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The map label for this gene is pnp

Identifier: 15618907

GI number: 15618907

Start: 1150763

End: 1152847

Strand: Reverse

Name: pnp

Synonym: CPn0999

Alternate gene names: 15618907

Gene position: 1152847-1150763 (Counterclockwise)

Preceding gene: 15618908

Following gene: 15618904

Centisome position: 93.71

GC content: 40.48

Gene sequence:

>2085_bases
ATGAATTTTCAAACTATTTCTATTAATCTGACCGAAGGTAAAATATTGGTGTTTGAAACAGGGAAAATTGCTCGTCAAGC
TAACGGAGCTGTTCTTGTCCGTTCAGGGGAAACCTGTGTTTTTGCAAGTGCATGCGCTGTAGATCTTGATGACAAAGTAG
ACTTTCTTCCTTTACGAGTGGACTACCAAGAAAAATTCTCTTCCACAGGGAAAACTCTTGGTGGATTTATAAAAAGAGAA
GGACGCCCCTCTGAAAAAGAAATTTTAGTTTCTCGATTGATTGATCGGTCTCTACGTCCTTCCTTCCCCTACCGACTCAT
GCAAGATGTGCAAGTTTTATCTTATGTATGGTCGTATGACGGTCAAGTTTTACCTGATCCTCTTGCTATCTGCGCAGCAT
CTGCTGCTTTAGCAATTTCAGATATTCCACAGAGCAACATTGTTGCTGGAGTACGGATTGGATGTATTGATAACCAATGG
GTCATCAATCCTACAAAAACTGAGTTAGCATCCTCTACTTTAGATCTTGTATTAGCTGGAACAGAAAACGCTATTCTAAT
GATAGAAGGTCATTGCGATTTCTTTACCGAAGAGCAAGTACTTGATGCCATAGAATTTGGTCACAAGCATATTGTTACCA
TCTGTAAAAGATTACAGTTGTGGCAAGAAGAAGTTGGAAAATCTAAAAATCTAAGTGCTGTATATCCTCTTCCTGCAGAA
GTTTTAACAGCTGTGAAAGAGTGTGCGCAAGACAAGTTTACTGAACTCTTTAATATTAAAGATAAAAAAGTACATGCAGC
TACTGCGCATGAGATTGAAGAAAATATTTTAGAAAAATTACAGCGCGAAGATGATGATCTTTTCTCTTCTTTTAACATCA
AAGCGGCATGTAAAACTTTAAAATCTGACACGATGCGTGCTCTCATAAGAGATAGAGAGATCCGTGCAGATGGACGTTCT
TTAACTACAGTCCGTCCTATTACTATTGAAACTTCTTACCTTCCTCGTACTCACGGAAGCTGCCTCTTCACTCGTGGAGA
GACTCAGACACTTGCGGTATGTACTTTAGGCAGTGAGGCAATGGCTCAACGCTATGAGGATTTGAATGGTGAAGGACTTT
CAAAATTTTACTTACAATACTTCTTCCCTCCCTTCTCTGTTGGAGAGGTAGGAAGAATCGGCTCTCCAGGAAGGAGAGAA
ATTGGCCATGGCAAATTGGCAGAGAAGGCTTTAAGTCATGCTCTTCCTGATAGCGCTACATTTCCCTATACCATCCGTAT
AGAATCTAATATTACAGAATCTAATGGTTCTTCATCCATGGCTTCTGTATGTGGCGGTTGCCTCGCTCTTATGGACGCTG
GCGTCCCTATTTCTTCTCCTATTGCAGGAATTGCTATGGGATTGATCCTTGATGATCAAGGAGCGATTATCCTTTCTGAT
ATTTCCGGTCTTGAAGATCACCTTGGAGACATGGACTTTAAAATAGCAGGGAGTGGTAAAGGAATTACAGCCTTCCAAAT
GGATATCAAAGTTGAAGGAATCACTCCAGCTATCATGAAGAAAGCTCTTTCCCAAGCGAAGCAAGGGTGTAATGATATTT
TAAATATTATGAACGAAGCCCTTTCAGCTCCCAAAGCAGACCTATCTCAATACGCGCCTCGTATTGAAACAATGCAAATT
AAACCAACAAAAATTGCTTCAGTAATTGGGCCGGGAGGCAAGCAAATCCGTCAGATCATTGAAGAGACTGGCGTCCAAAT
TGATGTTAACGACTTAGGAGTTGTTAGCATCTCTGCGTCCTCAGCATCAGCTATCAATAAAGCTAAAGAAATCATTGAAG
GTTTAGTTGGAGAAGTTGAAGTTGGAAAAACCTACAGAGGTCGCGTAACTTCCGTAGTTGCGTTTGGAGCCTTTGTTGAA
GTTCTCCCCGGAAAAGAAGGACTCTGCCACATCTCTGAGTGTTCTAGACAGCGAATAGAAAATATAAGTGATGTTGTTAA
GGAAGGCGATATCATTGATGTAAAACTCTTAAGCATCAATGAAAAAGGTCAACTTAAGTTGAGCCATAAAGCAACTCTAG
AATAA

Upstream 100 bases:

>100_bases
AATTCTACAGATACCGAAAGGTACAAAAATTTAATTACAAGATTGAATCTACGTAAGTAGTTTTTTCGTTCCCTTTACAA
ATATCTAACAGGAGACCTTC

Downstream 100 bases:

>100_bases
TCTTTCACATACATTCTATTCTATACGCTTAACATGAAAAGCTAGTCTTCTTAGAAAGACTAGCTTTTTTTATGATTACA
TCTAACTCTAATCTGATTCA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 694; Mature: 694

Protein sequence:

>694_residues
MNFQTISINLTEGKILVFETGKIARQANGAVLVRSGETCVFASACAVDLDDKVDFLPLRVDYQEKFSSTGKTLGGFIKRE
GRPSEKEILVSRLIDRSLRPSFPYRLMQDVQVLSYVWSYDGQVLPDPLAICAASAALAISDIPQSNIVAGVRIGCIDNQW
VINPTKTELASSTLDLVLAGTENAILMIEGHCDFFTEEQVLDAIEFGHKHIVTICKRLQLWQEEVGKSKNLSAVYPLPAE
VLTAVKECAQDKFTELFNIKDKKVHAATAHEIEENILEKLQREDDDLFSSFNIKAACKTLKSDTMRALIRDREIRADGRS
LTTVRPITIETSYLPRTHGSCLFTRGETQTLAVCTLGSEAMAQRYEDLNGEGLSKFYLQYFFPPFSVGEVGRIGSPGRRE
IGHGKLAEKALSHALPDSATFPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPISSPIAGIAMGLILDDQGAIILSD
ISGLEDHLGDMDFKIAGSGKGITAFQMDIKVEGITPAIMKKALSQAKQGCNDILNIMNEALSAPKADLSQYAPRIETMQI
KPTKIASVIGPGGKQIRQIIEETGVQIDVNDLGVVSISASSASAINKAKEIIEGLVGEVEVGKTYRGRVTSVVAFGAFVE
VLPGKEGLCHISECSRQRIENISDVVKEGDIIDVKLLSINEKGQLKLSHKATLE

Sequences:

>Translated_694_residues
MNFQTISINLTEGKILVFETGKIARQANGAVLVRSGETCVFASACAVDLDDKVDFLPLRVDYQEKFSSTGKTLGGFIKRE
GRPSEKEILVSRLIDRSLRPSFPYRLMQDVQVLSYVWSYDGQVLPDPLAICAASAALAISDIPQSNIVAGVRIGCIDNQW
VINPTKTELASSTLDLVLAGTENAILMIEGHCDFFTEEQVLDAIEFGHKHIVTICKRLQLWQEEVGKSKNLSAVYPLPAE
VLTAVKECAQDKFTELFNIKDKKVHAATAHEIEENILEKLQREDDDLFSSFNIKAACKTLKSDTMRALIRDREIRADGRS
LTTVRPITIETSYLPRTHGSCLFTRGETQTLAVCTLGSEAMAQRYEDLNGEGLSKFYLQYFFPPFSVGEVGRIGSPGRRE
IGHGKLAEKALSHALPDSATFPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPISSPIAGIAMGLILDDQGAIILSD
ISGLEDHLGDMDFKIAGSGKGITAFQMDIKVEGITPAIMKKALSQAKQGCNDILNIMNEALSAPKADLSQYAPRIETMQI
KPTKIASVIGPGGKQIRQIIEETGVQIDVNDLGVVSISASSASAINKAKEIIEGLVGEVEVGKTYRGRVTSVVAFGAFVE
VLPGKEGLCHISECSRQRIENISDVVKEGDIIDVKLLSINEKGQLKLSHKATLE
>Mature_694_residues
MNFQTISINLTEGKILVFETGKIARQANGAVLVRSGETCVFASACAVDLDDKVDFLPLRVDYQEKFSSTGKTLGGFIKRE
GRPSEKEILVSRLIDRSLRPSFPYRLMQDVQVLSYVWSYDGQVLPDPLAICAASAALAISDIPQSNIVAGVRIGCIDNQW
VINPTKTELASSTLDLVLAGTENAILMIEGHCDFFTEEQVLDAIEFGHKHIVTICKRLQLWQEEVGKSKNLSAVYPLPAE
VLTAVKECAQDKFTELFNIKDKKVHAATAHEIEENILEKLQREDDDLFSSFNIKAACKTLKSDTMRALIRDREIRADGRS
LTTVRPITIETSYLPRTHGSCLFTRGETQTLAVCTLGSEAMAQRYEDLNGEGLSKFYLQYFFPPFSVGEVGRIGSPGRRE
IGHGKLAEKALSHALPDSATFPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPISSPIAGIAMGLILDDQGAIILSD
ISGLEDHLGDMDFKIAGSGKGITAFQMDIKVEGITPAIMKKALSQAKQGCNDILNIMNEALSAPKADLSQYAPRIETMQI
KPTKIASVIGPGGKQIRQIIEETGVQIDVNDLGVVSISASSASAINKAKEIIEGLVGEVEVGKTYRGRVTSVVAFGAFVE
VLPGKEGLCHISECSRQRIENISDVVKEGDIIDVKLLSINEKGQLKLSHKATLE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=715, Percent_Identity=37.4825174825175, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=680, Percent_Identity=45.1470588235294, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI157672248, Length=193, Percent_Identity=26.4248704663212, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=731, Percent_Identity=30.6429548563612, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI281362905, Length=712, Percent_Identity=36.938202247191, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=712, Percent_Identity=36.938202247191, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=712, Percent_Identity=36.938202247191, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=648, Percent_Identity=37.5, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHLPN (Q9Z6R0)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D86615
- PIR:   G72009
- RefSeq:   NP_225193.1
- RefSeq:   NP_301054.1
- RefSeq:   NP_445393.1
- RefSeq:   NP_877309.1
- HSSP:   P05055
- ProteinModelPortal:   Q9Z6R0
- PHCI-2DPAGE:   Q9Z6R0
- GeneID:   1467716
- GeneID:   894682
- GeneID:   919768
- GeneID:   963295
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0855
- KEGG:   cpn:CPn0999
- KEGG:   cpt:CpB1037
- TIGR:   CP_0855
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   CPNE115711:CP_0855-MONOMER
- BioCyc:   CPNE115713:CPN0999-MONOMER
- BioCyc:   CPNE138677:CPJ0999-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75348; Mature: 75348

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQTISINLTEGKILVFETGKIARQANGAVLVRSGETCVFASACAVDLDDKVDFLPLRV
CCEEEEEEEEECCEEEEEECCCHHHCCCCEEEEECCCEEEEEEEEEECCCCCCCEEEEEE
DYQEKFSSTGKTLGGFIKREGRPSEKEILVSRLIDRSLRPSFPYRLMQDVQVLSYVWSYD
CHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
GQVLPDPLAICAASAALAISDIPQSNIVAGVRIGCIDNQWVINPTKTELASSTLDLVLAG
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEECCCHHHHHHCCEEEEEEC
TENAILMIEGHCDFFTEEQVLDAIEFGHKHIVTICKRLQLWQEEVGKSKNLSAVYPLPAE
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHH
VLTAVKECAQDKFTELFNIKDKKVHAATAHEIEENILEKLQREDDDLFSSFNIKAACKTL
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHH
KSDTMRALIRDREIRADGRSLTTVRPITIETSYLPRTHGSCLFTRGETQTLAVCTLGSEA
HHHHHHHHHHCCHHCCCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEECCHHH
MAQRYEDLNGEGLSKFYLQYFFPPFSVGEVGRIGSPGRREIGHGKLAEKALSHALPDSAT
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
FPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPISSPIAGIAMGLILDDQGAIILSD
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEECCCCCEEEEC
ISGLEDHLGDMDFKIAGSGKGITAFQMDIKVEGITPAIMKKALSQAKQGCNDILNIMNEA
CCCHHHHHCCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSAPKADLSQYAPRIETMQIKPTKIASVIGPGGKQIRQIIEETGVQIDVNDLGVVSISAS
HCCCHHHHHHHCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCEEEEEECC
SASAINKAKEIIEGLVGEVEVGKTYRGRVTSVVAFGAFVEVLPGKEGLCHISECSRQRIE
CHHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
NISDVVKEGDIIDVKLLSINEKGQLKLSHKATLE
HHHHHHHCCCEEEEEEEEECCCCCEEECCCCCCC
>Mature Secondary Structure
MNFQTISINLTEGKILVFETGKIARQANGAVLVRSGETCVFASACAVDLDDKVDFLPLRV
CCEEEEEEEEECCEEEEEECCCHHHCCCCEEEEECCCEEEEEEEEEECCCCCCCEEEEEE
DYQEKFSSTGKTLGGFIKREGRPSEKEILVSRLIDRSLRPSFPYRLMQDVQVLSYVWSYD
CHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
GQVLPDPLAICAASAALAISDIPQSNIVAGVRIGCIDNQWVINPTKTELASSTLDLVLAG
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEECCCHHHHHHCCEEEEEEC
TENAILMIEGHCDFFTEEQVLDAIEFGHKHIVTICKRLQLWQEEVGKSKNLSAVYPLPAE
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHH
VLTAVKECAQDKFTELFNIKDKKVHAATAHEIEENILEKLQREDDDLFSSFNIKAACKTL
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHH
KSDTMRALIRDREIRADGRSLTTVRPITIETSYLPRTHGSCLFTRGETQTLAVCTLGSEA
HHHHHHHHHHCCHHCCCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEECCHHH
MAQRYEDLNGEGLSKFYLQYFFPPFSVGEVGRIGSPGRREIGHGKLAEKALSHALPDSAT
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
FPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPISSPIAGIAMGLILDDQGAIILSD
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEECCCCCEEEEC
ISGLEDHLGDMDFKIAGSGKGITAFQMDIKVEGITPAIMKKALSQAKQGCNDILNIMNEA
CCCHHHHHCCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSAPKADLSQYAPRIETMQIKPTKIASVIGPGGKQIRQIIEETGVQIDVNDLGVVSISAS
HCCCHHHHHHHCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCEEEEEECC
SASAINKAKEIIEGLVGEVEVGKTYRGRVTSVVAFGAFVEVLPGKEGLCHISECSRQRIE
CHHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
NISDVVKEGDIIDVKLLSINEKGQLKLSHKATLE
HHHHHHHCCCEEEEEEEEECCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362