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Identifier: 15645347

GI number: 15645347

Start: 782071

End: 783057

Strand: Reverse

Name: Not Available

Synonym: HP0727

Alternate gene names: 15645347

Gene position: 783057-782071 (Counterclockwise)

Preceding gene: 15645348

Following gene: 15645346

Centisome position: 46.95

GC content: 40.02

Gene sequence:

>987_bases
ATGGACTTTAAAAATAAAAAATGGCTTTTTCTAGCCCCTTTAGCAGGCTATACGGATTTGCCTTTCAGGAGCGTGGTGAA
AAAATTTGGCGTGGATGTTACCACGAGCGAAATGGTGAGCTCGCATTCGTTGGTGTATGCGTTTGATAAAACTTCTAAAA
TGTTGGAAAAATCCCCTTTAGAAGATCATTTCATGGCGCAAATTTCAGGCTCTAAAGAAAGCGTAGTCAAAGAAGCGGTG
GAGAAAATCAACGCTTTAGAGCATGTGAATGGGATTGATTTTAATTGCGGTTGTCCCGCTCCTAAAGTGGCTAATCATGG
TAATGGTAGTGGGTTATTGAAGGATTTAAACCACTTAGTGAAGCTTTTAAAAACCATCAGAGAAAACACTAGTAAAAAAA
TCACAAGCGTGAAAGTGCGTTTAGGCTTTGAAAAGAAAATCCCTAAAGAAATCGCTCATGCCCTAAATGACGCACCGGTG
GATTATGTGGTGGTGCATGGGAGGACACGAAGCGATAAATACCAAAAAGACAAAATAGATTACGAAAGCATCGCTTTAAT
GAAAAAGATTTTAAAAAAGCCGGTGATAGCCAATGGCGAAATTGACAGCGTGAAAAAGGCTTTTGAAGTTTTACAAATCA
CTCAAGCGGATGGGCTAATGATAGGGCGAGCGGCCTTAAGAGCCCCATGGATATTTTGGCAAATCAGAAACAACACCACA
AAATTACCCGCAGTCGTGAAAAAAGACCTGGTTTTAGAACATTTTGATAAAATGGTGGAGTTTTATGGGGATATGGGGGT
AATCATGTTTAGGAAAAATTTGCATGCTTACGCTAAGGGCGAAATGCAAGCGAGCGCGTTTCGTAACTGCGTCAATACCC
TTACAGAAATAAAGAGCATGCGAGAGAGCATAGAGGAATTTTTTAATCAAGAAATGTTGCAAAGTGAAGTGCCGTTATGG
GTAGAATTGAATCAAAAAAGCGTTTGA

Upstream 100 bases:

>100_bases
TCTATAAAAGAGCTTCATTTTAATCAAGCGATAAGTTTTTAAAGCGCTTTTTAACCTTGATTGTGTCAAAATACGGCAAA
AAACTTTGATATGAGAATAA

Downstream 100 bases:

>100_bases
AAGCGCTTGTTTTTTTAGCCAGCTTGGGGGTTTTGTGGGGCAATAGCGCTGAAAAAACGCCTTTTTTTAAAACGAAAAAC
CACATTTATCTAGGTTTTAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSPLEDHFMAQISGSKESVVKEAV
EKINALEHVNGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKTIRENTSKKITSVKVRLGFEKKIPKEIAHALNDAPV
DYVVVHGRTRSDKYQKDKIDYESIALMKKILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
KLPAVVKKDLVLEHFDKMVEFYGDMGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQSEVPLW
VELNQKSV

Sequences:

>Translated_328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSPLEDHFMAQISGSKESVVKEAV
EKINALEHVNGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKTIRENTSKKITSVKVRLGFEKKIPKEIAHALNDAPV
DYVVVHGRTRSDKYQKDKIDYESIALMKKILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
KLPAVVKKDLVLEHFDKMVEFYGDMGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQSEVPLW
VELNQKSV
>Mature_328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSPLEDHFMAQISGSKESVVKEAV
EKINALEHVNGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKTIRENTSKKITSVKVRLGFEKKIPKEIAHALNDAPV
DYVVVHGRTRSDKYQKDKIDYESIALMKKILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
KLPAVVKKDLVLEHFDKMVEFYGDMGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQSEVPLW
VELNQKSV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=243, Percent_Identity=30.4526748971193, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788462, Length=243, Percent_Identity=30.4526748971193, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=28.125, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=26.2948207171315, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789660, Length=315, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788462, Length=270, Percent_Identity=31.4814814814815, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=275, Percent_Identity=26.9090909090909, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=27.5229357798165, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=251, Percent_Identity=27.8884462151394, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=27.6315789473684, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=25.2173913043478, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=264, Percent_Identity=25, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=257, Percent_Identity=23.7354085603113, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585320, Length=260, Percent_Identity=29.6153846153846, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=28.4444444444444, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24580595, Length=245, Percent_Identity=26.1224489795918, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920448, Length=245, Percent_Identity=26.1224489795918, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37151; Mature: 37151

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00435 PEROXIDASE_1 ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSPL
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECHHHHHHHHCCC
EDHFMAQISGSKESVVKEAVEKINALEHVNGIDFNCGCPAPKVANHGNGSGLLKDLNHLV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLLKTIRENTSKKITSVKVRLGFEKKIPKEIAHALNDAPVDYVVVHGRTRSDKYQKDKID
HHHHHHHHHHHHHHEEEEEEECCHHCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCC
YESIALMKKILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCEEEEEECCCCC
KLPAVVKKDLVLEHFDKMVEFYGDMGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSM
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RESIEEFFNQEMLQSEVPLWVELNQKSV
HHHHHHHHHHHHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSPL
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECHHHHHHHHCCC
EDHFMAQISGSKESVVKEAVEKINALEHVNGIDFNCGCPAPKVANHGNGSGLLKDLNHLV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLLKTIRENTSKKITSVKVRLGFEKKIPKEIAHALNDAPVDYVVVHGRTRSDKYQKDKID
HHHHHHHHHHHHHHEEEEEEECCHHCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCC
YESIALMKKILKKPVIANGEIDSVKKAFEVLQITQADGLMIGRAALRAPWIFWQIRNNTT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCEEEEEECCCCC
KLPAVVKKDLVLEHFDKMVEFYGDMGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSM
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RESIEEFFNQEMLQSEVPLWVELNQKSV
HHHHHHHHHHHHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]