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Identifier: 15645469

GI number: 15645469

Start: 901118

End: 902701

Strand: Reverse

Name: Not Available

Synonym: HP0850

Alternate gene names: 15645469

Gene position: 902701-901118 (Counterclockwise)

Preceding gene: 15645471

Following gene: 15645468

Centisome position: 54.12

GC content: 41.22

Gene sequence:

>1584_bases
ATGGAAAACAACCCAAACAATAACCAAGCGTCATTAGAACGCAACGAATTGCACAACACCATTTGGAAAGTGGCTAACGA
ATTGAGAGGCTCAGTGGATGGCTGGGATTTTAAGCAATACGTTTTAGGCATTCTTTTTTACCGCTACATTTCAGAAAACA
TGACTCATTACATCAATAAAGAAGAGCGAAAGCGCGATCCGAGTTTTGATTACGCTAAATTAAGCGATGAAAAGGCCGAG
CGTGGAAGAAAACACCTTATTGAACAAAAAGGCTTTTTCATCCCGCCAAGCGCTTTATTTTGTAATGCGCTAAAAAACGC
GTGCCATAACGAAGATCTCAATGTAACCTTACAAAATATTTTTAACGAAATAGAAAAATCTAGCCTTGGCACTCCATCAG
AAGAAAATGTCAAAGGCTTGTTTGCGGATTTAGATGTCAATAGCAATAAATTAGGGAGCTCTCATCAAAATAGGGTGGAA
AAATTGACTAAAATCCTTGAAGCCATAGGGGGCATGCAATTAGGCGATTATTTAAAAAGCGGGATTGATGTTTTTGGCGA
CGCTTATGAATATTTAATGGCCATGTATGCGAGCAACGCCGGCAAAAGCGGAGGGGAATTTTTCACCCCCCAAGAAGTGA
GCGAACTGCTCGCTAAAATCACCCTACACGGCCAAGAGAGCGTCAATAAAGTTTATGACCCATGCTGTGGGAGTGGGAGC
TTACTCTTACAATTTTCTAAAGTGTTAGGCGATAAAAATGTCTCAAAAGGGTATTTTGGGCAAGAAATCAATTTGACCAC
TTACAACCTTTGCCGTATCAACATGTTTTTGCATGACATCAATTATTCTAAATTCCATATTGCGCACGGGGACACGCTTT
TAGACCCAAAACATGAAGACGATGAGCCTTTTGATGCGATCGTTTCCAACCCTCCTTATTCCACTAAATGGGTGGGCGAT
AGCAACCCCATTTTAATCAACGATGAGCGCTTTAGCCCGGCTGGTGTGCTAGCGCCCAAAAACGCCGCCGATCTCGCATT
CACTATGCACATGCTTTCTTATTTATCCAATAGCGGCACGGCTGCGATCGTGGAATTTCCCGGGGTGCTTTATAGGGGAA
ACGCTGAAGCAAAAATCAGAGAATATTTAGTCAAAGAGAATGTCATTGACTGCGTGATCGCTTTACCAGACAACCTCTTT
TTTGGAACGAGTATCGCTACTTGCATTTTAGTGCTTAAGAAAAACAAACAAGACGACACCACGCTTTTTATTGATGCGAG
TAAGGAATTTGTCAAAGAGGGCAAGAAAAACAAGCTCAAAGAGCGCAACCGAGAAAAGATTTTGCAAACCTATATTGAAA
GAAAAGAGATTAAGCATTTTTGCGCCCTAGCTAATATTGAGAAAATCAAAGAAAACGATTACAATCTCTCCGTGAATCGC
TATGTGGAGCAAGAAGACACCAAAGAAGCCATTGACATTAAAGCGCTTAATAGTGAGATTGCTCAAATCGTAGAAAAGCA
AAGCGCTTTAAGGAACCGTCTTGAATCCATCATCAAAGAGTTAGAAGGGGGGCAAAATGCATAA

Upstream 100 bases:

>100_bases
AAGGAATAAAAAAATAATATTCTTTGCTATAATGAAACCTTTAAAGGCTGACAACGCCAAACAGAACCGCCAATCAAAAC
ACAAAAAAGGGGCTTATATC

Downstream 100 bases:

>100_bases
AATAGAGCAACTGCTCCAAACTCTAGCGCCTAAGGGGGTGGAGTTTAGGAAGTTGGGGGATATTATCTTATCGTTAAAAA
CCGGATTAAATCCAAGAAAA

Product: type I restriction enzyme M protein (hsdM)

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MENNPNNNQASLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKEERKRDPSFDYAKLSDEKAE
RGRKHLIEQKGFFIPPSALFCNALKNACHNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHQNRVE
KLTKILEAIGGMQLGDYLKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHGQESVNKVYDPCCGSGS
LLLQFSKVLGDKNVSKGYFGQEINLTTYNLCRINMFLHDINYSKFHIAHGDTLLDPKHEDDEPFDAIVSNPPYSTKWVGD
SNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLF
FGTSIATCILVLKKNKQDDTTLFIDASKEFVKEGKKNKLKERNREKILQTYIERKEIKHFCALANIEKIKENDYNLSVNR
YVEQEDTKEAIDIKALNSEIAQIVEKQSALRNRLESIIKELEGGQNA

Sequences:

>Translated_527_residues
MENNPNNNQASLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKEERKRDPSFDYAKLSDEKAE
RGRKHLIEQKGFFIPPSALFCNALKNACHNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHQNRVE
KLTKILEAIGGMQLGDYLKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHGQESVNKVYDPCCGSGS
LLLQFSKVLGDKNVSKGYFGQEINLTTYNLCRINMFLHDINYSKFHIAHGDTLLDPKHEDDEPFDAIVSNPPYSTKWVGD
SNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLF
FGTSIATCILVLKKNKQDDTTLFIDASKEFVKEGKKNKLKERNREKILQTYIERKEIKHFCALANIEKIKENDYNLSVNR
YVEQEDTKEAIDIKALNSEIAQIVEKQSALRNRLESIIKELEGGQNA
>Mature_527_residues
MENNPNNNQASLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKEERKRDPSFDYAKLSDEKAE
RGRKHLIEQKGFFIPPSALFCNALKNACHNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHQNRVE
KLTKILEAIGGMQLGDYLKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHGQESVNKVYDPCCGSGS
LLLQFSKVLGDKNVSKGYFGQEINLTTYNLCRINMFLHDINYSKFHIAHGDTLLDPKHEDDEPFDAIVSNPPYSTKWVGD
SNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLF
FGTSIATCILVLKKNKQDDTTLFIDASKEFVKEGKKNKLKERNREKILQTYIERKEIKHFCALANIEKIKENDYNLSVNR
YVEQEDTKEAIDIKALNSEIAQIVEKQSALRNRLESIIKELEGGQNA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=249, Percent_Identity=24.0963855421687, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 59534; Mature: 59534

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNPNNNQASLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EERKRDPSFDYAKLSDEKAERGRKHLIEQKGFFIPPSALFCNALKNACHNEDLNVTLQNI
HHHCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHH
FNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHQNRVEKLTKILEAIGGMQLGDYLKS
HHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHGQESVNKVYDPCCGSGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCH
LLLQFSKVLGDKNVSKGYFGQEINLTTYNLCRINMFLHDINYSKFHIAHGDTLLDPKHED
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEHEEEEEHHEECCCCEEEEECCCCCCCCCCCC
DEPFDAIVSNPPYSTKWVGDSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGT
CCCHHHHHCCCCCCCEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
AAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLFFGTSIATCILVLKKNKQDDT
EEEEECCCEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
TLFIDASKEFVKEGKKNKLKERNREKILQTYIERKEIKHFCALANIEKIKENDYNLSVNR
EEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHH
YVEQEDTKEAIDIKALNSEIAQIVEKQSALRNRLESIIKELEGGQNA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MENNPNNNQASLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EERKRDPSFDYAKLSDEKAERGRKHLIEQKGFFIPPSALFCNALKNACHNEDLNVTLQNI
HHHCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHH
FNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHQNRVEKLTKILEAIGGMQLGDYLKS
HHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHGQESVNKVYDPCCGSGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCH
LLLQFSKVLGDKNVSKGYFGQEINLTTYNLCRINMFLHDINYSKFHIAHGDTLLDPKHED
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEHEEEEEHHEECCCCEEEEECCCCCCCCCCCC
DEPFDAIVSNPPYSTKWVGDSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGT
CCCHHHHHCCCCCCCEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
AAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLFFGTSIATCILVLKKNKQDDT
EEEEECCCEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
TLFIDASKEFVKEGKKNKLKERNREKILQTYIERKEIKHFCALANIEKIKENDYNLSVNR
EEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHH
YVEQEDTKEAIDIKALNSEIAQIVEKQSALRNRLESIIKELEGGQNA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]