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Identifier: 15644655

GI number: 15644655

Start: 21152

End: 22717

Strand: Reverse

Name: Not Available

Synonym: HP0022

Alternate gene names: 15644655

Gene position: 22717-21152 (Counterclockwise)

Preceding gene: 15644658

Following gene: 15644654

Centisome position: 1.36

GC content: 39.97

Gene sequence:

>1566_bases
TTGGCATCATTATTCCATCTGAGGTTTTTAAAACCCCTAAGTTGTCTGCAAGCCGGTTTGCTTTATAGCCTTATTTTTGG
CGTTTTATACCATTTCCCTTTGTTCGTTTATGTTTATAAAGAAAGCAACCAGGTCAGTTTTATCGCCATGATGGTTGTGG
TGCTTTTTTGCGTTAATGGCGCTCTTTTTTTGGCGTTAGGCTTGATCTCTGCTTCTTTGATGCGTTGGAGTGCGATAGTT
TTTAGCCTGCTCAATTCCGTTGCTTTCTATTTCATTAGCGCTTATAAGGTGTTTTTAAATAAGAGCATGATGGGTAATGT
CTTAAACACCAACACGCATGAAGTTTTAGGCTTTTTGAGCGTCAAATTATTCGTTTTTATCGTTGTTTTTGGGGTGTTGC
CTGGCTATGTCATCTATAAAATCCCCCTTAAAAATTCTTCTAAAAAAGCGCCCTTTTTAGCGATCTTGGCGTTAGTGTTT
ATCTTTATCGCTAGCGCTTTAGCTAACACTAAAAATTGGCTGTGGTTTGACAAGCATGCGAAATTCATAGGGGGCTTAAT
TTTGCCCTTCGCTTATAGCGTGAACGCTTTTAGAGTGAGCGCTCTCAAATTTTTCGCCCCCACCATCAAGCCGCTCCCTC
TTTTTTCACCCAATCATTCCCATTCGTTTGTGGTGCTAGTCATTGGCGAAAGCGCTAGGAAACATAATTACGCCCTTTAT
GGCTATCAAAAACCCACCACCCCAAGACTAAGCAAGCGTTTAGCCGATAATGAACTCACTCTTTTCAACGCCACTTCTTG
CGCCACTTACACGACAGCGAGTTTGGAATGCATTTTAGATTCTTCTTTTAAAAACAACGCTTATGAAAATTTGCCAACTT
ACTTGACTAAAGCCGGTATCAAAGTCTTTTGGTATAGCGCGAACGACGGCGAAAAGAATGTTAAGGTTACAAGCTATCTT
AAAAACTATGAATTGATTCAAAAATGCCCCAATTGTGAAGCGATCGCTCCTTATGATGAATCTTTACTTTATAATTTGCC
TGACCTTTTAAAAGAACACTCTAATGAAAATGTCTTGCTCATCTTACACCTTGCAGGCTCGCATGGCCCAAACTACGACA
ACAAAGTGCCTTTAAATTTTAGGGTGTTTAAGCCTTATTGCTCAAGCGCTGATCTGTCTTCTTGCTCCAAAGAAAGCCTG
ATTAACGCCTATGACAACACCATTTTTTACAACGACTATCTGCTAGATAAGATCATTAGCATGCTTGAAAACGCCAAGCA
GCCCGCCTTAATGATCTATTTAAGCGATCATGGCGAAAGTTTGGGCGAAGAAGCGTTCTATTTGCATGGCATTCCTAAAA
GCATCGCCCCCAAAGAACAATACGAGATCCCCTTTATCGTTTATGCTAATGAGCCTTTCAAAGAAAAGCATTCCATCATT
CAAACCCAAACCCCCATTAATCAAAATGTGATTTTCCATAGCGTTTTAGGGGTGTTTTTGGATTTTAAAAACCCAAGCGT
TGTTTATCGCCCTTCTTTAGATCTGCTTAAACACAAAAAAGAGTAA

Upstream 100 bases:

>100_bases
ATGCAACCACCCTAAAAAAAGCACTTTCCACTTTAAATAAACCATAAAATCCCCATACCTACGATATAATCCTAAGATTG
ATTGATTTTAAGGGGCGTGT

Downstream 100 bases:

>100_bases
AATAACACGCATGAAAAAATTCTTATTTAAACAAAAATTTTGTGAAAGCCTGCCCAAAAGCTTTTCTAAAACTTTGTTAG
CGCTCAGTTTGGGCTTGATT

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MASLFHLRFLKPLSCLQAGLLYSLIFGVLYHFPLFVYVYKESNQVSFIAMMVVVLFCVNGALFLALGLISASLMRWSAIV
FSLLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLSVKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVF
IFIASALANTKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
GYQKPTTPRLSKRLADNELTLFNATSCATYTTASLECILDSSFKNNAYENLPTYLTKAGIKVFWYSANDGEKNVKVTSYL
KNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESL
INAYDNTIFYNDYLLDKIISMLENAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHSII
QTQTPINQNVIFHSVLGVFLDFKNPSVVYRPSLDLLKHKKE

Sequences:

>Translated_521_residues
MASLFHLRFLKPLSCLQAGLLYSLIFGVLYHFPLFVYVYKESNQVSFIAMMVVVLFCVNGALFLALGLISASLMRWSAIV
FSLLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLSVKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVF
IFIASALANTKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
GYQKPTTPRLSKRLADNELTLFNATSCATYTTASLECILDSSFKNNAYENLPTYLTKAGIKVFWYSANDGEKNVKVTSYL
KNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESL
INAYDNTIFYNDYLLDKIISMLENAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHSII
QTQTPINQNVIFHSVLGVFLDFKNPSVVYRPSLDLLKHKKE
>Mature_520_residues
ASLFHLRFLKPLSCLQAGLLYSLIFGVLYHFPLFVYVYKESNQVSFIAMMVVVLFCVNGALFLALGLISASLMRWSAIVF
SLLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLSVKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFI
FIASALANTKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALYG
YQKPTTPRLSKRLADNELTLFNATSCATYTTASLECILDSSFKNNAYENLPTYLTKAGIKVFWYSANDGEKNVKVTSYLK
NYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESLI
NAYDNTIFYNDYLLDKIISMLENAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHSIIQ
TQTPINQNVIFHSVLGVFLDFKNPSVVYRPSLDLLKHKKE

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=488, Percent_Identity=28.4836065573771, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082286, Length=274, Percent_Identity=30.2919708029197, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87082223, Length=254, Percent_Identity=29.5275590551181, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790392, Length=530, Percent_Identity=23.7735849056604, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787035, Length=277, Percent_Identity=25.9927797833935, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 58859; Mature: 58728

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLFHLRFLKPLSCLQAGLLYSLIFGVLYHFPLFVYVYKESNQVSFIAMMVVVLFCVNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHCH
ALFLALGLISASLMRWSAIVFSLLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFIFIASALANTKNWLWFDKHA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHH
KFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GYQKPTTPRLSKRLADNELTLFNATSCATYTTASLECILDSSFKNNAYENLPTYLTKAGI
ECCCCCCHHHHHHHCCCCEEEEECCCCCEEECCEEEEEECCCCCCCCHHHHHHHHHHCCC
KVFWYSANDGEKNVKVTSYLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLL
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHCCCCCEEE
ILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESLINAYDNTIFYNDYLLDKIIS
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCEEEEHHHHHHHHHH
MLENAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHSII
HHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHE
QTQTPINQNVIFHSVLGVFLDFKNPSVVYRPSLDLLKHKKE
ECCCCCCCHHHHHHHHHHHEECCCCCEEECCCHHHHHCCCC
>Mature Secondary Structure 
ASLFHLRFLKPLSCLQAGLLYSLIFGVLYHFPLFVYVYKESNQVSFIAMMVVVLFCVNG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHCH
ALFLALGLISASLMRWSAIVFSLLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFIFIASALANTKNWLWFDKHA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHH
KFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GYQKPTTPRLSKRLADNELTLFNATSCATYTTASLECILDSSFKNNAYENLPTYLTKAGI
ECCCCCCHHHHHHHCCCCEEEEECCCCCEEECCEEEEEECCCCCCCCHHHHHHHHHHCCC
KVFWYSANDGEKNVKVTSYLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLL
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHCCCCCEEE
ILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESLINAYDNTIFYNDYLLDKIIS
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCEEEEHHHHHHHHHH
MLENAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHSII
HHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHE
QTQTPINQNVIFHSVLGVFLDFKNPSVVYRPSLDLLKHKKE
ECCCCCCCHHHHHHHHHHHEECCCCCEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]