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The map label for this gene is tig

Identifier: 15645414

GI number: 15645414

Start: 851625

End: 852980

Strand: Reverse

Name: tig

Synonym: HP0795

Alternate gene names: 15645414

Gene position: 852980-851625 (Counterclockwise)

Preceding gene: 15645415

Following gene: 15645413

Centisome position: 51.14

GC content: 37.39

Gene sequence:

>1356_bases
ATGAATCTTGAAGTGAAAAAGATTGACACCGCTAACGCCCGTTTGAGCGCTAAACTTTCCATTGAGAATTTAGAAAAGCG
TTATGATAAAATCGCTCAAAAAATCGCCCAAAAAGTTAAAATTGATGGCTTTAGAAGAGGTAAAGTCCCCCTTAGCTTAG
TGAAAACCCGTTATCAAGCCCAAATTGAACAAGACGCTCAAGAAGAAATGATTCAAGAGGTTTTGAAAAACGCTTTTAAG
GAATTAGGGATTGAAAATAAGGATCTCATCGGCAGCCCCAATCTCACTAAATTTGAAAAAAAAGACACGCATTTTGAAAT
AGAAGCGGACATCGGCTTAAAACCCACGATTGTTTTAGACAAGATCAAAGAGTGCGTGCCTAGCGTGGGAGTGGAAGTTC
CAAATGAAGAAAAAATTGATGAGCGTTTGAAACAGCTCGCTAAAGATTATGCGAAATTTGTGGATACCAACACTCAAAGA
AAAGCTCAAAATGACGATAAATTAACGATTGATTTTGAAGGCTTTATAGATAATGCGCCTTTTGAAGGGGGCAAGGCTGA
GAATTTCAATTTGATTTTAGGCAGTAAGCAAATGCTAGAAGATTTTGAAAAGGCTCTTTTAGGCATGCAAGCGGGTGAAG
AAAAAGAATTCCCTTTGACTTTCCCTAGCAAATACCACGCAGAGCATTTAGCCGGCAAAGAAGCTTTTTTTAAAGTGAAA
TTACACCAGATTCAAGCGCGTGAAATGTTAGAAATCAATGACGAACTCGCTAAAATCGTGCTAGCTAATGAAGAGAATGC
GACTTTAAAGCTTTTAAAAGAAAGGGTTGAGGGGCAGTTGTTTTTAGAAAATAAAGCCAGGCTCTATAATGAAGAGTTGA
AAGAAAAATTGATTGAAAATTTAGATGAAAAGATTGTTTTTGATTTGCCTAAAACGATCATAGAGCAAGAAATGGATTTG
TTGTTCAGGAACGCTCTTTATTCCATGCAAGCTGAGGAAGTCAAATCCTTACAAGAAAGTCAAGAAAAAGCCAAAGAAAA
GCGTGAGAGCTTTAGGAATGATGCGACAAAAAGCGTGAAAATCACTTTTATCATTGACGCTTTAGCGAAGGAAGAAAAAA
TTGGCGTGCATGACAATGAAGTCTTTCAAACTTTGTATTATGAAGCGATGATGACAGGGCAAAACCCAGAAAATCTCATT
GAGCAATACCGCAAAAATAACATGTTAGCGGCGGTGAAAATGGCGATGATTGAAGATAGGGTGTTAGCTTATTTGTTGGA
TAAAAACCTGCCTAAAGAGCAACAAGAAATTTTAGAGAAAATGAGACCCAACGCTCAAAAAATTCAAGCGGGTTAA

Upstream 100 bases:

>100_bases
TTTGAAAACTAGAATTAAAACCGCTTTTTATAAACTGGAATTAAAACAAATTAAGCTATAATGATACAAAAATTTAATTT
TTTAGCCATTAAGGAAAAAA

Downstream 100 bases:

>100_bases
ACGGCTAAAAAGGAGAGATGATGGGATACATTCCTTATGTAATAGAGAATACCGATCGTGGGGAGCGTAGCTATGATATT
TACTCGCGCCTTTTAAAGGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNLEVKKIDTANARLSAKLSIENLEKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQAQIEQDAQEEMIQEVLKNAFK
ELGIENKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLDKIKECVPSVGVEVPNEEKIDERLKQLAKDYAKFVDTNTQR
KAQNDDKLTIDFEGFIDNAPFEGGKAENFNLILGSKQMLEDFEKALLGMQAGEEKEFPLTFPSKYHAEHLAGKEAFFKVK
LHQIQAREMLEINDELAKIVLANEENATLKLLKERVEGQLFLENKARLYNEELKEKLIENLDEKIVFDLPKTIIEQEMDL
LFRNALYSMQAEEVKSLQESQEKAKEKRESFRNDATKSVKITFIIDALAKEEKIGVHDNEVFQTLYYEAMMTGQNPENLI
EQYRKNNMLAAVKMAMIEDRVLAYLLDKNLPKEQQEILEKMRPNAQKIQAG

Sequences:

>Translated_451_residues
MNLEVKKIDTANARLSAKLSIENLEKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQAQIEQDAQEEMIQEVLKNAFK
ELGIENKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLDKIKECVPSVGVEVPNEEKIDERLKQLAKDYAKFVDTNTQR
KAQNDDKLTIDFEGFIDNAPFEGGKAENFNLILGSKQMLEDFEKALLGMQAGEEKEFPLTFPSKYHAEHLAGKEAFFKVK
LHQIQAREMLEINDELAKIVLANEENATLKLLKERVEGQLFLENKARLYNEELKEKLIENLDEKIVFDLPKTIIEQEMDL
LFRNALYSMQAEEVKSLQESQEKAKEKRESFRNDATKSVKITFIIDALAKEEKIGVHDNEVFQTLYYEAMMTGQNPENLI
EQYRKNNMLAAVKMAMIEDRVLAYLLDKNLPKEQQEILEKMRPNAQKIQAG
>Mature_451_residues
MNLEVKKIDTANARLSAKLSIENLEKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQAQIEQDAQEEMIQEVLKNAFK
ELGIENKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLDKIKECVPSVGVEVPNEEKIDERLKQLAKDYAKFVDTNTQR
KAQNDDKLTIDFEGFIDNAPFEGGKAENFNLILGSKQMLEDFEKALLGMQAGEEKEFPLTFPSKYHAEHLAGKEAFFKVK
LHQIQAREMLEINDELAKIVLANEENATLKLLKERVEGQLFLENKARLYNEELKEKLIENLDEKIVFDLPKTIIEQEMDL
LFRNALYSMQAEEVKSLQESQEKAKEKRESFRNDATKSVKITFIIDALAKEEKIGVHDNEVFQTLYYEAMMTGQNPENLI
EQYRKNNMLAAVKMAMIEDRVLAYLLDKNLPKEQQEILEKMRPNAQKIQAG

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=416, Percent_Identity=28.6057692307692, Blast_Score=152, Evalue=6e-38,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 51989; Mature: 51989

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEVKKIDTANARLSAKLSIENLEKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQA
CCCEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QIEQDAQEEMIQEVLKNAFKELGIENKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
KIKECVPSVGVEVPNEEKIDERLKQLAKDYAKFVDTNTQRKAQNDDKLTIDFEGFIDNAP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCCCC
FEGGKAENFNLILGSKQMLEDFEKALLGMQAGEEKEFPLTFPSKYHAEHLAGKEAFFKVK
CCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
LHQIQAREMLEINDELAKIVLANEENATLKLLKERVEGQLFLENKARLYNEELKEKLIEN
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHH
LDEKIVFDLPKTIIEQEMDLLFRNALYSMQAEEVKSLQESQEKAKEKRESFRNDATKSVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ITFIIDALAKEEKIGVHDNEVFQTLYYEAMMTGQNPENLIEQYRKNNMLAAVKMAMIEDR
EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
VLAYLLDKNLPKEQQEILEKMRPNAQKIQAG
HHHHHHHCCCCHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MNLEVKKIDTANARLSAKLSIENLEKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQA
CCCEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QIEQDAQEEMIQEVLKNAFKELGIENKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
KIKECVPSVGVEVPNEEKIDERLKQLAKDYAKFVDTNTQRKAQNDDKLTIDFEGFIDNAP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCCCC
FEGGKAENFNLILGSKQMLEDFEKALLGMQAGEEKEFPLTFPSKYHAEHLAGKEAFFKVK
CCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
LHQIQAREMLEINDELAKIVLANEENATLKLLKERVEGQLFLENKARLYNEELKEKLIEN
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHH
LDEKIVFDLPKTIIEQEMDLLFRNALYSMQAEEVKSLQESQEKAKEKRESFRNDATKSVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ITFIIDALAKEEKIGVHDNEVFQTLYYEAMMTGQNPENLIEQYRKNNMLAAVKMAMIEDR
EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
VLAYLLDKNLPKEQQEILEKMRPNAQKIQAG
HHHHHHHCCCCHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA