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The map label for this gene is rpoA

Identifier: 15618536

GI number: 15618536

Start: 718492

End: 719616

Strand: Reverse

Name: rpoA

Synonym: CPn0626

Alternate gene names: 15618536

Gene position: 719616-718492 (Counterclockwise)

Preceding gene: 15618537

Following gene: 15618535

Centisome position: 58.49

GC content: 36.62

Gene sequence:

>1125_bases
ATGTCAGATAACGCACACAATTTACTTTATGATAAGTTTGAACTGCCTGAAGCAGTTAAAATGTTGCCTGTTGAAGGGCT
TCCAATAGACAAACATGCTCGCTTCATTGCTGAGCCTCTAGAAAGAGGAATGGGCCACACTTTAGGAAATGCTCTAAGAC
GTGCTTTGCTTATTGGTTTAGAAGCTCCAGCTATTATCTCGTTTGCTATGACAGGCGTACTTCATGAATATATGGCAATC
GAAGGGGTTATTGAGGATGTAACTAACATCATTCTGAATTTAAAAGGTGCCCTATTAAAAAAGTACCCCATGCAGGATAG
TTCTTTAGGAAGAACCACTCAGGTCTTAAAAGCTTCAATTTCTATAGATGCTTCCGATTTAGCCGCAGCTAATGGACAAA
AAGAAGTGACTCTACAAGATCTATTGCAAGAAGGGGACTTTGAAGCCGTTAACCCAGATCAAGTCATTTTTACTGTTACT
CAACCCATACAGTTGGAAGTCGTTCTACGAATTGCTTTTGGTAGGGGATATACACCTTCTGAAAGGATTGTTTTGGAAGA
TAAGGGTGTTTATGAAATCGTTTTGGATGCAGCCTTTTCTCCAGTCACTTTAGTCAACTACTTTGTAGAAGATACACGGG
TGGGTCAGGATACAGATTTTGACCGTTTAGTTTTAATAGTTGAAACAGATGGAAGAGTGACTCCGAAAGAAGCTCTGGCT
TTTTCAACTCAAATTTTGACTAAACATTTTTCCATTTTTGAAAATATGGATGAGAAGAAAATCGTATTTGAAGAAGCTAT
TTCTATTGAGAAAGAAAACAAAGATGATATTCTTCATAAGTTAATTTTAGGAATTAATGAAATAGAACTCTCAGTCAGAT
CAACAAATTGTTTGTCTAATGCAAATATTGAGACTATTGGCGAACTTGTTATTATGCCTGAACCTCGATTGCTACAATTC
AGAAATTTTGGAAAGAAATCACTGTGTGAGATCAAGAATAAATTGAAAGAAATGAAGCTTGAATTAGGAATGGACCTAAC
GCAATTTGGCGTAGGTTTAGATAACGTAAAAGAAAAAATGAAGTGGTATGCCGAAAAGATTCGGGCTAAAAATACAAAGG
GATAG

Upstream 100 bases:

>100_bases
GTTTGGTAGTTTCTGTAATCCGTGATGAAACTCCTGTTCCTCATAATGGTTGCCGGCCAAGAAAAAGGCGCAGAGTGTAG
TTTTAGGGAAGGAGAAAGGG

Downstream 100 bases:

>100_bases
TAAGTAATGCAACACGCTAGAAAAAAATTTAGAGTTGGTCGTACTTCCTCGCATAATCGCTGTATGTTAGCTAACATGTT
AAAGTCTCTAATTCATTATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAI
EGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVT
QPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQF
RNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNTKG

Sequences:

>Translated_374_residues
MSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAI
EGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVT
QPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQF
RNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNTKG
>Mature_373_residues
SDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAIE
GVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQ
PIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALAF
STQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQFR
NFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNTKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=38.5093167701863, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 41863; Mature: 41732

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGL
CCCCCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
EAPAIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
SIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPS
CCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCC
ERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
CEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHH
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
ANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKM
CCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
KWYAEKIRAKNTKG
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGL
CCCCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
EAPAIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
SIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPS
CCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCC
ERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
CEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHH
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
ANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKM
CCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
KWYAEKIRAKNTKG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]