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The map label for this gene is pcnB_2

Identifier: 15618873

GI number: 15618873

Start: 1106745

End: 1108037

Strand: Reverse

Name: pcnB_2

Synonym: CPn0966

Alternate gene names: 15618873

Gene position: 1108037-1106745 (Counterclockwise)

Preceding gene: 15618880

Following gene: 15618872

Centisome position: 90.07

GC content: 37.59

Gene sequence:

>1293_bases
ATGGTCTGCGAAAACAATATTCTCTCTGGTAGAGGCCTAGAATTGTTAAAAAAAAAATCTAATATCACTTTAACTCCTAC
GATCTATTCTGTTTCTAACCATAACATCAAACTCAAAGATTTCTCTCCTCACGCCCTGTCAGTGATCAAAACCCTCCGTA
AAGCAGGGTATATCGCTTATATTGTTGGGGGTTGCATTAGGGATTTGTTATTAAATACCACGCCGAAGGATTTTGATATC
TCCACTTCAGCGAAACCTGAAGAAATTAAAGCTATCTTTAAAAATTGTATTTTGGTCGGTAAGCGTTTCCGTCTTGCCCA
TATTAGATTCTCTAAGCAGATTATTGAAGTTTCAACATTTCGTTCGGGAAGCACCGATGAAGATGTCTTGATTACTAAAG
ATAATCTATGGGGGACTCCTGAAGAAGATGTCTTGCGAAGAGATTTTACAATCAATGGATTGTTTTACGATCCCGAGCAT
GAAGAGATTATAGACTATACTGGTGGCGTTAATGATTTAAGGAACCGTTATTTGCGCACTATCGGCGATCCCTTTACAAG
ATTCAAGCAAGATCCAGTACGCATGCTGAGACTGTTAAAGATTTTATCACGGTCGCCGTTTACTGTAGAAACGCAAACTC
AAGAAGCTTTAATTGCTTGTCGCCAAGAGCTAATTAAAAGTTCTCAGGCACGGGTCTTCGAAGAACTTATCAAAATGTTA
AATTCCGGAGCCGCAAAGAACTTTTTCCAACTGCTAATAGAGAACCATCTTCTTGAGATCCTTTTTCCCTATATGGATAA
GGCATTCCGCTTAAATCGCGCACTAGAAGAGCAAACAGCCACCTACCTAAAAGCTCTAGATGACAAAATACTAAAAAAAG
AGGCAGAGTATGATCGTCATCAGCTGATGGCTATATTCCTTTTTCCTTTAGTGAACTTTAATGTCCGTTATAAGCATCAG
AAGCACCCGTATTTGTCTTTGACATCCGTTTTTGATTATATCAAAAATTTCCTAGAACAATTCTTTGCTGACTCGTTCAC
AAGTTGCTCAAAAAAGAATTTTATCCTGACAGCATTAATTTTACAAATGCAGTACAGACTCACTCCCCTGATCCCTACAA
AAAAAGCGCTCTTCTTTAATAAGAAACTTTTACATCACACAAGGTTCTTAGAAGCATTATCTTTATTGGAAATTCGTAGT
ATAGTGTATCCTAAGCTCGATAAAGTGTATGTTGCTTGGATACGTCATCATCAAACACTTAAGTGTAAAAAGGATTCTCA
TTCTCAAAAGTAG

Upstream 100 bases:

>100_bases
AACTTCCCTAGTTTTGCTATGTTGGGATTTTAAAATGGAAGTAGACGCAAACTACACACTCTTTTTCTTCCAAGACTTTT
AACTCTTAATAACTATAATA

Downstream 100 bases:

>100_bases
GTCTAAACATGATCCCTTCTGGCCTAGTTTATCTGCTTTATCCCCTAGGATTTCTTGCTAGTCTTTTTTTTGGGAGTGCC
TTCTCAATACAGTGGTGGCT

Product: Poly A polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MVCENNILSGRGLELLKKKSNITLTPTIYSVSNHNIKLKDFSPHALSVIKTLRKAGYIAYIVGGCIRDLLLNTTPKDFDI
STSAKPEEIKAIFKNCILVGKRFRLAHIRFSKQIIEVSTFRSGSTDEDVLITKDNLWGTPEEDVLRRDFTINGLFYDPEH
EEIIDYTGGVNDLRNRYLRTIGDPFTRFKQDPVRMLRLLKILSRSPFTVETQTQEALIACRQELIKSSQARVFEELIKML
NSGAAKNFFQLLIENHLLEILFPYMDKAFRLNRALEEQTATYLKALDDKILKKEAEYDRHQLMAIFLFPLVNFNVRYKHQ
KHPYLSLTSVFDYIKNFLEQFFADSFTSCSKKNFILTALILQMQYRLTPLIPTKKALFFNKKLLHHTRFLEALSLLEIRS
IVYPKLDKVYVAWIRHHQTLKCKKDSHSQK

Sequences:

>Translated_430_residues
MVCENNILSGRGLELLKKKSNITLTPTIYSVSNHNIKLKDFSPHALSVIKTLRKAGYIAYIVGGCIRDLLLNTTPKDFDI
STSAKPEEIKAIFKNCILVGKRFRLAHIRFSKQIIEVSTFRSGSTDEDVLITKDNLWGTPEEDVLRRDFTINGLFYDPEH
EEIIDYTGGVNDLRNRYLRTIGDPFTRFKQDPVRMLRLLKILSRSPFTVETQTQEALIACRQELIKSSQARVFEELIKML
NSGAAKNFFQLLIENHLLEILFPYMDKAFRLNRALEEQTATYLKALDDKILKKEAEYDRHQLMAIFLFPLVNFNVRYKHQ
KHPYLSLTSVFDYIKNFLEQFFADSFTSCSKKNFILTALILQMQYRLTPLIPTKKALFFNKKLLHHTRFLEALSLLEIRS
IVYPKLDKVYVAWIRHHQTLKCKKDSHSQK
>Mature_430_residues
MVCENNILSGRGLELLKKKSNITLTPTIYSVSNHNIKLKDFSPHALSVIKTLRKAGYIAYIVGGCIRDLLLNTTPKDFDI
STSAKPEEIKAIFKNCILVGKRFRLAHIRFSKQIIEVSTFRSGSTDEDVLITKDNLWGTPEEDVLRRDFTINGLFYDPEH
EEIIDYTGGVNDLRNRYLRTIGDPFTRFKQDPVRMLRLLKILSRSPFTVETQTQEALIACRQELIKSSQARVFEELIKML
NSGAAKNFFQLLIENHLLEILFPYMDKAFRLNRALEEQTATYLKALDDKILKKEAEYDRHQLMAIFLFPLVNFNVRYKHQ
KHPYLSLTSVFDYIKNFLEQFFADSFTSCSKKNFILTALILQMQYRLTPLIPTKKALFFNKKLLHHTRFLEALSLLEIRS
IVYPKLDKVYVAWIRHHQTLKCKKDSHSQK

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=234, Percent_Identity=28.6324786324786, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081691, Length=391, Percent_Identity=35.8056265984655, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=32.579185520362, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=253, Percent_Identity=27.6679841897233, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321016, Length=229, Percent_Identity=31.0043668122271, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281360112, Length=299, Percent_Identity=28.4280936454849, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357337, Length=299, Percent_Identity=28.4280936454849, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50123; Mature: 50123

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCENNILSGRGLELLKKKSNITLTPTIYSVSNHNIKLKDFSPHALSVIKTLRKAGYIAY
CCCCCCCCCCCCHHHHHHCCCEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCHHHH
IVGGCIRDLLLNTTPKDFDISTSAKPEEIKAIFKNCILVGKRFRLAHIRFSKQIIEVSTF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
RSGSTDEDVLITKDNLWGTPEEDVLRRDFTINGLFYDPEHEEIIDYTGGVNDLRNRYLRT
CCCCCCCCEEEEECCCCCCCHHHHHHHCEEEECEEECCCHHHHHHHCCCHHHHHHHHHHH
IGDPFTRFKQDPVRMLRLLKILSRSPFTVETQTQEALIACRQELIKSSQARVFEELIKML
HCCHHHHHCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSGAAKNFFQLLIENHLLEILFPYMDKAFRLNRALEEQTATYLKALDDKILKKEAEYDRH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMAIFLFPLVNFNVRYKHQKHPYLSLTSVFDYIKNFLEQFFADSFTSCSKKNFILTALI
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LQMQYRLTPLIPTKKALFFNKKLLHHTRFLEALSLLEIRSIVYPKLDKVYVAWIRHHQTL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCE
KCKKDSHSQK
EECCCCCCCC
>Mature Secondary Structure
MVCENNILSGRGLELLKKKSNITLTPTIYSVSNHNIKLKDFSPHALSVIKTLRKAGYIAY
CCCCCCCCCCCCHHHHHHCCCEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCHHHH
IVGGCIRDLLLNTTPKDFDISTSAKPEEIKAIFKNCILVGKRFRLAHIRFSKQIIEVSTF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
RSGSTDEDVLITKDNLWGTPEEDVLRRDFTINGLFYDPEHEEIIDYTGGVNDLRNRYLRT
CCCCCCCCEEEEECCCCCCCHHHHHHHCEEEECEEECCCHHHHHHHCCCHHHHHHHHHHH
IGDPFTRFKQDPVRMLRLLKILSRSPFTVETQTQEALIACRQELIKSSQARVFEELIKML
HCCHHHHHCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSGAAKNFFQLLIENHLLEILFPYMDKAFRLNRALEEQTATYLKALDDKILKKEAEYDRH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMAIFLFPLVNFNVRYKHQKHPYLSLTSVFDYIKNFLEQFFADSFTSCSKKNFILTALI
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LQMQYRLTPLIPTKKALFFNKKLLHHTRFLEALSLLEIRSIVYPKLDKVYVAWIRHHQTL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCE
KCKKDSHSQK
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]