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Identifier: 15645002

GI number: 15645002

Start: 383091

End: 383771

Strand: Reverse

Name: Not Available

Synonym: HP0374

Alternate gene names: 15645002

Gene position: 383771-383091 (Counterclockwise)

Preceding gene: 15645003

Following gene: 15644999

Centisome position: 23.01

GC content: 40.68

Gene sequence:

>681_bases
ATGCGTTTTGTCTATCACCCTTTAGCCAAAGAGCCTGTTTTAAAAATAGAAGGCGAGAGTTATACGCATTTATACCGATC
AAGGCGTGTCAAAAGTGCGAGTCGTTTGGATTTGAGAAATTTAAAAGACGGCTTTTTATACACCTATGAGCATGCAGAAA
TCACTAAAAAACACGCCCTTTTAAAGCTAGTGGGCGCGCGATTATTAGAGGTTATGGCCAGTAAAAAAACGCATTTGATT
TTAAGCGTGATTGAAATCAAAAACATTGAAAAAATCCTACCCTTTTTAAATCAGTTAGGCGTGAGCAAGTTGAGTTTATT
CTATGCGGATTTTAGCCAACGCAATGAAAAAATAGACATCGCTAAATTAGAGCGCTTTCAAAAGATTTTGATCCATTCTT
GCGAGCAGTGTGGTAGGAGTGCTTTAATGGAATTGGAAGTGTTTTCAAACACTAAAGAGGCGCTAAAAGCCTATCCTAAG
GCGAGCGTTTTGGATTTTAAGGGCGAAACCTTACCCGCAAGCGCGGATTTTGAAAAGGGCGTTATCATAGGGCCTGAGGG
GGGCTTTAGCGAACCAGAAAGAGGGTATTTTAAAGAGCGTGAAATTTATCGCATCCCGTTAGATATGGTGCTAAAGTCTG
AGAGTGCATGCGTGTTTGTAGCGAGTATCGCACAAGTTTAG

Upstream 100 bases:

>100_bases
CGCTATCACTAAAAAAGAAAGGATGGAAGCGTATATCAAAAAGGCTAAAATCCTGTATTTTTTAGAAACGATTCTTATTG
TTGTGTTAATGGGCCTTTGA

Downstream 100 bases:

>100_bases
GGGGTTATTGGGGATTTTAAATCCTAAAAAATCCACCCGTAATTAAAAAACACTTTAAAATGACTGCCCCCTTCATTCAA
AACAACGGAGTTCGCATAGA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRFVYHPLAKEPVLKIEGESYTHLYRSRRVKSASRLDLRNLKDGFLYTYEHAEITKKHALLKLVGARLLEVMASKKTHLI
LSVIEIKNIEKILPFLNQLGVSKLSLFYADFSQRNEKIDIAKLERFQKILIHSCEQCGRSALMELEVFSNTKEALKAYPK
ASVLDFKGETLPASADFEKGVIIGPEGGFSEPERGYFKEREIYRIPLDMVLKSESACVFVASIAQV

Sequences:

>Translated_226_residues
MRFVYHPLAKEPVLKIEGESYTHLYRSRRVKSASRLDLRNLKDGFLYTYEHAEITKKHALLKLVGARLLEVMASKKTHLI
LSVIEIKNIEKILPFLNQLGVSKLSLFYADFSQRNEKIDIAKLERFQKILIHSCEQCGRSALMELEVFSNTKEALKAYPK
ASVLDFKGETLPASADFEKGVIIGPEGGFSEPERGYFKEREIYRIPLDMVLKSESACVFVASIAQV
>Mature_226_residues
MRFVYHPLAKEPVLKIEGESYTHLYRSRRVKSASRLDLRNLKDGFLYTYEHAEITKKHALLKLVGARLLEVMASKKTHLI
LSVIEIKNIEKILPFLNQLGVSKLSLFYADFSQRNEKIDIAKLERFQKILIHSCEQCGRSALMELEVFSNTKEALKAYPK
ASVLDFKGETLPASADFEKGVIIGPEGGFSEPERGYFKEREIYRIPLDMVLKSESACVFVASIAQV

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25771; Mature: 25771

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVYHPLAKEPVLKIEGESYTHLYRSRRVKSASRLDLRNLKDGFLYTYEHAEITKKHAL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
LKLVGARLLEVMASKKTHLILSVIEIKNIEKILPFLNQLGVSKLSLFYADFSQRNEKIDI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCH
AKLERFQKILIHSCEQCGRSALMELEVFSNTKEALKAYPKASVLDFKGETLPASADFEKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCC
VIIGPEGGFSEPERGYFKEREIYRIPLDMVLKSESACVFVASIAQV
EEECCCCCCCCCCCCCCCCCCEEECCHHHHCCCCCCEEEEEEHHCC
>Mature Secondary Structure
MRFVYHPLAKEPVLKIEGESYTHLYRSRRVKSASRLDLRNLKDGFLYTYEHAEITKKHAL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
LKLVGARLLEVMASKKTHLILSVIEIKNIEKILPFLNQLGVSKLSLFYADFSQRNEKIDI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCH
AKLERFQKILIHSCEQCGRSALMELEVFSNTKEALKAYPKASVLDFKGETLPASADFEKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCC
VIIGPEGGFSEPERGYFKEREIYRIPLDMVLKSESACVFVASIAQV
EEECCCCCCCCCCCCCCCCCCEEECCHHHHCCCCCCEEEEEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]