Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 15645191

GI number: 15645191

Start: 598047

End: 598868

Strand: Reverse

Name: dapF

Synonym: HP0566

Alternate gene names: 15645191

Gene position: 598868-598047 (Counterclockwise)

Preceding gene: 15645193

Following gene: 15645190

Centisome position: 35.91

GC content: 39.17

Gene sequence:

>822_bases
ATGGTGTTTTACAAGTATTCAGGGAGCGGGAATGATTTTTTAATCGTTCAAAGTTTCAAAAAAAAAGATTTTTCAAATTT
AGCCAAACAGGTGTGCCATAGGCATGAGGGTTTTGGGGCTGATGGGCTTGTAGTCGTCTTACCGAGTAAGGATTATGACT
ACGAATGGGATTTTTACAATTCAGACGGCTCTAAAGCTGGCATGTGCGGGAATGCGAGCCGTTGCGTGGGGTTATTTGCT
TACCAGCATGCTATAGCCTCTAAAAACCATGTTTTTTTAGCCGGAAAAAGAGAGATTTCTATTTGCATAGAAGAACCCAA
TATCATAGAGAGCAATCTTGGTAACTACAAAATCCTAGATGTAATACCCGCTTTAAGATGCGAAAAATTTTTTTCAAGCG
GCAGCGTTTTAGAACATATCCCTACTTTCTATCTCATAGATACAGGAGTGCCGCATTTAGTGGGATTTGTGGAAAATAAA
GAGGGGTTAAATTCCCTTAACACGCTAGAATTAAGGGCTTTAAGGCATGAATTTAACGCTAATATTAACATCGCTTTTAT
AGAAAATAAAGAGACGATTTTTTTACAAACTTATGAAAGAGGGGTTGAAGATTTCACGCTGGCTTGCGGGACAGGCATGG
CGGCGGTTTTTATCGCCGCACGCATTTTTTATAACACGCCTAAAAAAGCCGCTCTCATCCCTAAAAGCAACGAATCTTTA
GAGCTTTCTTTAAAAAATGATGGAATTTTTTATAAAGGCGCGGTGCGTTATATTGGCATGAGTGTTTTGGGCATGAGGGT
TTTTGAAAATGGGTGTTTTTGA

Upstream 100 bases:

>100_bases
AAACTGAACCAAAAATTATCAAATGTTAGCTAAAAAACGCTCTTTTAAGGATAAAAAACCTCTTTTAACTTATAATTAAA
ATCAAAAAGGCTTATCATAG

Downstream 100 bases:

>100_bases
TCATTATTTCTTGTAATAGTAATGATTTTGTTGCAATTAAACATAATGCGCTATCATTTCTAGGCTGTTTTTAGCAATAT
AAAAAATATCCTAAAGGAAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILDVIPALRCEKFFSSGSVLEHIPTFYLIDTGVPHLVGFVENK
EGLNSLNTLELRALRHEFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF

Sequences:

>Translated_273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILDVIPALRCEKFFSSGSVLEHIPTFYLIDTGVPHLVGFVENK
EGLNSLNTLELRALRHEFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF
>Mature_273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILDVIPALRCEKFFSSGSVLEHIPTFYLIDTGVPHLVGFVENK
EGLNSLNTLELRALRHEFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=226, Percent_Identity=29.2035398230088, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30500; Mature: 30500

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYN
CEEEEEECCCCEEEEEECHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECEEEC
SDGSKAGMCGNASRCVGLFAYQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCHHCCCCCEEEEH
VIPALRCEKFFSSGSVLEHIPTFYLIDTGVPHLVGFVENKEGLNSLNTLELRALRHEFNA
HHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHEEEECCCCCCCCCCHHHHHHHHHHHCC
NINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
CEEEEEEECCCEEEEEEHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCE
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF
EEEECCCCEEEECHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYN
CEEEEEECCCCEEEEEECHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECEEEC
SDGSKAGMCGNASRCVGLFAYQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCHHCCCCCEEEEH
VIPALRCEKFFSSGSVLEHIPTFYLIDTGVPHLVGFVENKEGLNSLNTLELRALRHEFNA
HHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHEEEECCCCCCCCCCHHHHHHHHHHHCC
NINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
CEEEEEEECCCEEEEEEHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCE
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF
EEEECCCCEEEECHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA