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The map label for this gene is rtcA

Identifier: 15668196

GI number: 15668196

Start: 26106

End: 27122

Strand: Reverse

Name: rtcA

Synonym: MJ0025

Alternate gene names: 15668196

Gene position: 27122-26106 (Counterclockwise)

Preceding gene: 15668197

Following gene: 15668195

Centisome position: 1.63

GC content: 32.35

Gene sequence:

>1017_bases
ATGGATTTTATTGTTATTGATGGAAGTTACTTAGAAGGAGGAGGGCAGATTATAAGAACTGCTGTTTCTTTATCAGCTTT
AACTCAAAAACCAGTAAAAATTATTAACATAAGGAAAAAGAGAAAGAATAAAGGTTTAGCTCCTCAACATGTATCTGCAG
TTAAAGCAGTAAAAAAGCTTTGCAATGCTGAAGTTTTTGGATTAAACGTTGGCTCAGAAGAATTAACTTTTATACCTTCA
AAATTATCTCCAAAGGATTTTACAATTGATATTGGAACTGCTGGGAGCATATCTTTGGTTATACAAACTCTCCTCCCATT
ATCATTAGGAATTAACAAAAAATTCACTGTAAAAATAAAGGGAGGGACTGATGTCAAAAGAGCCCCACCAATTGATTATG
TAAAAAATGTAACCTTAAAAATTCTTAGAAATTTTGGAGTATTGACAGAGCTAAAAGTTTTAAAAAGAGGATTTTATCCA
GAAGGCGGAGGAGAGGTTATTTTTGAAGTAAAGCCTTCAAAAATTAAAAAATTTGATTTAATAGAACATTCTAAAAGTAA
CTTAGTTGAAGGAATTAGCTATGTGCAAAATTTAGATGAGAGTATAGCAAGAAGAATGAGAAAAAAGGCAGTTGATTTAT
TAAACAAAGAAAAACTTCTGCCCAATATAAAAATAGAATGTTCAAAGGGTATTTCTACTGGAGCAGGGATAGTTTTATGG
AACGATACTTTAGGGGGAAGTTGTTTAGGAGAGAAAGGGTTAAGGGCGGAGATTGTTGCTGAAAGGGCGGTTAATGAGTT
ATTAAAGGAGAGGGAAAGTGGGATGGCTTTAGATAAATATATGGGAGACCAAATAATCCCATTCTTAGCTTTTGGTAAAG
GAATAGTGGGGGTTTCAGAGATAACCAATCATACAAAAACAAACATGTGGGTTGTTAAACACTTTTTGGATGTAGATTTT
GAGATTAAAGAATATAAAGAAAATAATTGCAATGGATTTACTATTGAGGTGGTTTAA

Upstream 100 bases:

>100_bases
TTAATATAAAAGAGGGATATATAAAAGGAACTTTAAGAGTTTTTCCACCAAATGAACCATTTTTTATTGCAAAATTGAGA
AAAATATAATTGGTGATGAG

Downstream 100 bases:

>100_bases
ATGTTTAATTATTTTGAAACTACTGCTGATTTGGGTGTTGAAGCAAAAGGAAAGAGTTTAGAAGAGGCATTTAAAGAAGG
AGCTAAGGGACTTTACAATA

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MDFIVIDGSYLEGGGQIIRTAVSLSALTQKPVKIINIRKKRKNKGLAPQHVSAVKAVKKLCNAEVFGLNVGSEELTFIPS
KLSPKDFTIDIGTAGSISLVIQTLLPLSLGINKKFTVKIKGGTDVKRAPPIDYVKNVTLKILRNFGVLTELKVLKRGFYP
EGGGEVIFEVKPSKIKKFDLIEHSKSNLVEGISYVQNLDESIARRMRKKAVDLLNKEKLLPNIKIECSKGISTGAGIVLW
NDTLGGSCLGEKGLRAEIVAERAVNELLKERESGMALDKYMGDQIIPFLAFGKGIVGVSEITNHTKTNMWVVKHFLDVDF
EIKEYKENNCNGFTIEVV

Sequences:

>Translated_338_residues
MDFIVIDGSYLEGGGQIIRTAVSLSALTQKPVKIINIRKKRKNKGLAPQHVSAVKAVKKLCNAEVFGLNVGSEELTFIPS
KLSPKDFTIDIGTAGSISLVIQTLLPLSLGINKKFTVKIKGGTDVKRAPPIDYVKNVTLKILRNFGVLTELKVLKRGFYP
EGGGEVIFEVKPSKIKKFDLIEHSKSNLVEGISYVQNLDESIARRMRKKAVDLLNKEKLLPNIKIECSKGISTGAGIVLW
NDTLGGSCLGEKGLRAEIVAERAVNELLKERESGMALDKYMGDQIIPFLAFGKGIVGVSEITNHTKTNMWVVKHFLDVDF
EIKEYKENNCNGFTIEVV
>Mature_338_residues
MDFIVIDGSYLEGGGQIIRTAVSLSALTQKPVKIINIRKKRKNKGLAPQHVSAVKAVKKLCNAEVFGLNVGSEELTFIPS
KLSPKDFTIDIGTAGSISLVIQTLLPLSLGINKKFTVKIKGGTDVKRAPPIDYVKNVTLKILRNFGVLTELKVLKRGFYP
EGGGEVIFEVKPSKIKKFDLIEHSKSNLVEGISYVQNLDESIARRMRKKAVDLLNKEKLLPNIKIECSKGISTGAGIVLW
NDTLGGSCLGEKGLRAEIVAERAVNELLKERESGMALDKYMGDQIIPFLAFGKGIVGVSEITNHTKTNMWVVKHFLDVDF
EIKEYKENNCNGFTIEVV

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=336, Percent_Identity=33.0357142857143, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI195927044, Length=349, Percent_Identity=31.8051575931232, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI157426877, Length=334, Percent_Identity=25.4491017964072, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI48994937, Length=327, Percent_Identity=34.5565749235474, Blast_Score=178, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI32564710, Length=337, Percent_Identity=24.0356083086053, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324564, Length=299, Percent_Identity=24.7491638795987, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI18543269, Length=333, Percent_Identity=33.6336336336336, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI18860065, Length=336, Percent_Identity=23.2142857142857, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_METJA (Q60335)

Other databases:

- EMBL:   L77117
- PIR:   A64303
- RefSeq:   NP_246988.1
- ProteinModelPortal:   Q60335
- SMR:   Q60335
- GeneID:   1450863
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0025
- NMPDR:   fig|243232.1.peg.25
- TIGR:   MJ0025
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   PRK04204
- BioCyc:   MJAN243232:MJ_0025-MONOMER
- BRENDA:   6.5.1.4
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 37229; Mature: 37229

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 304-304 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIVIDGSYLEGGGQIIRTAVSLSALTQKPVKIINIRKKRKNKGLAPQHVSAVKAVKKL
CCEEEEECCEECCCHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCCCHHHHHHHHHHHHH
CNAEVFGLNVGSEELTFIPSKLSPKDFTIDIGTAGSISLVIQTLLPLSLGINKKFTVKIK
HCCEEEEEECCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEC
GGTDVKRAPPIDYVKNVTLKILRNFGVLTELKVLKRGFYPEGGGEVIFEVKPSKIKKFDL
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
IEHSKSNLVEGISYVQNLDESIARRMRKKAVDLLNKEKLLPNIKIECSKGISTGAGIVLW
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEE
NDTLGGSCLGEKGLRAEIVAERAVNELLKERESGMALDKYMGDQIIPFLAFGKGIVGVSE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCHHH
ITNHTKTNMWVVKHFLDVDFEIKEYKENNCNGFTIEVV
HHCCCCCHHHHHHHHHCCCCEEEHHCCCCCCEEEEEEC
>Mature Secondary Structure
MDFIVIDGSYLEGGGQIIRTAVSLSALTQKPVKIINIRKKRKNKGLAPQHVSAVKAVKKL
CCEEEEECCEECCCHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCCCHHHHHHHHHHHHH
CNAEVFGLNVGSEELTFIPSKLSPKDFTIDIGTAGSISLVIQTLLPLSLGINKKFTVKIK
HCCEEEEEECCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEC
GGTDVKRAPPIDYVKNVTLKILRNFGVLTELKVLKRGFYPEGGGEVIFEVKPSKIKKFDL
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
IEHSKSNLVEGISYVQNLDESIARRMRKKAVDLLNKEKLLPNIKIECSKGISTGAGIVLW
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEE
NDTLGGSCLGEKGLRAEIVAERAVNELLKERESGMALDKYMGDQIIPFLAFGKGIVGVSE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCHHH
ITNHTKTNMWVVKHFLDVDFEIKEYKENNCNGFTIEVV
HHCCCCCHHHHHHHHHCCCCEEEHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087