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The map label for this gene is fmt

Identifier: 15618559

GI number: 15618559

Start: 731707

End: 732672

Strand: Reverse

Name: fmt

Synonym: CPn0649

Alternate gene names: 15618559

Gene position: 732672-731707 (Counterclockwise)

Preceding gene: 15618560

Following gene: 15618558

Centisome position: 59.56

GC content: 44.72

Gene sequence:

>966_bases
TTGAATCTTAAGGTTGTCTATTTCGGCACACCCACATTTGCAGCCACCGTTTTGCAAGATTTGTTGCATCACAAAATCCA
AATTACAGCTGTTGTTACCCGAGTTGATAAGCCCCAAAAAAGATCGGCACAACTCATTCCTTCTCCAGTAAAAACTATAG
CTCTAACTCACGGTCTACCTTTACTCCAACCTAGTAAAGCGTCAGACCCCCAATTTATTGAAGAACTTCGGGCTTTTAAT
GCTGATGTTTTTATTGTGGTTGCCTATGGAGCGATTCTTCGTCAGATAGTCCTCGATATTCCTCGTTATGGTTGTTACAA
TCTGCATGCAGGGCTCTTGCCAGCCTACCGAGGCGCAGCTCCCATACAACGCTGTATTATGGAAGGAGCTACAGAATCTG
GAAATACTGTCATCCGTATGGATGCAGGTATGGATACAGGCGACATGGCAAATATTACGCGTGTCCCCATAGGTCCTGAT
ATGACTTCAGGAGAATTGGCGGATGCTCTTGCATCGCAGGGCGCAGAAGTTCTAATAAAAACTTTACAACAAATTGAGTC
AGGTCAACTGCAACTCGTCAGTCAAGACGCTGCTTTGGCGACAATAGCGCCAAAATTGTCTAAGGAAGAAGGACAGGTTC
CTTGGGATAAACCTGCTAAAGAGGCTTATGCTCATATACGAGGAGTCACGCCAGCTCCAGGAGCGTGGACCCTATTCTCT
TTTTCTGAAAAAGCGCCAAAACGCTTGATGATCCGTAAGGCCTCTCTTTTAGCAGAGGCGGGAAGATATGGAGCCCCCGG
GACTGTTGTTGTAACAGATAGGCAAGAGCTTGCTATTGCCTGTTCTGAGGGAGCCATTTGCTTGCACGAAGTGCAGGTAG
AAGGCAAGGGTTCTACGAACTCAAAATCCTTTTTAAACGGCTATCCAGCAAAGAAATTAAAAATAGTTTTTACTTTAAAT
AATTAA

Upstream 100 bases:

>100_bases
TTCATTGAGTTTTGTCAAAGCCCAAGTAAGCGCGGCATCGAACGAAGCATAGATAAGCAAGCCCTTGAAGAAGAAAGCGC
AGACAAAGAAGGAGTTTTGA

Downstream 100 bases:

>100_bases
CTTTACAACGTGTTTTTTATTTAGGGCATTTAAACTAAATTAAGTCGTTTTATGTTATTATAGGCTCTAATTTTTTTTTA
AAAAGCCTTGTAAGGAGGTA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLPLLQPSKASDPQFIEELRAFN
ADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAAPIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPD
MTSGELADALASQGAEVLIKTLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAIACSEGAICLHEVQVEGKGSTNSKSFLNGYPAKKLKIVFTLN
N

Sequences:

>Translated_321_residues
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLPLLQPSKASDPQFIEELRAFN
ADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAAPIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPD
MTSGELADALASQGAEVLIKTLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAIACSEGAICLHEVQVEGKGSTNSKSFLNGYPAKKLKIVFTLN
N
>Mature_321_residues
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLPLLQPSKASDPQFIEELRAFN
ADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAAPIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPD
MTSGELADALASQGAEVLIKTLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAIACSEGAICLHEVQVEGKGSTNSKSFLNGYPAKKLKIVFTLN
N

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=321, Percent_Identity=27.1028037383178, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI21614513, Length=241, Percent_Identity=25.7261410788382, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI238814322, Length=241, Percent_Identity=25.3112033195021, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=40.5228758169935, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=27.3684210526316, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI133930964, Length=252, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550868, Length=325, Percent_Identity=27.3846153846154, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28571984, Length=279, Percent_Identity=27.5985663082437, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585660, Length=250, Percent_Identity=24.8, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_CHLPN (Q9Z7Q5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72051
- PIR:   F81615
- PIR:   F86571
- RefSeq:   NP_224845.1
- RefSeq:   NP_300705.1
- RefSeq:   NP_444650.1
- RefSeq:   NP_876947.1
- ProteinModelPortal:   Q9Z7Q5
- SMR:   Q9Z7Q5
- GeneID:   1467354
- GeneID:   895283
- GeneID:   919399
- GeneID:   962917
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0098
- KEGG:   cpn:CPn0649
- KEGG:   cpt:CpB0675
- TIGR:   CP_0098
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- PhylomeDB:   Q9Z7Q5
- ProtClustDB:   PRK00005
- BioCyc:   CPNE115711:CP_0098-MONOMER
- BioCyc:   CPNE115713:CPN0649-MONOMER
- BioCyc:   CPNE138677:CPJ0649-MONOMER
- BioCyc:   CPNE182082:CPB0675-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34540; Mature: 34540

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLP
CCEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
LLQPSKASDPQFIEELRAFNADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAA
CCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHH
PIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPDMTSGELADALASQGAEVLIK
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHH
TLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
HHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEE
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAIACSEGAICLHEVQVEGKGSTN
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEEECCCCCC
SKSFLNGYPAKKLKIVFTLNN
CCHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLP
CCEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
LLQPSKASDPQFIEELRAFNADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAA
CCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHH
PIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPDMTSGELADALASQGAEVLIK
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHH
TLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
HHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEE
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAIACSEGAICLHEVQVEGKGSTN
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEEECCCCCC
SKSFLNGYPAKKLKIVFTLNN
CCHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362