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The map label for this gene is pyrB

Identifier: 15645698

GI number: 15645698

Start: 1143527

End: 1144450

Strand: Reverse

Name: pyrB

Synonym: HP1084

Alternate gene names: 15645698

Gene position: 1144450-1143527 (Counterclockwise)

Preceding gene: 15645707

Following gene: 15645697

Centisome position: 68.62

GC content: 41.99

Gene sequence:

>924_bases
ATGCCAAAAAAATGCCGACACTTGCTCCAAACCAGCGATTTAAGCCTAGATGAAATCAAGCTTTTATTAGAAAAAGCGAG
CGTTTATGCGAACGATTTTAACGCCGTATCTTTAGAAACAAAAGAAAAAATGCACAATAAAATCATCGTGGCTTTATTTT
TTGAAAATTCCACCAGAACGGTGTCTAGTTTTGAAATCGCAAGCCTAAGGTTGGGGGCAAAAATAGTGAAATTAAACATG
CAAACAAGCTCTGCTTCAAAGGGTGAAACTCTAACAGACACTTTCAAAAATATCCATGCCATGCAGCCTGACGCTATCAT
CACACGGCATGCTTTTTCAAGCGCGCCTTTTAAATTGGCTGAATTTTCACAATGCCCCTTGATTAACGCAGGAAGCGGCG
TGAGCGCTCATCCTACCCAAGCGTTATTAGACTTGCTCACCCTTTATCAGCATTTTGGCAGTTTGGAAAATTTAAAAGGG
AAGAAAATCGCTTTCATAGGCGATGTGAAAAATTCCAGAGTGGCTAATAGCAACATTAAATTGCTCCAAAGGCTAGGGCT
TGAGATCATGCTGTGCGCTCCAAGCTCCATGCTCCCTAGCGTTTCTTTAAAAACGACGCACAACGTTGAAGAAGCGATAG
CATTTGCAGACATTTTAATGAGTTTGAGGACCCAAACCGAACGGCACAATACGCCCATTTTTGCGAGCTTGAAAGATTAT
GGCAACGCTTATTGCATCACCCAACAACGCCTAAAGGCTCACGCTAAAAATAAAGAGGTGATTATTTTACACCCAGGCCC
GGTGCATAGGGATATTGATATAGAAAGCGCGGTGTTAGAAGATGAGCGATCTAAAGTTTTAGAGCAAGTCAAAAATGGCG
TGGCAATGCGCATGGCGGTGTTGGAGTTTTTGCTATTAGATTGA

Upstream 100 bases:

>100_bases
ACACCAGAAGTGTTGAGAGATTTTTCAAGCCCATTCCTTGCTAAAACCCATGCCATTTTTAGCTAAAATAAAATCTATAT
TATAACTAAAAGGGGGTTTT

Downstream 100 bases:

>100_bases
TTGTCAAACTTAAAAAATGCTTTTTGGCGTTTTTTTAAAAAATGGGTTTGGTTATAAAACGGCACAAAAACCCTAATTTG
ATTAAGTTTTGATTTACTTT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPKKCRHLLQTSDLSLDEIKLLLEKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNM
QTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSVSLKTTHNVEEAIAFADILMSLRTQTERHNTPIFASLKDY
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAVLEFLLLD

Sequences:

>Translated_307_residues
MPKKCRHLLQTSDLSLDEIKLLLEKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNM
QTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSVSLKTTHNVEEAIAFADILMSLRTQTERHNTPIFASLKDY
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAVLEFLLLD
>Mature_306_residues
PKKCRHLLQTSDLSLDEIKLLLEKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNMQ
TSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGK
KIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSVSLKTTHNVEEAIAFADILMSLRTQTERHNTPIFASLKDYG
NAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAVLEFLLLD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=276, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI2367364, Length=323, Percent_Identity=33.1269349845201, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790703, Length=330, Percent_Identity=26.0606060606061, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI48994908, Length=250, Percent_Identity=30, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786469, Length=167, Percent_Identity=31.7365269461078, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=31.2883435582822, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=275, Percent_Identity=36, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322373, Length=329, Percent_Identity=25.2279635258359, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24642586, Length=283, Percent_Identity=33.2155477031802, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34068; Mature: 33937

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKCRHLLQTSDLSLDEIKLLLEKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCEEEEEEEECCCCCE
VSSFEIASLRLGAKIVKLNMQTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLA
EHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHH
EFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPSVSLKTTHNVEEAIAFADILMSLRTQTERHNTPIFASLKDY
HHHHCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHC
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAV
CCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEFLLLD
HHHHHCC
>Mature Secondary Structure 
PKKCRHLLQTSDLSLDEIKLLLEKASVYANDFNAVSLETKEKMHNKIIVALFFENSTRT
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCEEEEEEEECCCCCE
VSSFEIASLRLGAKIVKLNMQTSSASKGETLTDTFKNIHAMQPDAIITRHAFSSAPFKLA
EHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHH
EFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPSVSLKTTHNVEEAIAFADILMSLRTQTERHNTPIFASLKDY
HHHHCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHC
GNAYCITQQRLKAHAKNKEVIILHPGPVHRDIDIESAVLEDERSKVLEQVKNGVAMRMAV
CCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEFLLLD
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA