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The map label for this gene is gltX

Identifier: 15645104

GI number: 15645104

Start: 497510

End: 498901

Strand: Reverse

Name: gltX

Synonym: HP0476

Alternate gene names: 15645104

Gene position: 498901-497510 (Counterclockwise)

Preceding gene: 15645112

Following gene: 15645100

Centisome position: 29.91

GC content: 39.3

Gene sequence:

>1392_bases
ATGAGTTTGATCGTTACGCGCTTCGCTCCATCGCCCACTGGCTACCTCCACATAGGAGGCTTAAGAACAGCCATTTTCAA
TTATCTTTTTGCACGAGCCAATCAAGGAAAATTTTTTTTACGCATTGAAGACACGGATTTGAGCCGTAACTCTATAGAAG
CGGCTAACGCCATTATAGAAGCTTTCAAATGGGTAGGGCTAGAATACGATGGCGAAATCCTCTACCAATCCAAACGCTTT
GAGATTTATAAAGAATACATTCAAAAACTCTTAGATGAAGACAAAGCCTATTATTGTTACATGAGCAAAGAAGAGTTGGA
CGCTTTGAGAGAAGAGCAAAAAGCCAGGAAAGAAACCCCACGCTATGACAATCGCTATCGTGATTTTAAAGGCACGCCTC
CTAAAGGCATAGAGCCTGTGGTAAGGATTAAAGTCCCCCAAAATGAGGTGATTGGTTTTAATGACGGGGTTAAAGGCGAA
GTGAAAGTGAATACTAACGAATTAGACGATTTTATTATCGCCAGGAGCGATGGGACACCCACTTATAACTTTGTGGTTAC
TATTGATGACGCTTTAATGGGGATTACTGATGTGATTAGAGGCGATGATCACCTTTCTAACACCCCTAAACAAATCGTTC
TTTATAAGGCTTTGAATTTTAAAATCCCTAATTTTTTCCATGTGCCGATGATTTTGAATGAAGAAGGGCAAAAATTAAGC
AAACGCCATGGGGCCACTAATGTGATGGACTATCAAGAAATGGGCTATCTTAAGGAAGCTTTAGTGAATTTTTTAGCGCG
TTTGGGGTGGAGCTATCAGGATAAAGAGGTTTTTAGCATGCAAGAATTGCTAGAATTATTTGATCCTAAAGATTTGAATT
CTTCGCCCAGTTGCTTCAGCTGGCACAAGCTTAATTGGCTCAACGCTCATTATTTAAAAAACCAAAGTGTGCAAGAATTG
TTAAAACTTTTAAAGCCTTTTAGTTTTAGCGATCTCTCGCATTTAAACCCCACTCAATTGGATCGCTTGTTAGACGCTCT
CAAAGAAAGATCTCAAACACTAAAAGAATTAGCCCTTAAAATAGATGAGGTTTTAATCGCCCCTGTGGAGTATGAAGAAA
AGGTTTTTAAAAAACTCAATCAAGCGCTCGTTATGCCCTTGTTAGAAAAGTTTAAGCTAGAATTAAACAAAGCCAATTTC
AACGATGAAAGCGCGCTAGAAAACGCCATGCGCCAAATCATTGAAGAAGAAAAGATTAAAGCGGGTAGTTTTATGCAGCC
TTTAAGATTGGCCCTTTTGGGTAAGGGAGGCGGGATAGGCCTTAAAGAAGCGCTTTTTATTTTAGGCAAAACAGAGAGCG
TCAAAAGAATAGAGGATTTTTTGAAAAACTAA

Upstream 100 bases:

>100_bases
AATAATCTCAATCATACACTAATTTTAATAGAAAAAAGTGAAACCATTTTTAAGCCATTTTTGAATACAATAAGAGTATT
TTATTTTTAAGGTTAGAACA

Downstream 100 bases:

>100_bases
AAAATTGGCTCTGTTTTCATTGGGTATTAAAATAAAAGAGTTTGATTTTAGCGGTTGTTAAGCAACTCCTTTTGAAAAAT
AAGAGGGTTTTAAGAGGAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEYDGEILYQSKRF
EIYKEYIQKLLDEDKAYYCYMSKEELDALREEQKARKETPRYDNRYRDFKGTPPKGIEPVVRIKVPQNEVIGFNDGVKGE
VKVNTNELDDFIIARSDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
KRHGATNVMDYQEMGYLKEALVNFLARLGWSYQDKEVFSMQELLELFDPKDLNSSPSCFSWHKLNWLNAHYLKNQSVQEL
LKLLKPFSFSDLSHLNPTQLDRLLDALKERSQTLKELALKIDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANF
NDESALENAMRQIIEEEKIKAGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEDFLKN

Sequences:

>Translated_463_residues
MSLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEYDGEILYQSKRF
EIYKEYIQKLLDEDKAYYCYMSKEELDALREEQKARKETPRYDNRYRDFKGTPPKGIEPVVRIKVPQNEVIGFNDGVKGE
VKVNTNELDDFIIARSDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
KRHGATNVMDYQEMGYLKEALVNFLARLGWSYQDKEVFSMQELLELFDPKDLNSSPSCFSWHKLNWLNAHYLKNQSVQEL
LKLLKPFSFSDLSHLNPTQLDRLLDALKERSQTLKELALKIDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANF
NDESALENAMRQIIEEEKIKAGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEDFLKN
>Mature_462_residues
SLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEYDGEILYQSKRFE
IYKEYIQKLLDEDKAYYCYMSKEELDALREEQKARKETPRYDNRYRDFKGTPPKGIEPVVRIKVPQNEVIGFNDGVKGEV
KVNTNELDDFIIARSDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLSK
RHGATNVMDYQEMGYLKEALVNFLARLGWSYQDKEVFSMQELLELFDPKDLNSSPSCFSWHKLNWLNAHYLKNQSVQELL
KLLKPFSFSDLSHLNPTQLDRLLDALKERSQTLKELALKIDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANFN
DESALENAMRQIIEEEKIKAGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEDFLKN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.3643724696356, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI62241042, Length=256, Percent_Identity=24.609375, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4826960, Length=287, Percent_Identity=26.1324041811847, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=42.9501084598698, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI226510925, Length=282, Percent_Identity=34.0425531914894, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1786895, Length=213, Percent_Identity=26.2910798122066, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=490, Percent_Identity=33.0612244897959, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17511007, Length=255, Percent_Identity=25.0980392156863, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543332, Length=363, Percent_Identity=24.5179063360882, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=33.469387755102, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=29.3577981651376, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=128, Percent_Identity=30.46875, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=33.5365853658537, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24649466, Length=250, Percent_Identity=26.4, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17864488, Length=289, Percent_Identity=25.9515570934256, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53352; Mature: 53221

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIE
CCEEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
AFKWVGLEYDGEILYQSKRFEIYKEYIQKLLDEDKAYYCYMSKEELDALREEQKARKETP
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDNRYRDFKGTPPKGIEPVVRIKVPQNEVIGFNDGVKGEVKVNTNELDDFIIARSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCEEEEECCCCC
TYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
EEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCCEEECCEEECCCHHHHH
KRHGATNVMDYQEMGYLKEALVNFLARLGWSYQDKEVFSMQELLELFDPKDLNSSPSCFS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
WHKLNWLNAHYLKNQSVQELLKLLKPFSFSDLSHLNPTQLDRLLDALKERSQTLKELALK
ECCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANFNDESALENAMRQIIEEEKIK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEDFLKN
CCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIE
CEEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
AFKWVGLEYDGEILYQSKRFEIYKEYIQKLLDEDKAYYCYMSKEELDALREEQKARKETP
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDNRYRDFKGTPPKGIEPVVRIKVPQNEVIGFNDGVKGEVKVNTNELDDFIIARSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCEEEEECCCCC
TYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
EEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCCEEECCEEECCCHHHHH
KRHGATNVMDYQEMGYLKEALVNFLARLGWSYQDKEVFSMQELLELFDPKDLNSSPSCFS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
WHKLNWLNAHYLKNQSVQELLKLLKPFSFSDLSHLNPTQLDRLLDALKERSQTLKELALK
ECCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANFNDESALENAMRQIIEEEKIK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEDFLKN
CCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA