNWTAQDVVLLKYVGINPVVVHGGGPEINKAMEKMGK
KPEFVHGLRVTDEETLDIVEMVLAGKINGDIVSKLSKFGGKAVGLSGKSGRIILAKKKLKKIKTEKGEEIEVDLGRVGET
VEVNTELLEILINNGYIPVVSPIGLDEKGEAYNLNADTVAGDIAGALKAEKLILITDVDGIMDDINNPETLHRKLTASEL
KEMIEDGRIKGGMIPKAESALYALEHGVKSVHIINGKIPHALLLEIFTEEGIGTMITRD

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=299, Percent_Identity=29.0969899665552, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693208, Length=270, Percent_Identity=32.2222222222222, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320913, Length=272, Percent_Identity=28.6764705882353, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_METJA (Q60382)

Other databases:

- EMBL:   L77117
- PIR:   E64308
- RefSeq:   NP_247033.1
- ProteinModelPortal:   Q60382
- SMR:   Q60382
- GeneID:   1450908
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0069
- NMPDR:   fig|243232.1.peg.70
- TIGR:   MJ0069
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- ProtClustDB:   PRK00942
- BioCyc:   MJAN243232:MJ_0069-MONOMER
- BRENDA:   2.7.2.8
- GO:   GO:0005737
- HAMAP:   MF_00082_A
- InterPro:   IPR004662
- InterPro:   IPR001048
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 32726; Mature: 32595

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: BINDING 90-90 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMDMIEMIEKAEILMEALPFIQKFYGKIFVIKYGGHAMIDEKAKNWTAQDVVLLKYVGI
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHCCCCHHHEEEEEEECC
NPVVVHGGGPEINKAMEKMGKKPEFVHGLRVTDEETLDIVEMVLAGKINGDIVSKLSKFG
CEEEEECCCCHHHHHHHHHCCCCCCEECEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GKAVGLSGKSGRIILAKKKLKKIKTEKGEEIEVDLGRVGETVEVNTELLEILINNGYIPV
CCEEECCCCCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHHHHHHHCCCCEEE
VSPIGLDEKGEAYNLNADTVAGDIAGALKAEKLILITDVDGIMDDINNPETLHRKLTASE
ECCCCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHH
LKEMIEDGRIKGGMIPKAESALYALEHGVKSVHIINGKIPHALLLEIFTEEGIGTMITRD
HHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
GMDMIEMIEKAEILMEALPFIQKFYGKIFVIKYGGHAMIDEKAKNWTAQDVVLLKYVGI
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHCCCCHHHEEEEEEECC
NPVVVHGGGPEINKAMEKMGKKPEFVHGLRVTDEETLDIVEMVLAGKINGDIVSKLSKFG
CEEEEECCCCHHHHHHHHHCCCCCCEECEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GKAVGLSGKSGRIILAKKKLKKIKTEKGEEIEVDLGRVGETVEVNTELLEILINNGYIPV
CCEEECCCCCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHHHHHHHCCCCEEE
VSPIGLDEKGEAYNLNADTVAGDIAGALKAEKLILITDVDGIMDDINNPETLHRKLTASE
ECCCCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHH
LKEMIEDGRIKGGMIPKAESALYALEHGVKSVHIINGKIPHALLLEIFTEEGIGTMITRD
HHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087