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Identifier: 15645174

GI number: 15645174

Start: 584583

End: 585350

Strand: Reverse

Name: Not Available

Synonym: HP0549

Alternate gene names: 15645174

Gene position: 585350-584583 (Counterclockwise)

Preceding gene: 15645175

Following gene: 15645172

Centisome position: 35.1

GC content: 41.54

Gene sequence:

>768_bases
ATGAAAATAGGCGTTTTTGATAGCGGTGTGGGAGGGTTTAGCGTTTTAAAAAGCCTTTTAAAAGCGCAACTATTTGATGA
AATCATCTATTATGGCGATAGCGCTAGAGTGCCTTATGGCACTAAAGACCCCACCACGATCAAGCAATTTGGCTTAGAGG
CTTTGGATTTTTTCAAACCGCACCAGATTAAATTATTGATTGTGGCATGCAACACCGCAAGCGCTCTGGCTTTAGAAGAG
ATGCAAAAGCATTCCAAAATCCCTGTTGTGGGCGTGATTGAGCCAAGCATTTTAGCGATCAAACGGCAAGTGAAAGATAA
AAACGCCCCTATTTTGGTGCTAGGGACAAAAGCGACGATTCAATCCAACGCCTATGATAACGCCCTGAAACAACAAGGCT
ATTTGAATGTTTCGCATTTAGCCACTTCTCTTTTTGTGCCTTTGATTGAAGAAAGTATTTTAGAGGGCGAATTGCTAGAA
ACTTGCATGCGTTATTATTTCACTCCATTAGAGATTTTACCTGAAGTGGTTATTTTAGGTTGCACGCATTTTCCCTTGAT
CGCTCAAAAAATTGAGGGCTATTTTATGGAGCATTTTGCCCTTTCAACGCCCCCCCTACTCATCCATTCTGGCGATGCTA
TTGTGGAATATTTGCAACAAAATTACGCCCTTAAGAAAAACGCATGCGCGTTCCCTAAAGTGGAATTTCATGCGAGCGGC
GATGTGGTTTGGCTAGAAAAACAAGCTAAAGAATGGCTTAAATTGTAA

Upstream 100 bases:

>100_bases
TATTCTAAAATGCAACAAACTAAGGACAATGAAGAATTTTTAAATTTAATGAATGAAAAATAAAATCCCTAATTAATCCC
ATTAAATTAAAAATAGTGTC

Downstream 100 bases:

>100_bases
CGCTAATAAAATAACTCAATTCCAAAAAGGGGCTATAGCTCACATTATAAAGGGTATTGTGTTGATTATAAAAAGCACTT
TAGAGAGATTTACGAAAGTG

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKPHQIKLLIVACNTASALALEE
MQKHSKIPVVGVIEPSILAIKRQVKDKNAPILVLGTKATIQSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLE
TCMRYYFTPLEILPEVVILGCTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQNYALKKNACAFPKVEFHASG
DVVWLEKQAKEWLKL

Sequences:

>Translated_255_residues
MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKPHQIKLLIVACNTASALALEE
MQKHSKIPVVGVIEPSILAIKRQVKDKNAPILVLGTKATIQSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLE
TCMRYYFTPLEILPEVVILGCTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQNYALKKNACAFPKVEFHASG
DVVWLEKQAKEWLKL
>Mature_255_residues
MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKPHQIKLLIVACNTASALALEE
MQKHSKIPVVGVIEPSILAIKRQVKDKNAPILVLGTKATIQSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLE
TCMRYYFTPLEILPEVVILGCTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQNYALKKNACAFPKVEFHASG
DVVWLEKQAKEWLKL

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=188, Percent_Identity=30.8510638297872, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 28414; Mature: 28414

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKP
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCHHHHHHCCC
HQIKLLIVACNTASALALEEMQKHSKIPVVGVIEPSILAIKRQVKDKNAPILVLGTKATI
CEEEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCEEEEECCHHC
QSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLETCMRYYFTPLEILPEVVILG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHC
CTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQNYALKKNACAFPKVEFHASG
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEECCC
DVVWLEKQAKEWLKL
CEEEEHHHHHHHHCC
>Mature Secondary Structure
MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKP
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCHHHHHHCCC
HQIKLLIVACNTASALALEEMQKHSKIPVVGVIEPSILAIKRQVKDKNAPILVLGTKATI
CEEEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCEEEEECCHHC
QSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLETCMRYYFTPLEILPEVVILG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHC
CTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQNYALKKNACAFPKVEFHASG
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEECCC
DVVWLEKQAKEWLKL
CEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]