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Identifier: 15645300

GI number: 15645300

Start: 726648

End: 727154

Strand: Reverse

Name: Not Available

Synonym: HP0676

Alternate gene names: 15645300

Gene position: 727154-726648 (Counterclockwise)

Preceding gene: 15645301

Following gene: 15645277

Centisome position: 43.6

GC content: 37.08

Gene sequence:

>507_bases
ATGGTTTTATACCACTACTATTTTAAGACCCCTAAGAGTTTCCCTTTAGAGTATTTGCATTTGTGCGCTAATGAGAGCCA
TTTATTGAGATTGGATTTTGATGCGGCCAATTTTTCTCATAACACCCCTATGAATACCCCATTAAAACTCAGCGTTCAAG
CCTTGGAGCGTTATTTCTTAGGACAACTTTTTGAATTTAATACGCCTTTGGATTTGATAGGGACTTTTTTTCAAAAACAA
GTTTGGTCTGCGTTAATGACTATCCCTTATGGTAAAACAAAAAGTTACGATGAAATCGCAAAGCTCATTAATAACCCTAG
ATCTTGCAGAGCTGTTGGCAACGCCAATCGCAATAACCCTATTTCTTTGATCGTGCCTTGTCATAGAGTGGTGCGTAAAA
ATGGCACTTTAGGGGGGTATAATGGGGGCATAGAAGTCAAAAAGTGGCTGTTAGAATTTGAAAGCAAAATTTTAAACCAG
CAAGCTAAAAACTCTTTAATTTCTTAA

Upstream 100 bases:

>100_bases
GATCATGGGGCTTTTGAATGAAAAAATGCATAAAGCTTTGATTTTAGGGGTGTATGGTTTGTCGCTATTGATTGTTTTAT
ACAAACTCTTTTTTTAATTG

Downstream 100 bases:

>100_bases
AAAATGGGGTTTTGGTTGTTTTATGGTAAAAATTATTTTCTTAAGGGAGTAGGGGGTGTTTTTAATTTTCTTCCCAAATG
CTCGCCGCTTCTTCTAAACG

Product: methylated-DNA--protein-cysteine methyltransferase (dat1)

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MVLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDAANFSHNTPMNTPLKLSVQALERYFLGQLFEFNTPLDLIGTFFQKQ
VWSALMTIPYGKTKSYDEIAKLINNPRSCRAVGNANRNNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQ
QAKNSLIS

Sequences:

>Translated_168_residues
MVLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDAANFSHNTPMNTPLKLSVQALERYFLGQLFEFNTPLDLIGTFFQKQ
VWSALMTIPYGKTKSYDEIAKLINNPRSCRAVGNANRNNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQ
QAKNSLIS
>Mature_168_residues
MVLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDAANFSHNTPMNTPLKLSVQALERYFLGQLFEFNTPLDLIGTFFQKQ
VWSALMTIPYGKTKSYDEIAKLINNPRSCRAVGNANRNNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQ
QAKNSLIS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=86, Percent_Identity=41.8604651162791, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1787596, Length=133, Percent_Identity=42.1052631578947, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1788542, Length=116, Percent_Identity=44.8275862068966, Blast_Score=104, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=47.6190476190476, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=47.6190476190476, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=103, Percent_Identity=39.8058252427184, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19256; Mature: 19256

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDAANFSHNTPMNTPLKLSVQALERYFL
CEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
GQLFEFNTPLDLIGTFFQKQVWSALMTIPYGKTKSYDEIAKLINNPRSCRAVGNANRNNP
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCC
ISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQQAKNSLIS
EEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDAANFSHNTPMNTPLKLSVQALERYFL
CEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
GQLFEFNTPLDLIGTFFQKQVWSALMTIPYGKTKSYDEIAKLINNPRSCRAVGNANRNNP
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCC
ISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQQAKNSLIS
EEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]