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The map label for this gene is ruvC

Identifier: 15645496

GI number: 15645496

Start: 929787

End: 930260

Strand: Reverse

Name: ruvC

Synonym: HP0877

Alternate gene names: 15645496

Gene position: 930260-929787 (Counterclockwise)

Preceding gene: 15645499

Following gene: 15645494

Centisome position: 55.78

GC content: 42.41

Gene sequence:

>474_bases
ATGCGTATTTTAGGAATAGATCCGGGCAGTAGGAAATGCGGGTATGCTATCATTTCTCACGCTTCTAACAAGCTTTCTTT
AATCACGGCCGGGTTCATTAATATCACCACGACACGCTTGCAAGAACAGATTTTAGATTTGATAGAAGCCTTAGATTGCT
TATTGGATCGTTACGAAGTCAATGAAGTGGCGATTGAAGATATTTTCTTTGGGTATAACCCAAAAAGCGTGATCAAGCTC
GCGCAATTCAGGGGAGCGTTGTCCTTAAAGATTTTAGAAAGGATTGGTAATTTTAGCGAATACACGCCCTTACAAGTCAA
AAAAGCCCTGACCGGTAACGGGAAAGCCGCTAAAGAACAAGTAGCTTTTATGGTCAAGCGCTTGCTTAACATCACAAGCG
AAATCAAGCCTTTGGATATTAGCGATGCGATAGCCGTTGCTATCACGCATGCGCAACGCTTAAAGCTCCATTAA

Upstream 100 bases:

>100_bases
TTTAAAATTCTTAATGCAATCTTGTATTTTATTCTAACAAGAAACATGGATATAATAATAGCGTTTTTTAAACCTTGCAA
TTAAATAATAGGAATGGTTG

Downstream 100 bases:

>100_bases
CTACCATTTGTAAGCCACTTCAAAACGCGCGTTAAACCCAGGCTCAGCGATCGCTCTTTTAACAGCGTCAGTGCCTGGCA
TATCCGGGCTCATGACATAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRILGIDPGSRKCGYAIISHASNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVNEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH

Sequences:

>Translated_157_residues
MRILGIDPGSRKCGYAIISHASNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVNEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH
>Mature_157_residues
MRILGIDPGSRKCGYAIISHASNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVNEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=131, Percent_Identity=32.824427480916, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17425; Mature: 17425

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGSRKCGYAIISHASNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEV
CEEECCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEVAIEDIFFGYNPKSVIKLAQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQ
HHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHH
VAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRILGIDPGSRKCGYAIISHASNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEV
CEEECCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEVAIEDIFFGYNPKSVIKLAQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQ
HHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHH
VAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA