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The map label for this gene is ksgA

Identifier: 15646040

GI number: 15646040

Start: 1501517

End: 1502332

Strand: Reverse

Name: ksgA

Synonym: HP1431

Alternate gene names: 15646040

Gene position: 1502332-1501517 (Counterclockwise)

Preceding gene: 15646042

Following gene: 15646039

Centisome position: 90.08

GC content: 39.34

Gene sequence:

>816_bases
ATGGTAGTAGCTAAAAAGTCTTTAGGACAGCATTTTTTAACGGACGAGTCGTTTTTAGACAGAATCGTTAATGCTTTGCC
CCCCTTAAACCCGTTGAAATTAGTTGAAATTGGCGTGGGGCTAGGGGATTTGACTCTTAAGTTGTTGGATCGCTATCCTT
TAAAAACTTATGAGATAGACAGCCATTTGTGCGAGAAAATGCGATCAAAGCTAAAGGCACAAAAAAAGCCTTTTAAATTA
GAATTAGTGGAAAAAGACGCTCTTTTTTTAAAAGAAGAAGAGCCTTATTTTTTGATCTCTAATTTGCCTTATTATATCGC
TACCAGGCTTGTTTTAAACGCGTTCAAAGACCCTAAATGCAGGGGCTTATTGGTGATGACGCAAAAGGAAGTGGCGCTCA
AGTTTTGCGCTAAAGATTCACAGAACGCTTTAAGCGTTTTAGCACACACGATAGGGAACGCTACCCTTTTGTTTGATGTG
CCACCTAGCGCGTTTAGCCCGCCTCCAAAGGTGTTTTCTAGCGTGTTTGAAGTGATCAAAGAGCCGCTGAAAGAAAAGGC
GTTGGCTTCATTAGCCCAAGCGCCATTTTTTGAAGAAGCCCTACAAAAAGGGTTTGAAATGTTAGAAGATTTTTTGAAAG
CTTGTTTCTCATCTCCCAGGAAGACGCTTTCAAACAATCTTAAAAAAAGCGTTTCTTATAGAGAAAAGCTTGATAAGGTG
TTAGATTTTCTAGCGTTAGAAAACCAACCAACAAGCGTGAGAGCGTCTGAGATAAAAGATTATCTCAAGCTCTTAAATTA
CCTTTTAAAAGGCTAA

Upstream 100 bases:

>100_bases
ATCTTTGAATGAAAAAAGCTATAATAAAGAAGTTGCATTAAAAAGGAAAGACAAATCCCCTTTTTAAGGTATAATATGAG
GAATTTTAGAAGCGTTTCTT

Downstream 100 bases:

>100_bases
TGCCTTTTTAAAAAATTGAAATAAAGGAATAAAAGTTATGACGGATAACAACCAAAACAATGAAAACCATGAAAACAGCA
GTGAAAATTCAAAAGCTGAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVVAKKSLGQHFLTDESFLDRIVNALPPLNPLKLVEIGVGLGDLTLKLLDRYPLKTYEIDSHLCEKMRSKLKAQKKPFKL
ELVEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKCRGLLVMTQKEVALKFCAKDSQNALSVLAHTIGNATLLFDV
PPSAFSPPPKVFSSVFEVIKEPLKEKALASLAQAPFFEEALQKGFEMLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
LDFLALENQPTSVRASEIKDYLKLLNYLLKG

Sequences:

>Translated_271_residues
MVVAKKSLGQHFLTDESFLDRIVNALPPLNPLKLVEIGVGLGDLTLKLLDRYPLKTYEIDSHLCEKMRSKLKAQKKPFKL
ELVEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKCRGLLVMTQKEVALKFCAKDSQNALSVLAHTIGNATLLFDV
PPSAFSPPPKVFSSVFEVIKEPLKEKALASLAQAPFFEEALQKGFEMLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
LDFLALENQPTSVRASEIKDYLKLLNYLLKG
>Mature_271_residues
MVVAKKSLGQHFLTDESFLDRIVNALPPLNPLKLVEIGVGLGDLTLKLLDRYPLKTYEIDSHLCEKMRSKLKAQKKPFKL
ELVEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKCRGLLVMTQKEVALKFCAKDSQNALSVLAHTIGNATLLFDV
PPSAFSPPPKVFSSVFEVIKEPLKEKALASLAQAPFFEEALQKGFEMLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
LDFLALENQPTSVRASEIKDYLKLLNYLLKG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=284, Percent_Identity=24.6478873239437, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI7657198, Length=277, Percent_Identity=24.5487364620939, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1786236, Length=283, Percent_Identity=30.0353356890459, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=266, Percent_Identity=28.1954887218045, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21357273, Length=241, Percent_Identity=29.045643153527, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358017, Length=284, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30644; Mature: 30644

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAKKSLGQHFLTDESFLDRIVNALPPLNPLKLVEIGVGLGDLTLKLLDRYPLKTYEID
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
SHLCEKMRSKLKAQKKPFKLELVEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKC
HHHHHHHHHHHHHHCCCCEEEEECCCHHHEECCCCEEEECCCHHHHHHHHHHHHCCCCCC
RGLLVMTQKEVALKFCAKDSQNALSVLAHTIGNATLLFDVPPSAFSPPPKVFSSVFEVIK
CEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EPLKEKALASLAQAPFFEEALQKGFEMLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LDFLALENQPTSVRASEIKDYLKLLNYLLKG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVAKKSLGQHFLTDESFLDRIVNALPPLNPLKLVEIGVGLGDLTLKLLDRYPLKTYEID
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
SHLCEKMRSKLKAQKKPFKLELVEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKC
HHHHHHHHHHHHHHCCCCEEEEECCCHHHEECCCCEEEECCCHHHHHHHHHHHHCCCCCC
RGLLVMTQKEVALKFCAKDSQNALSVLAHTIGNATLLFDVPPSAFSPPPKVFSSVFEVIK
CEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EPLKEKALASLAQAPFFEEALQKGFEMLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LDFLALENQPTSVRASEIKDYLKLLNYLLKG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA