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The map label for this gene is ligA

Identifier: 15645240

GI number: 15645240

Start: 659069

End: 661039

Strand: Reverse

Name: ligA

Synonym: HP0615

Alternate gene names: 15645240

Gene position: 661039-659069 (Counterclockwise)

Preceding gene: 15645245

Following gene: 15645239

Centisome position: 39.63

GC content: 39.57

Gene sequence:

>1971_bases
ATGATAAAAAGCCAAAAAGAATATTTAGAAAGAATTGCATATTTAAACACCCTATCGCACCATTATTACAACCTTGATGA
ACCCATCGTAAGCGATGCGATCTATGATGAACTTTACCAAGAATTGAAAGCTTATGAAGAAAAAAACCCTAATGGCATTC
AAGCTAATTCCCCTACCCAAAAAGTGGGGGCTACTACCACCAATTCGTTCAATAAAAACCCCCATTTAATGCGGATGTGG
AGCTTAGATGATGTGTTCAATCAAAGCGAATTGCAAGCGTGGTTGCAACGCATTTTAAAAGCCTATCCTAGTGCTTCGTT
CGTGTGTTCGCCCAAACTTGATGGGGTTTCGCTCAATCTTTTGTATCAACATGGCAAGCTAGTGAAGGCGACCACTAGGG
GCAACGGCTTAGAAGGAGAATTAGTTAGCGCAAACGCTAAACACATCGCTAATATCCCCCACGCTATCGCTTATAATGGA
GAAATAGAAATCAGGGGCGAAGTGATCATTTCTAAAAAGGATTTTGACGCTTTGAATCAAGAGCGCTTAAACGCTAATGA
ACCCCTATTCGCTAACCCCAGAAACGCCGCATCAGGGAGTTTGAGGCAACTTGATAGCGAAATCACTAAAAAGCGTAAAT
TGCAATTCATTCCTTGGGGCGTGGGCAAGCATTCTTTAAATTTTTTAAGCTTTAAGGAGTGTTTGGATTTTATCGTCTCG
TTAGGTTTTAGCGCCATTCAATACTTAAGCCTAAACAAAAACCACCAAGAAATAGAAGACAATTACCACACCCTAATTAG
AGAAAGGGAGGGCTTTTTTGCCCTTTTAGACGGCATGGTGATCGTTGTGAATGAATTAAATATTCAAAAGGAGCTAGGCT
ACACGCAAAAATCCCCTAAATTCGCTTGCGCTTATAAATTCCCGGCTTTAGAAAAACACACCAAAATTGTAGGAGTCATT
AACCAAGTGGGGCGCAGCGGGGCGATCACACCGGTCGCTCTTTTAGAGCCTGTGGAAATTGCTGGAGCTATGATTAATAG
AGCGACCTTACACAATTATTCTGAAATTGAAAAAAAGAATATCATGCTCAGTGATAGGGTCGTTGTCATTAGAAGCGGCG
ATGTGATCCCTAAAATCATCAAGCCTTTAGAATCTTATAGAGACGGCTCGCAACATAAAATTGAACGCCCCAAGGTTTGC
CCTATATGTTCGCATGAGCTTTTGTGCGAAGAGATTTTTACTTATTGTCAAAACCTTAATTGCCCGGCAAGGTTGAAAGA
AAGCTTGATTCATTTCGCTTCTAAAGACGCTTTAAACATTCAAGGCTTGGGCGATAAAGTCATAGAGCAACTTTTTGAAG
AAAAGCTCATTTTTAACGCTCTGGATTTGTATGCTTTAAAATTAGAAGATTTAATGCGGCTAGACAAATTTAAAATTAAA
AAAGCTCAAAATCTATTAGACGCTATTTTAAAAAGCAAAAACCCTCCCTTATGGCGTTTGATTAACGCTTTAGGGATTGA
GCATATTGGTAAGGGAGCGAGTAAAACGCTGGCCAAATACGGCTTAAATGTGTTAGAAAAAAGCGAAGCCGAGTTTTTAG
AAATGGAAGGCTTTGGGGTGGAAATGGCGCGCTCTTTAGTCAATTTTTATGCGAGCAATCAAGAATTTATCCGATCGTTA
TTTGAGTTATTAAACCCTAAAAATAGCGATATGGCTGAAGAAAAGCAAAAAAGCTCTTCTGTTTTCAATAATAAAACGAT
TGTTTTAACCGGCACGCTTTCTAAACCACGGCAAGAATACGCTCAAATGTTAGAAAATTTAGGGGCAAAAATTTCTTCAA
GCGTGAGCGCTAAAACCGATTTTTTAATCGCTGGAGAAAACCCCGGCTCAAAACTCGCTCTGGCACAAAAACATGGCGTG
AGCGTTTTGAATGAAGAAGAATTATTAAAGCGCCTTAAAGAATTGGATTAA

Upstream 100 bases:

>100_bases
GTTTTGTCAATATCTCTTACCACCTATTTTCCCCTAAAGCCCTATCAAAGATTAAATGCTATAATACTACCCTAATTTAG
TTTAAAAGGTTTTTACAAAC

Downstream 100 bases:

>100_bases
AATTAAATTCTAAAGAAAAATTCCAATTTTCATTCTTAATGAATTTACTCACAAGATCCTATTTTATAGTAGAATGCGAA
AAATAAAGATACTTTAAGGA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MIKSQKEYLERIAYLNTLSHHYYNLDEPIVSDAIYDELYQELKAYEEKNPNGIQANSPTQKVGATTTNSFNKNPHLMRMW
SLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNG
EIEIRGEVIISKKDFDALNQERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
LGFSAIQYLSLNKNHQEIEDNYHTLIREREGFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGVI
NQVGRSGAITPVALLEPVEIAGAMINRATLHNYSEIEKKNIMLSDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVC
PICSHELLCEEIFTYCQNLNCPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAILKSKNPPLWRLINALGIEHIGKGASKTLAKYGLNVLEKSEAEFLEMEGFGVEMARSLVNFYASNQEFIRSL
FELLNPKNSDMAEEKQKSSSVFNNKTIVLTGTLSKPRQEYAQMLENLGAKISSSVSAKTDFLIAGENPGSKLALAQKHGV
SVLNEEELLKRLKELD

Sequences:

>Translated_656_residues
MIKSQKEYLERIAYLNTLSHHYYNLDEPIVSDAIYDELYQELKAYEEKNPNGIQANSPTQKVGATTTNSFNKNPHLMRMW
SLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNG
EIEIRGEVIISKKDFDALNQERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
LGFSAIQYLSLNKNHQEIEDNYHTLIREREGFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGVI
NQVGRSGAITPVALLEPVEIAGAMINRATLHNYSEIEKKNIMLSDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVC
PICSHELLCEEIFTYCQNLNCPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAILKSKNPPLWRLINALGIEHIGKGASKTLAKYGLNVLEKSEAEFLEMEGFGVEMARSLVNFYASNQEFIRSL
FELLNPKNSDMAEEKQKSSSVFNNKTIVLTGTLSKPRQEYAQMLENLGAKISSSVSAKTDFLIAGENPGSKLALAQKHGV
SVLNEEELLKRLKELD
>Mature_656_residues
MIKSQKEYLERIAYLNTLSHHYYNLDEPIVSDAIYDELYQELKAYEEKNPNGIQANSPTQKVGATTTNSFNKNPHLMRMW
SLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNG
EIEIRGEVIISKKDFDALNQERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
LGFSAIQYLSLNKNHQEIEDNYHTLIREREGFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGVI
NQVGRSGAITPVALLEPVEIAGAMINRATLHNYSEIEKKNIMLSDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVC
PICSHELLCEEIFTYCQNLNCPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAILKSKNPPLWRLINALGIEHIGKGASKTLAKYGLNVLEKSEAEFLEMEGFGVEMARSLVNFYASNQEFIRSL
FELLNPKNSDMAEEKQKSSSVFNNKTIVLTGTLSKPRQEYAQMLENLGAKISSSVSAKTDFLIAGENPGSKLALAQKHGV
SVLNEEELLKRLKELD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=665, Percent_Identity=38.3458646616541, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI87082305, Length=444, Percent_Identity=22.0720720720721, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73901; Mature: 73901

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSQKEYLERIAYLNTLSHHYYNLDEPIVSDAIYDELYQELKAYEEKNPNGIQANSPTQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
KVGATTTNSFNKNPHLMRMWSLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNL
HCCCCCCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
LYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNGEIEIRGEVIISKKDFDALNQ
EEECCCEEEEECCCCCCCCCEECCCCHHHHCCCCEEEECCEEEEEEEEEEECCCHHHHHH
ERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHCHHHHHHHHHHHHHH
LGFSAIQYLSLNKNHQEIEDNYHTLIREREGFFALLDGMVIVVNELNIQKELGYTQKSPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEEECCCHHHHCCCCCCCCC
FACAYKFPALEKHTKIVGVINQVGRSGAITPVALLEPVEIAGAMINRATLHNYSEIEKKN
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHCCHHHHHHHC
IMLSDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVCPICSHELLCEEIFTYCQNLN
EEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
CPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQNLLDAILKSKNPPLWRLINALGIEHIGKGASKTLAKYGLNVLEKSEAEFLEMEGFGV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHCCHHHHHHCCCCH
EMARSLVNFYASNQEFIRSLFELLNPKNSDMAEEKQKSSSVFNNKTIVLTGTLSKPRQEY
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AQMLENLGAKISSSVSAKTDFLIAGENPGSKLALAQKHGVSVLNEEELLKRLKELD
HHHHHHHCCHHHCCCCCCCCEEEECCCCCCCEEEEHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIKSQKEYLERIAYLNTLSHHYYNLDEPIVSDAIYDELYQELKAYEEKNPNGIQANSPTQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
KVGATTTNSFNKNPHLMRMWSLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNL
HCCCCCCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
LYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNGEIEIRGEVIISKKDFDALNQ
EEECCCEEEEECCCCCCCCCEECCCCHHHHCCCCEEEECCEEEEEEEEEEECCCHHHHHH
ERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHCHHHHHHHHHHHHHH
LGFSAIQYLSLNKNHQEIEDNYHTLIREREGFFALLDGMVIVVNELNIQKELGYTQKSPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEEECCCHHHHCCCCCCCCC
FACAYKFPALEKHTKIVGVINQVGRSGAITPVALLEPVEIAGAMINRATLHNYSEIEKKN
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHCCHHHHHHHC
IMLSDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVCPICSHELLCEEIFTYCQNLN
EEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
CPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQNLLDAILKSKNPPLWRLINALGIEHIGKGASKTLAKYGLNVLEKSEAEFLEMEGFGV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHCCHHHHHHCCCCH
EMARSLVNFYASNQEFIRSLFELLNPKNSDMAEEKQKSSSVFNNKTIVLTGTLSKPRQEY
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AQMLENLGAKISSSVSAKTDFLIAGENPGSKLALAQKHGVSVLNEEELLKRLKELD
HHHHHHHCCHHHCCCCCCCCEEEECCCCCCCEEEEHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA